(Circular) (Six-base) MAPSORT of: pFN21AB6657.seq Check: 4037 from: 1 to: 5974 pFN21AB6657 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2270 2654 3180 3317 1937 Size: 333 384 526 137 4594 Fragments arranged by size: 4594 526 384 333 137 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 2935 4101 4474 1563 Size: 1372 1166 373 3063 Fragments arranged by size: 3063 1372 1166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2829 4037 5421 5689 1632 Size: 1197 1208 1384 268 1917 Fragments arranged by size: 1917 1384 1208 1197 268 AflII C'TTAA_G Cuts at: 820 1017 2873 820 Size: 197 1856 3921 Fragments arranged by size: 3921 1856 197 AflIII A'CryG_T Cuts at: 1203 2152 4873 5850 1203 Size: 949 2721 977 1327 Fragments arranged by size: 2721 1327 977 949 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2188 2807 3076 3717 5289 2188 Size: 619 269 641 1572 2873 Fragments arranged by size: 2873 1572 641 619 269 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2612 3283 3509 3661 5770 1493 Size: 1119 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 1119 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BamHI G'GATC_C Cuts at: 2977 3304 3671 2977 Size: 327 367 5280 Fragments arranged by size: 5280 367 327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3293 1391 Size: 1902 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2726 3168 928 Size: 971 827 442 3734 Fragments arranged by size: 3734 971 827 442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3109 3866 5076 3109 Size: 757 1210 4007 Fragments arranged by size: 4007 1210 757 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BfrBI ATG'CAT Cuts at: 2295 2295 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2646 5969 2646 Size: 3323 2651 BlpI GC'TnA_GC Cuts at: 2010 3391 2010 Size: 1381 4593 Bme1580I G_kGCm'C Cuts at: 1790 4035 5191 1790 Size: 2245 1156 2573 Fragments arranged by size: 2573 2245 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3705 4663 442 Size: 416 53 83 399 24 2288 958 1753 Fragments arranged by size: 2288 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2245 2245 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4634 1239 Size: 3395 2579 Bpu10I CC'TnA_GC Cuts at: 2602 2602 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4099 4964 5262 4099 Size: 865 298 4811 Fragments arranged by size: 4811 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3060 4637 882 Size: 617 1561 1577 2219 Fragments arranged by size: 2219 1577 1561 617 BsaAI yAC'GTr Cuts at: 493 1204 2260 3765 493 Size: 711 1056 1505 2702 Fragments arranged by size: 2702 1505 1056 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2731 2761 1178 Size: 30 165 30 1328 30 4391 Fragments arranged by size: 4391 1328 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 3126 3228 1186 Size: 1516 424 102 3932 Fragments arranged by size: 3932 1516 424 102 BseYI C'CCAG_C Cuts at: 2280 5177 5673 2280 Size: 2897 496 2581 Fragments arranged by size: 2897 2581 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2259 1163 Size: 573 523 4878 Fragments arranged by size: 4878 573 523 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4035 4120 5191 4035 Size: 85 1071 4818 Fragments arranged by size: 4818 1071 85 BsmI GAATG_Cn' Cuts at: 2199 3485 3578 2199 Size: 1286 93 4595 Fragments arranged by size: 4595 1286 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3293 4035 4120 5191 1391 Size: 399 1503 742 85 1071 2174 Fragments arranged by size: 2174 1503 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3157 3863 5593 3157 Size: 706 1730 3538 Fragments arranged by size: 3538 1730 706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2297 4469 4643 65 Size: 1183 1049 2172 174 1396 Fragments arranged by size: 2172 1396 1183 1049 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2343 2376 2646 2977 3086 3304 3671 4056 Size: 371 33 270 331 109 218 367 385 Cuts at: 4056 4073 5525 5611 5623 5969 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 371 367 346 331 270 218 109 86 33 17 12 BstZ17I GTA'TAC Cuts at: 2271 2271 Size: 5974 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2551 2626 2827 3490 4284 4304 2551 Size: 75 201 663 794 20 4221 Fragments arranged by size: 4221 794 663 201 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2767 3421 4310 Size: 54 1425 50 275 18 937 654 889 Cuts at: 4310 5939 8 Size: 1629 43 Fragments arranged by size: 1629 1425 937 889 654 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3904 1353 Size: 2551 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2829 4037 4634 5421 5689 1239 Size: 393 1197 1208 597 787 268 1524 Fragments arranged by size: 1524 1208 1197 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2174 2174 Size: 5974 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 2846 2878 4615 Size: 32 135 32 878 32 364 32 1737 Cuts at: 4615 4647 4689 4721 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1737 878 364 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3318 3564 669 Size: 988 281 942 438 246 3079 Fragments arranged by size: 3079 988 942 438 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 2409 3573 2409 Size: 1164 4810 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2769 10 Size: 54 1425 343 937 3215 Fragments arranged by size: 3215 1425 937 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2216 2424 3932 4291 4450 518 Size: 648 985 65 208 1508 359 159 2042 Fragments arranged by size: 2042 1508 985 648 359 208 159 65 MspA1I CmG'CkG Cuts at: 1606 3076 3390 4067 5215 5460 1606 Size: 1470 314 677 1148 245 2120 Fragments arranged by size: 2120 1470 1148 677 314 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NsiI A_TGCA'T Cuts at: 2297 2297 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2156 2299 3332 4877 1132 Size: 97 927 143 1033 1545 2229 Fragments arranged by size: 2229 1545 1033 927 143 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 2152 4873 2152 Size: 2721 3253 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2046 2078 2046 Size: 32 5942 PstI C_TGCA'G Cuts at: 830 2822 3326 830 Size: 1992 504 3478 Fragments arranged by size: 3478 1992 504 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 3076 3076 Size: 5974 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2557 2586 2655 2664 2818 3238 3322 Size: 219 1512 29 69 9 154 420 84 Cuts at: 3322 4457 5138 5329 826 Size: 1135 681 191 1471 Fragments arranged by size: 1512 1471 1135 681 420 219 191 154 84 69 29 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2873 4078 4979 5241 820 Size: 197 926 930 1205 901 262 1553 Fragments arranged by size: 1553 1205 930 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 2199 2385 3413 513 Size: 552 939 195 186 1028 3074 Fragments arranged by size: 3074 1028 939 552 195 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2437 3025 4001 4018 4171 4356 4801 Size: 1255 857 588 976 17 153 185 445 Cuts at: 4801 325 Size: 1498 Fragments arranged by size: 1498 1255 976 857 588 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4220 5932 96 Size: 274 80 33 51 1443 2243 1712 138 Fragments arranged by size: 2243 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2883 2915 5934 5966 2883 Size: 32 3019 32 2891 Fragments arranged by size: 3019 2891 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI