(Linear) (Six-base) MAPSORT of: KIEE1443.seq Check: 3641 from: 1 to: 1398 KIEE1443.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AccI GT'mk_AC Cuts at: 0 1064 1398 Size: 1064 334 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 763 1125 1398 Size: 763 362 273 Fragments arranged by size: 763 362 273 AfeI AGC'GCT Cuts at: 0 133 1398 Size: 133 1265 AvaI C'yCGr_G Cuts at: 0 815 930 961 1131 1194 1398 Size: 815 115 31 170 63 204 Fragments arranged by size: 815 204 170 115 63 31 BamHI G'GATC_C Cuts at: 0 373 876 912 1398 Size: 373 503 36 486 Fragments arranged by size: 503 486 373 36 BanI G'GyrC_C Cuts at: 0 290 1135 1198 1278 1398 Size: 290 845 63 80 120 Fragments arranged by size: 845 290 120 80 63 BanII G_rGCy'C Cuts at: 0 58 519 962 1023 1398 Size: 58 461 443 61 375 Fragments arranged by size: 461 443 375 61 58 BbeI G_GCGC'C Cuts at: 0 1139 1398 Size: 1139 259 BbsI GAAGACnn'nnnn_ Cuts at: 0 1106 1398 Size: 1106 292 BbvCI CC'TCA_GC Cuts at: 0 845 1398 Size: 845 553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 682 716 1007 1041 1398 Size: 682 34 291 34 357 Fragments arranged by size: 682 357 291 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 53 1167 1297 1398 Size: 53 1114 130 101 Fragments arranged by size: 1114 130 101 53 BglII A'GATC_T Cuts at: 0 585 758 1398 Size: 585 173 640 Fragments arranged by size: 640 585 173 BlpI GC'TnA_GC Cuts at: 0 1287 1398 Size: 1287 111 BmgBI CAC'GTC Cuts at: 0 1104 1398 Size: 1104 294 BmtI G_CTAG'C Cuts at: 0 1150 1398 Size: 1150 248 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 973 1398 Size: 63 910 425 Fragments arranged by size: 910 425 63 Bpu10I CC'TnA_GC Cuts at: 0 845 1183 1398 Size: 845 338 215 Fragments arranged by size: 845 338 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 310 412 505 1398 Size: 289 21 102 93 893 Fragments arranged by size: 893 289 102 93 21 BsaI GGTCTCn'nnnn_ Cuts at: 0 870 1222 1398 Size: 870 352 176 Fragments arranged by size: 870 352 176 BsaAI yAC'GTr Cuts at: 0 1073 1398 Size: 1073 325 BsaHI Gr'CG_yC Cuts at: 0 824 1136 1398 Size: 824 312 262 Fragments arranged by size: 824 312 262 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 166 862 970 1105 1398 Size: 166 696 108 135 293 Fragments arranged by size: 696 293 166 135 108 BseYI C'CCAG_C Cuts at: 0 395 651 1327 1332 1398 Size: 395 256 676 5 66 Fragments arranged by size: 676 395 256 66 5 BsiEI CG_ry'CG Cuts at: 0 202 1398 Size: 202 1196 BsiHKAI G_wGCw'C Cuts at: 0 1078 1189 1208 1398 Size: 1078 111 19 190 Fragments arranged by size: 1078 190 111 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 831 1398 Size: 831 567 Bsp1286I G_dGCh'C Cuts at: 0 58 519 962 1023 1078 1189 1208 1398 Size: 58 461 443 61 55 111 19 190 Fragments arranged by size: 461 443 190 111 61 58 55 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 442 1398 Size: 442 956 BsrFI r'CCGG_y Cuts at: 0 34 169 287 1372 1398 Size: 34 135 118 1085 26 Fragments arranged by size: 1085 135 118 34 26 BstAPI GCAn_nnn'nTGC Cuts at: 0 1375 1398 Size: 1375 23 BstXI CCAn_nnnn'nTGG Cuts at: 0 663 729 789 810 1398 Size: 663 66 60 21 588 Fragments arranged by size: 663 588 66 60 21 BstYI r'GATC_y Cuts at: 0 373 585 758 876 912 1398 Size: 373 212 173 118 36 486 Fragments arranged by size: 486 373 212 173 118 36 BtgI C'CryG_G Cuts at: 0 797 1398 Size: 797 601 BtsI GCAGTG_nn' Cuts at: 0 930 1206 1248 1398 Size: 930 276 42 150 Fragments arranged by size: 930 276 150 42 ClaI AT'CG_AT Cuts at: 0 692 1398 Size: 692 706 EaeI y'GGCC_r Cuts at: 0 32 199 209 800 1398 Size: 32 167 10 