Restriction map for
pFN21AB6657
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AciI
AcyI AfaI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfaI BglI
BlnI BmyI BpmI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MamI MluI
MroI MspI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AclI 929 AACGTT AA^CGTT
AcsI 607 AAATTT [GA]^AATT[CT]
AflII 868 CTTAAG C^TTAAG
AflIII 147 ACATGT A^C[GA][CT]GT
ApoI 607 AAATTT [GA]^AATT[CT]
BalI 762 TGGCCA TGG^CCA
BamHI 972 GGATCC G^GATCC
BciVI 1110 GGATAC GTATCC(6/5)
BfrI 868 CTTAAG C^TTAAG
BglII 641 AGATCT A^GATCT
BlpI 4 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4 GCTCAGC GC^T[ACGT]AGC
BsaAI 253 TACGTG [CT]AC^GT[GA]
BsaI 1049 GGTCTC GGTCTC(1/5)
BsgI 269 CTGCAC GTGCAG(16/14)
BsmAI 1050 GTCTC GTCTC(1/5)
BsmI 188 GAATGC GAATGC(1/-1)
BspHI 1152 TCATGA T^CATGA
BspLU11I 147 ACATGT A^CATGT
BsrDI 285 GCAATG GCAATG(2/0)
Bst1107I 264 GTATAC GTA^TAC
BstZ17I 264 GTATAC GTA^TAC
CelII 4 GCTCAGC GC^T[ACGT]AGC
EaeI 762 TGGCCA [CT]^GGCC[GA]
EcoNI 165 CCTTATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 288 ATGCAT ATGCA^T
HincII 873 GTCAAC GT[CT]^[GA]AC
HindII 873 GTCAAC GT[CT]^[GA]AC
MfeI 404 CAATTG C^AATTG
MluNI 762 TGGCCA TGG^CCA
MscI 762 TGGCCA TGG^CCA
MspA1I 1069 CAGCTG C[AC]G^C[GT]G
MunI 404 CAATTG C^AATTG
NsiI 288 ATGCAT ATGCA^T
Psp1406I 929 AACGTT AA^CGTT
PstI 813 CTGCAG CTGCA^G
PvuII 1069 CAGCTG CAG^CTG
RcaI 1152 TCATGA T^CATGA
SmlI 868 CTTAAG C^T[CT][GA]AG
TaqI 696 TCGA T^CGA
TfiI 577 GATTC G^A[AT]TC
TthHB8I 696 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 377 GGTCC G^G[AT]CC
828 GGACC
BbsI 714 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BpuAI 714 GAAGAC GAAGAC(2/6)
1156 GAAGAC
BssKI 440 CCTGG ^CC[ACGT]GG
623 CCTGG
BstF5I 420 GGATG GGATG(2/0)
849 GGATG
BstNI 440 CCTGG CC^[AT]GG
623 CCTGG
EcoRII 440 CCTGG ^CC[AT]GG
623 CCTGG
EcoT14I 194 CCTTGG C^C[AT][AT]GG
380 CCTTGG
FokI 420 GGATG GGATG(9/13)
849 GGATG
MvaI 440 CCTGG CC^[AT]GG
623 CCTGG
NspI 147 ACATGT [GA]CATG^[CT]
290 GCATGT
ScrFI 440 CCTGG CC^[ACGT]GG
623 CCTGG
StyI 194 CCTTGG C^C[AT][AT]GG
380 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccI AluI AlwI AlwNI BbvI
BsaJI BseRI BsiYI BslI BsmFI
BsrI BstYI Cfr13I DdeI DpnII
Fnu4HI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII