(Circular) (Six-base) MAPSORT of: pFN21AE1348.seq Check: 1498 from: 1 to: 6121 pFN21AE1348 6121 bp With 182 enzymes: SgfI * December 17, 2009 17:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3239 4184 5568 5836 1632 Size: 1607 945 1384 268 1917 Fragments arranged by size: 1917 1607 1384 945 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 3288 5020 5997 1203 Size: 2085 1732 977 1327 Fragments arranged by size: 2085 1732 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 2399 3864 5436 2399 Size: 1465 1572 3084 Fragments arranged by size: 3084 1572 1465 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 3198 3430 3656 3808 5917 1493 Size: 1705 232 226 152 2109 1697 Fragments arranged by size: 2109 1705 1697 232 226 152 AseI AT'TA_AT Cuts at: 160 1032 2742 4675 160 Size: 872 1710 1933 1606 Fragments arranged by size: 1933 1710 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BamHI G'GATC_C Cuts at: 2554 3451 3818 2554 Size: 897 367 4857 Fragments arranged by size: 4857 897 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2360 3442 4897 618 Size: 325 217 627 573 1082 1455 1842 Fragments arranged by size: 1842 1455 1082 627 573 325 217 BanII G_rGCy'C Cuts at: 1391 2031 3440 1391 Size: 640 1409 4072 Fragments arranged by size: 4072 1409 640 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2117 928 Size: 971 218 4932 Fragments arranged by size: 4932 971 218 BbvCI CC'TCA_GC Cuts at: 2325 2325 Size: 6121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3387 3421 4312 4346 1903 Size: 34 1450 34 891 34 3678 Fragments arranged by size: 3678 1450 891 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 4182 5338 1790 Size: 2392 1156 2573 Fragments arranged by size: 2573 2392 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 3852 4810 Size: 416 53 83 399 24 1853 582 958 Cuts at: 4810 442 Size: 1753 Fragments arranged by size: 1853 1753 958 582 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2895 4781 1239 Size: 1656 1886 2579 Fragments arranged by size: 2579 1886 1656 Bpu10I CC'TnA_GC Cuts at: 2325 2325 Size: 6121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2088 3007 4784 882 Size: 617 589 919 1777 2219 Fragments arranged by size: 2219 1777 919 617 589 BsaAI yAC'GTr Cuts at: 493 1204 2280 3912 493 Size: 711 1076 1632 2702 Fragments arranged by size: 2702 1632 1076 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2016 4554 4952 5226 5373 5784 2016 Size: 2538 398 274 147 411 2353 Fragments arranged by size: 2538 2353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5896 Fragments arranged by size: 5896 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6121 BseYI C'CCAG_C Cuts at: 2036 5324 5820 2036 Size: 3288 496 2337 Fragments arranged by size: 3288 2337 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2926 1163 Size: 573 1190 4358 Fragments arranged by size: 4358 1190 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4182 4267 5338 4182 Size: 85 1071 4965 Fragments arranged by size: 4965 1071 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 3440 4182 4267 5338 1391 Size: 399 241 1409 742 85 1071 2174 Fragments arranged by size: 2174 1409 1071 742 399 241 85 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2106 2811 3157 3476 844 Size: 1262 705 346 319 3489 Fragments arranged by size: 3489 1262 705 346 319 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2969 3021 96 Size: 2873 52 3196 Fragments arranged by size: 3196 2873 52 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 2370 4925 2370 Size: 2555 3566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6121 BstYI r'GATC_y Cuts at: 1972 2202 2554 3451 3818 4203 4220 5672 5758 Size: 230 352 897 367 385 17 1452 86 Cuts at: 5758 5770 6116 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 897 385 367 352 346 230 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4630 Fragments arranged by size: 4630 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2017 487 Size: 683 56 791 4591 Fragments arranged by size: 4591 791 683 56 BtsI GCAGTG_nn' Cuts at: 2728 3637 4431 4451 2728 Size: 909 794 20 4398 Fragments arranged by size: 4398 909 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 2158 3425 2158 Size: 1267 4854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2336 5128 809 Size: 507 1020 2792 1802 Fragments arranged by size: 2792 1802 1020 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3568 4457 6086 Size: 54 1425 50 275 18 1738 889 1629 Cuts at: 6086 8 Size: 43 Fragments arranged by size: 1738 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3215 4051 1353 Size: 1862 836 3423 Fragments arranged by size: 3423 1862 836 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2895 3239 4184 4781 5568 5836 1239 Size: 393 1263 344 945 597 787 268 1524 Fragments arranged by size: 1524 1263 945 787 597 393 344 268 EcoO109I rG'GnC_Cy Cuts at: 2270 2270 Size: 6121 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2289 2289 Size: 6121 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2744 2776 4762 4794 4836 Size: 32 135 32 1172 32 1986 32 42 Cuts at: 4836 4868 1373 Size: 32 2626 Fragments arranged by size: 2626 1986 1172 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3172 3465 3711 669 Size: 988 281 1234 293 246 3079 Fragments arranged by size: 3079 1234 988 293 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 2148 3720 2148 Size: 1572 4549 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2071 2368 2871 5235 5419 1305 Size: 766 297 503 2364 184 2007 Fragments arranged by size: 2364 2007 766 503 297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4299 Fragments arranged by size: 4299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4079 4438 4597 518 Size: 648 2913 359 159 2042 Fragments arranged by size: 2913 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2435 3537 4214 5362 5607 1606 Size: 829 1102 677 1148 245 2120 Fragments arranged by size: 2120 1148 1102 829 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4630 Fragments arranged by size: 4630 939 552 NdeI CA'TA_TG Cuts at: 387 2977 387 Size: 2590 3531 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3292 3402 3479 5024 1132 Size: 97 2063 110 77 1545 2229 Fragments arranged by size: 2229 2063 1545 110 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 3288 5020 3288 Size: 1732 4389 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2270 2270 Size: 6121 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 2640 3691 2640 Size: 1051 5070 PstI C_TGCA'G Cuts at: 830 2352 2629 3168 3473 830 Size: 1522 277 539 305 3478 Fragments arranged by size: 3478 1522 539 305 277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 2435 2435 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2348 2548 2625 3164 3469 4604 5285 Size: 219 1303 200 77 539 305 1135 681 Cuts at: 5285 5476 826 Size: 191 1471 Fragments arranged by size: 1471 1303 1135 681 539 305 219 200 191 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2280 3912 493 Size: 1787 1632 2702 Fragments arranged by size: 2702 1787 1632 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3402 3479 1132 Size: 2270 77 3774 Fragments arranged by size: 3774 2270 77 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 2900 1517 Size: 1383 4738 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 2895 3325 3560 513 Size: 552 939 297 594 430 235 3074 Fragments arranged by size: 3074 939 594 552 430 297 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4148 4165 4318 4503 4948 325 Size: 1255 2568 17 153 185 445 1498 Fragments arranged by size: 2568 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2969 3021 4367 Size: 274 80 33 51 1443 992 52 1346 Cuts at: 4367 6079 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1346 992 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2856 2888 3256 3288 6081 6113 2856 Size: 32 368 32 2793 32 2864 Fragments arranged by size: 2864 2793 368 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 3198 4250 3198 Size: 1052 5069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI