################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 1193 AAATTC [GA]^AATT[CT] AflIII 1283 ACATGT A^C[GA][CT]GT AluI 429 AGCT AG^CT AlwNI 389 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1193 AAATTC [GA]^AATT[CT] AseI 736 ATTAAT AT^TAAT AsnI 736 ATTAAT AT^TAAT Asp700I 1189 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 549 GGATCC G^GATCC BanI 355 GGTGCC G^G[CT][GA]CC BanII 22 GGGCTC G[GA]GC[CT]^C BbsI 105 GAAGAC GAAGAC(2/6) BbvCI 319 GCTGAGG CCTCAGC(-5/-2) BcgI 1393 GCATGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 22 GGGCTC G[AGT]GC[ACT]^C BpmI 905 CTCCAG CTGGAG(16/14) BpuAI 105 GAAGAC GAAGAC(2/6) BsaAI 273 TACGTA [CT]AC^GT[GA] BsaWI 11 ACCGGA [AT]^CCGG[AT] BsgI 936 CTGCAC GTGCAG(16/14) BsmFI 1258 GGGAC GGGAC(10/14) Bsp1286I 22 GGGCTC G[AGT]GC[ACT]^C BspLU11I 1283 ACATGT A^CATGT BstBI 364 TTCGAA TT^CGAA Cac8I 1393 GCATGC GC[ACGT]^[ACGT]GC DraI 151 TTTAAA TTT^AAA DraII 264 AGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 325 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1215 GAAGAG CTCTTC(1/4) EcoO109I 264 AGGTCCT [GA]G^G[ACGT]CC[CT] HapII 12 CCGG C^CGG HgaI 316 GACGC GACGC(5/10) HincII 1165 GTCAAC GT[CT]^[GA]AC HindII 1165 GTCAAC GT[CT]^[GA]AC HpaII 12 CCGG C^CGG Ksp632I 1215 GAAGAG CTCTTC(1/4) MfeI 143 CAATTG C^AATTG MspA1I 428 CAGCTG C[AC]G^C[GT]G MspI 12 CCGG C^CGG MunI 143 CAATTG C^AATTG NdeI 971 CATATG CA^TATG NspV 364 TTCGAA TT^CGAA PpuMI 264 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 736 ATTAAT AT^TAAT PvuII 428 CAGCTG CAG^CTG SfuI 364 TTCGAA TT^CGAA SnaBI 273 TACGTA TAC^GTA SphI 1393 GCATGC GCATG^C StuI 893 AGGCCT AGG^CCT XmnI 1189 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaII 265 GGTCC G^G[AT]CC 1259 GGACC BfaI 292 CTAG C^TAG 982 CTAG BsaI 89 GAGACC GGTCTC(1/5) 1008 GAGACC BsiYI 1066 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1123 CCTATAACTGG BslI 1066 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1123 CCTATAACTGG Bsp1407I 964 TGTACA T^GTACA 1016 TGTACA BsrGI 964 TGTACA T^GTACA 1016 TGTACA BstF5I 434 CATCC GGATG(2/0) 887 CATCC BstYI 197 GGATCT [GA]^GATC[CT] 549 GGATCC Cfr13I 265 GGTCC G^G[ACGT]CC 1259 GGACC DdeI 320 CTGAG C^T[ACGT]AG 1345 CTCAG FokI 434 CATCC GGATG(9/13) 887 CATCC HaeIII 894 GGCC GG^CC 1324 GGCC HphI 1267 GGTGA GGTGA(8/7) 1359 GGTGA MaeI 292 CTAG C^TAG 982 CTAG MaeII 274 ACGT A^CGT 845 ACGT MflI 197 GGATCT [GA]^GATC[CT] 549 GGATCC MwoI 49 GCTGTCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 421 GCTGTGGCAGC NspI 1283 ACATGT [GA]CATG^[CT] 1393 GCATGC Sau96I 265 GGTCC G^G[ACGT]CC 1259 GGACC SfaNI 748 GATGC GCATC(5/9) 433 GCATC SspBI 964 TGTACA T^GTACA 1016 TGTACA Tsp45I 8 GTGAC ^GT[GC]AC 722 GTCAC TspRI 724 CACTG CA[GC]TG[ACGT][ACGT]^ 1347 CAGTG XhoII 197 GGATCT [GA]^GATC[CT] 549 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AlwI BbvI BsaJI BsmAI BspMI BsrI BssKI BstNI DpnII EcoRII EcoT14I Fnu4HI HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PstI RsaI Sau3AI ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TthHB8I