591 598 Fragments arranged by size: 598 591 167 32 10 EagI C'GGCC_G Cuts at: 0 199 1398 Size: 199 1199 EarI CTCTTCn'nnn_ Cuts at: 0 743 1345 1398 Size: 743 602 53 Fragments arranged by size: 743 602 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 129 1398 Size: 129 1269 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 763 973 1125 1398 Size: 63 700 210 152 273 Fragments arranged by size: 700 273 210 152 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 211 1084 1398 Size: 211 873 314 Fragments arranged by size: 873 314 211 EcoO109I rG'GnC_Cy Cuts at: 0 357 1398 Size: 357 1041 HaeII r_GCGC'y Cuts at: 0 135 170 1139 1398 Size: 135 35 969 259 Fragments arranged by size: 969 259 135 35 KasI G'GCGC_C Cuts at: 0 1135 1398 Size: 1135 263 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1398 Size: 344 1054 MscI TGG'CCA Cuts at: 0 211 802 1398 Size: 211 591 596 Fragments arranged by size: 596 591 211 MslI CAynn'nnrTG Cuts at: 0 338 1398 Size: 338 1060 MspA1I CmG'CkG Cuts at: 0 19 105 399 644 951 1056 1398 Size: 19 86 294 245 307 105 342 Fragments arranged by size: 342 307 294 245 105 86 19 NaeI GCC'GGC Cuts at: 0 36 171 289 1398 Size: 36 135 118 1109 Fragments arranged by size: 1109 135 118 36 NarI GG'CG_CC Cuts at: 0 1136 1398 Size: 1136 262 NcoI C'CATG_G Cuts at: 0 797 1398 Size: 797 601 NgoMIV G'CCGG_C Cuts at: 0 34 169 287 1398 Size: 34 135 118 1111 Fragments arranged by size: 1111 135 118 34 NheI G'CTAG_C Cuts at: 0 1146 1398 Size: 1146 252 NotI GC'GGCC_GC Cuts at: 0 199 1398 Size: 199 1199 NspI r_CATG'y Cuts at: 0 997 1398 Size: 997 401 PfoI T'CCnGG_A Cuts at: 0 539 1398 Size: 539 859 PmlI CAC'GTG Cuts at: 0 1073 1398 Size: 1073 325 PpuMI rG'GwC_Cy Cuts at: 0 357 1398 Size: 357 1041 PstI C_TGCA'G Cuts at: 0 18 24 632 925 1055 1124 1398 Size: 18 6 608 293 130 69 274 Fragments arranged by size: 608 293 274 130 69 18 6 PvuII CAG'CTG Cuts at: 0 19 105 399 644 951 1398 Size: 19 86 294 245 307 447 Fragments arranged by size: 447 307 294 245 86 19 SapI GCTCTTCn'nnn_ Cuts at: 0 743 1398 Size: 743 655 SfcI C'TryA_G Cuts at: 0 14 20 628 921 1051 1120 1398 Size: 14 6 608 293 130 69 278 Fragments arranged by size: 608 293 278 130 69 14 6 SfoI GGC'GCC Cuts at: 0 1137 1398 Size: 1137 261 SmaI CCC'GGG Cuts at: 0 817 963 1133 1196 1398 Size: 817 146 170 63 202 Fragments arranged by size: 817 202 170 146 63 SmlI C'TyrA_G Cuts at: 0 304 325 427 520 1398 Size: 304 21 102 93 878 Fragments arranged by size: 878 304 102 93 21 SphI G_CATG'C Cuts at: 0 997 1398 Size: 997 401 SrfI GCCC'GGGC Cuts at: 0 817 1398 Size: 817 581 StuI AGG'CCT Cuts at: 0 381 1181 1398 Size: 381 800 217 Fragments arranged by size: 800 381 217 StyI C'CwwG_G Cuts at: 0 212 750 797 1398 Size: 212 538 47 601 Fragments arranged by size: 601 538 212 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 614 1341 1398 Size: 614 727 57 Fragments arranged by size: 727 614 57 TatI w'GTAC_w Cuts at: 0 74 1398 Size: 74 1324 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1025 1057 1398 Size: 1025 32 341 Fragments arranged by size: 1025 341 32 XmaI C'CCGG_G Cuts at: 0 815 961 1131 1194 1398 Size: 815 146 170 63 204 Fragments arranged by size: 815 204 170 146 63 Enzymes that do cut: AccI AcuI AfeI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI BtgI BtsI ClaI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI PfoI PmlI PpuMI PstI PvuII SapI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI Bme1580I BmrI BplI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NdeI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI