(Circular) (Six-base) MAPSORT of: pFN21AE1443.seq Check: 5881 from: 1 to: 6103 pFN21AE1443 6103 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3069 3446 1937 Size: 1132 377 4594 Fragments arranged by size: 4594 1132 377 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2768 3130 4166 5550 5818 1632 Size: 1136 362 1036 1384 268 1917 Fragments arranged by size: 1917 1384 1136 1036 362 268 AfeI AGC'GCT Cuts at: 2138 2138 Size: 6103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 3846 5418 3846 Size: 1572 4531 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 3412 3638 3790 5899 1493 Size: 1919 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2820 2935 2966 3136 3199 3428 1870 Size: 73 877 115 31 170 63 229 4545 Fragments arranged by size: 4545 877 229 170 115 73 63 31 BamHI G'GATC_C Cuts at: 2378 2881 2917 3433 3800 2378 Size: 503 36 516 367 4681 Fragments arranged by size: 4681 516 503 367 36 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 3140 3203 3283 3424 Size: 325 217 627 508 845 63 80 141 Cuts at: 3424 4879 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 845 627 508 325 217 141 80 63 BanII G_rGCy'C Cuts at: 1391 2063 2524 2967 3028 3422 1391 Size: 672 461 443 61 394 4072 Fragments arranged by size: 4072 672 461 443 394 61 BbeI G_GCGC'C Cuts at: 3144 3144 Size: 6103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 928 Size: 971 1212 3920 Fragments arranged by size: 3920 1212 971 BbvCI CC'TCA_GC Cuts at: 2850 2850 Size: 6103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2687 2721 3012 3046 4294 4328 1903 Size: 34 750 34 291 34 1248 34 3678 Fragments arranged by size: 3678 1248 750 291 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 3172 3302 Size: 107 122 71 631 605 386 1114 130 Cuts at: 3302 4714 136 Size: 1412 1525 Fragments arranged by size: 1525 1412 1114 631 605 386 130 122 107 71 BglII A'GATC_T Cuts at: 2590 2763 6098 2590 Size: 173 3335 2595 Fragments arranged by size: 3335 2595 173 BlpI GC'TnA_GC Cuts at: 3292 3520 3292 Size: 228 5875 Bme1580I G_kGCm'C Cuts at: 1790 4164 5320 1790 Size: 2374 1156 2573 Fragments arranged by size: 2573 2374 1156 BmgBI CAC'GTC Cuts at: 1318 3109 1318 Size: 1791 4312 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3834 4792 442 Size: 416 53 83 399 24 2417 958 1753 Fragments arranged by size: 2417 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3155 1056 Size: 2099 4004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2978 4763 1239 Size: 829 910 1785 2579 Fragments arranged by size: 2579 1785 910 829 Bpu10I CC'TnA_GC Cuts at: 2850 3188 2850 Size: 338 5765 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 2315 2417 2510 4228 5093 5391 2294 Size: 21 102 93 1718 865 298 3006 Fragments arranged by size: 3006 1718 865 298 102 93 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2875 3227 4766 882 Size: 617 1376 352 1539 2219 Fragments arranged by size: 2219 1539 1376 617 352 BsaAI yAC'GTr Cuts at: 493 1204 3078 3894 493 Size: 711 1874 816 2702 Fragments arranged by size: 2702 1874 816 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2829 3141 3910 4292 Size: 53 83 186 936 1296 312 769 382 Cuts at: 4292 4927 275 Size: 635 1451 Fragments arranged by size: 1451 1296 936 769 635 382 312 186 83 53 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5878 Fragments arranged by size: 5878 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 2867 2975 3110 1186 Size: 985 696 108 135 4179 Fragments arranged by size: 4179 985 696 135 108 BseYI C'CCAG_C Cuts at: 2400 2656 3332 3337 5306 5802 2400 Size: 256 676 5 1969 496 2701 Fragments arranged by size: 2701 1969 676 496 256 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2207 3553 4314 5342 1540 Size: 275 186 206 1346 761 1028 2301 Fragments arranged by size: 2301 1346 1028 761 275 206 186 BsiHKAI G_wGCw'C Cuts at: 3083 3194 3213 4164 4249 5320 3083 Size: 111 19 951 85 1071 3866 Fragments arranged by size: 3866 1071 951 111 85 19 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 BsmBI CGTCTCn'nnnn_ Cuts at: 2836 2836 Size: 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2524 2967 3028 3083 3194 3213 Size: 399 273 461 443 61 55 111 19 Cuts at: 3213 3422 4164 4249 5320 1391 Size: 209 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 461 443 399 273 209 111 85 61 55 19 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 3992 5722 3992 Size: 1730 4373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2447 3458 844 Size: 1603 1011 3489 Fragments arranged by size: 3489 1603 1011 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 2174 2292 3377 4747 4934 1700 Size: 110 229 135 118 1085 1370 187 2869 Fragments arranged by size: 2869 1370 1085 229 187 135 118 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 3380 794 Size: 2586 3517 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstXI CCAn_nnnn'nTGG Cuts at: 2668 2734 2794 2815 2668 Size: 66 60 21 5956 Fragments arranged by size: 5956 66 60 21 BstYI r'GATC_y Cuts at: 1972 2378 2590 2763 2881 2917 3433 3800 4185 Size: 406 212 173 118 36 516 367 385 Cuts at: 4185 4202 5654 5740 5752 6098 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 516 406 385 367 346 212 173 118 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2802 513 Size: 552 939 798 3814 Fragments arranged by size: 3814 939 798 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2935 3211 3253 3619 4413 4433 2935 Size: 276 42 366 794 20 4605 Fragments arranged by size: 4605 794 366 276 42 20 ClaI AT'CG_AT Cuts