Restriction map for
pFN21AE1443
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BciVI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsgI BsiWI BsmFI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI HphI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NdeI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1063 GTCTAC GT^[AC][GT]AC
AccII 111 CGCG CG^CG
Aor51HI 131 AGCGCT AGC^GCT
BbeI 1135 GGCGCC GGCGC^C
BbrPI 1071 CACGTG CAC^GTG
BbsI 1099 GAAGAC GAAGAC(2/6)
BbvCI 844 GCTGAGG CCTCAGC(-5/-2)
BlpI 1286 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1286 GCTCAGC GC^T[ACGT]AGC
BpuAI 1099 GAAGAC GAAGAC(2/6)
BsaAI 1071 CACGTG [CT]AC^GT[GA]
BsiEI 199 CGGCCG CG[GA][CT]^CG
BsmBI 825 CGTCTC CGTCTC(1/5)
BspDI 691 ATCGAT AT^CGAT
BspMI 433 ACCTGC ACCTGC(4/8)
BstAPI 1369 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 111 CGCG CG^CG
CelII 1286 GCTCAGC GC^T[ACGT]AGC
ClaI 691 ATCGAT AT^CGAT
DraII 356 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 797 CCATGG C^C[GA][CT]GG
EagI 199 CGGCCG C^GGCCG
EclXI 199 CGGCCG C^GGCCG
Eco47III 131 AGCGCT AGC^GCT
Eco52I 199 CGGCCG C^GGCCG
EcoO109I 356 AGGACCT [GA]G^G[ACGT]CC[CT]
KasI 1135 GGCGCC G^GCGCC
MaeIII 725 GTCAC ^GT[ACGT]AC
MslI 334 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 111 CGCG CG^CG
NarI 1135 GGCGCC GG^CGCC
NcoI 797 CCATGG C^CATGG
NheI 1146 GCTAGC G^CTAGC
NotI 198 GCGGCCGC GC^GGCCGC
NspI 993 GCATGC [GA]CATG^[CT]
PmaCI 1071 CACGTG CAC^GTG
PmlI 1071 CACGTG CAC^GTG
PpuMI 356 AGGACCT [GA]G^G[AT]CC[CT]
SapI 736 GCTCTTC GCTCTTC(1/4)
SfoI 1135 GGCGCC GGC^GCC
SphI 993 GCATGC GCATG^C
Tsp45I 725 GTCAC ^GT[GC]AC
Tsp509I 1263 AATT ^AATT
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 823 GGCGTC G[GA]^CG[CT]C
1135 GGCGCC
BalI 209 TGGCCA TGG^CCA
800 TGGCCA
BcgI 693 CGATGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1018 CGAGCCCTGTGC
BglII 585 AGATCT A^GATCT
758 AGATCT
BpmI 78 CTCCAG CTGGAG(16/14)
952 CTGGAG
BsaHI 823 GGCGTC G[GA]^CG[CT]C
1135 GGCGCC
BsaI 1228 GAGACC GGTCTC(1/5)
864 GGTCTC
BsrI 840 ACTGG ACTGG(1/-1)
722 CCAGT
EarI 737 CTCTTC CTCTTC(1/4)
1350 GAAGAG
EcoNI 207 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1080 CCTCTGCCAGG
HgaI 824 GCGTC GACGC(5/10)
987 GCGTC
Hin1I 823 GGCGTC G[GA]^CG[CT]C
1135 GGCGCC
Ksp632I 737 CTCTTC CTCTTC(1/4)
1350 GAAGAG
MluNI 209 TGGCCA TGG^CCA
800 TGGCCA
MscI 209 TGGCCA TGG^CCA
800 TGGCCA
StuI 379 AGGCCT AGG^CCT
1179 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BglI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MaeI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI TspRI TthHB8I XhoII
XmaCI XmaI