at: 1554 2697 1554 Size: 1143 4960 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2204 2214 Size: 54 1425 50 275 18 207 167 10 Cuts at: 2214 2805 3550 4439 6068 8 Size: 591 745 889 1629 43 Fragments arranged by size: 1629 1425 889 745 591 275 207 167 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2204 3550 1537 Size: 275 392 1346 4090 Fragments arranged by size: 4090 1346 392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2748 3350 4033 1353 Size: 1395 602 683 3423 Fragments arranged by size: 3423 1395 683 602 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2134 4690 5064 5210 387 Size: 1064 138 545 2556 374 146 1280 Fragments arranged by size: 2556 1280 1064 545 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2768 2978 3130 4166 4763 5550 Size: 393 436 700 210 152 1036 597 787 Cuts at: 5550 5818 1239 Size: 268 1524 Fragments arranged by size: 1524 1036 787 700 597 436 393 268 210 152 EcoNI CCTnn'n_nnAGG Cuts at: 2216 3089 2216 Size: 873 5230 EcoO109I rG'GnC_Cy Cuts at: 2362 2362 Size: 6103 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 2140 2175 3144 5250 1678 Size: 462 35 969 2106 2531 Fragments arranged by size: 2531 2106 969 462 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4744 4776 4818 4850 1373 Size: 32 135 32 3172 32 42 32 2626 Fragments arranged by size: 3172 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3447 3693 669 Size: 988 281 1509 246 3079 Fragments arranged by size: 3079 1509 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KasI G'GCGC_C Cuts at: 3140 3140 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2349 5217 5401 1305 Size: 1044 2868 184 2007 Fragments arranged by size: 2868 2007 1044 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 2807 10 Size: 54 1425 343 384 591 3306 Fragments arranged by size: 3306 1425 591 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 4061 4420 4579 518 Size: 648 1177 1718 359 159 2042 Fragments arranged by size: 2042 1718 1177 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2110 2404 2649 2956 3061 3519 4196 Size: 418 86 294 245 307 105 458 677 Cuts at: 4196 5344 5589 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 458 418 307 294 245 245 105 86 NaeI GCC'GGC Cuts at: 2041 2176 2294 2041 Size: 135 118 5850 Fragments arranged by size: 5850 135 118 NarI GG'CG_CC Cuts at: 3141 3141 Size: 6103 NcoI C'CATG_G Cuts at: 513 1065 2004 2802 513 Size: 552 939 798 3814 Fragments arranged by size: 3814 939 798 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NgoMIV G'CCGG_C Cuts at: 2039 2174 2292 2039 Size: 135 118 5850 Fragments arranged by size: 5850 135 118 NheI G'CTAG_C Cuts at: 1052 3151 1052 Size: 2099 4004 NotI GC'GGCC_GC Cuts at: 2204 3550 2204 Size: 1346 4757 NspI r_CATG'y Cuts at: 1132 1229 3002 3461 5006 1132 Size: 97 1773 459 1545 2229 Fragments arranged by size: 2229 1773 1545 459 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6103 PfoI T'CCnGG_A Cuts at: 2544 2544 Size: 6103 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PmlI CAC'GTG Cuts at: 3078 3078 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 6103 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PstI C_TGCA'G Cuts at: 830 2023 2029 2637 2930 3060 3129 3455 830 Size: 1193 6 608 293 130 69 326 3478 Fragments arranged by size: 3478 1193 608 326 293 130 69 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2024 2110 2404 2649 2956 2024 Size: 86 294 245 307 5171 Fragments arranged by size: 5171 307 294 245 86 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SapI GCTCTTCn'nnn_ Cuts at: 2748 2748 Size: 6103 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 2019 2025 2633 2926 3056 3125 3451 Size: 219 974 6 608 293 130 69 326 Cuts at: 3451 4586 5267 5458 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 974 681 608 326 293 219 191 130 69 6 SfoI GGC'GCC Cuts at: 3142 3142 Size: 6103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 2822 2968 3138 3201 3430 1872 Size: 950 146 170 63 229 4545 Fragments arranged by size: 4545 950 229 170 146 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 2330 2432 2525 4207 5108 Size: 197 926 366 21 102 93 1682 901 Cuts at: 5108 5370 820 Size: 262 1553 Fragments arranged by size: 1682 1553 926 901 366 262 197 102 93 21 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3002 3461 1132 Size: 1870 459 3774 Fragments arranged by size: 3774 1870 459 SrfI GCCC'GGGC Cuts at: 2822 2822 Size: 6103 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 2386 3186 1517 Size: 869 800 4434 Fragments arranged by size: 4434 869 800 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 2755 2802 3542 513 Size: 552 939 213 538 47 740 3074 Fragments arranged by size: 3074 939 740 552 538 213 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2619 3346 4130 4147 4300 4485 4930 Size: 1255 1039 727 784 17 153 185 445 Cuts at: 4930 325 Size: 1498 Fragments arranged by size: 1498 1255 1039 784 727 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 4349 6061 Size: 274 80 33 51 1443 102 2270 1712 Cuts at: 6061 96 Size: 138 Fragments arranged by size: 2270 1712 1443 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3030 3062 6063 6095 3030 Size: 32 3001 32 3038 Fragments arranged by size: 3038 3001 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 2820 2966 3136 3199 3428 1870 Size: 950 146 170 63 229 4545 Fragments arranged by size: 4545 950 229 170 146 63 XmnI GAAnn'nnTTC Cuts at: 4232 4232 Size: 6103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI XcmI