(Linear) (Six-base) MAPSORT of: KIBB6657.seq  Check: 9840  from: 1  to: 1269



KIBB6657.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:      0     265     649    1175    1269 
   Size:        265     384     526      94

  Fragments arranged by size: 

                 526     384     265      94
 
AclI AA'CG_TT

Cuts at:      0     930    1269 
   Size:        930     339
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1269 
   Size:        824     445
 
AflII C'TTAA_G

Cuts at:      0     868    1269 
   Size:        868     401
 
AflIII A'CryG_T

Cuts at:      0     147    1269 
   Size:        147    1122
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183     802    1071    1269 
   Size:        183     619     269     198

  Fragments arranged by size: 

                 619     269     198     183
 
ApoI r'AATT_y

Cuts at:      0     607    1269 
   Size:        607     662
 
BamHI G'GATC_C

Cuts at:      0     972    1269 
   Size:        972     297
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     721    1163    1269 
   Size:        721     442     106

  Fragments arranged by size: 

                 721     442     106
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1104    1269 
   Size:       1104     165
 
BfrBI ATG'CAT

Cuts at:      0     290    1269 
   Size:        290     979
 
BglII A'GATC_T

Cuts at:      0     641    1269 
   Size:        641     628
 
BlpI GC'TnA_GC

Cuts at:      0       5    1269 
   Size:          5    1264
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     240    1269 
   Size:        240    1029
 
Bpu10I CC'TnA_GC

Cuts at:      0     597    1269 
   Size:        597     672
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1055    1269 
   Size:       1055     214
 
BsaAI yAC'GTr

Cuts at:      0     255    1269 
   Size:        255    1014
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     726     756    1269 
   Size:        726      30     513

  Fragments arranged by size: 

                 726     513      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     697    1121    1223    1269 
   Size:        697     424     102      46

  Fragments arranged by size: 

                 697     424     102      46
 
BseYI C'CCAG_C

Cuts at:      0     275    1269 
   Size:        275     994
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1269 
   Size:        254    1015
 
BsmI GAATG_Cn'

Cuts at:      0     194    1269 
   Size:        194    1075
 
BspHI T'CATG_A

Cuts at:      0    1152    1269 
   Size:       1152     117
 
BsrDI GCAATG_nn'

Cuts at:      0     292    1269 
   Size:        292     977
 
BstYI r'GATC_y

Cuts at:      0     338     371     641     972    1081    1269 
   Size:        338      33     270     331     109     188

  Fragments arranged by size: 

                 338     331     270     188     109      33
 
BstZ17I GTA'TAC

Cuts at:      0     266    1269 
   Size:        266    1003
 
BtsI GCAGTG_nn'

Cuts at:      0     546     621     822    1269 
   Size:        546      75     201     447

  Fragments arranged by size: 

                 546     447     201      75
 
EaeI y'GGCC_r

Cuts at:      0     762    1269 
   Size:        762     507
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1269 
   Size:        824     445
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     169    1269 
   Size:        169    1100
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     841     873    1269 
   Size:        445      32     364      32     396

  Fragments arranged by size: 

                 445     396     364      32      32
 
HincII GTy'rAC

Cuts at:      0     875    1269 
   Size:        875     394
 
MfeI C'AATT_G

Cuts at:      0     404    1269 
   Size:        404     865
 
MscI TGG'CCA

Cuts at:      0     764    1269 
   Size:        764     505
 
MslI CAynn'nnrTG

Cuts at:      0     146     211     419    1269 
   Size:        146      65     208     850

  Fragments arranged by size: 

                 850     208     146      65
 
MspA1I CmG'CkG

Cuts at:      0    1071    1269 
   Size:       1071     198
 
NsiI A_TGCA'T

Cuts at:      0     292    1269 
   Size:        292     977
 
NspI r_CATG'y

Cuts at:      0     151     294    1269 
   Size:        151     143     975

  Fragments arranged by size: 

                 975     151     143
 
PciI A'CATG_T

Cuts at:      0     147    1269 
   Size:        147    1122
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      41      73    1269 
   Size:         41      32    1196

  Fragments arranged by size: 

                1196      41      32
 
PstI C_TGCA'G

Cuts at:      0     817    1269 
   Size:        817     452
 
PvuII CAG'CTG

Cuts at:      0    1071    1269 
   Size:       1071     198
 
SfcI C'TryA_G

Cuts at:      0     552     581     650     659     813    1233    1269 
   Size:        552      29      69       9     154     420      36

  Fragments arranged by size: 

                 552     420     154      69      36      29       9
 
SmlI C'TyrA_G

Cuts at:      0     868    1269 
   Size:        868     401
 
StyI C'CwwG_G

Cuts at:      0     194     380    1269 
   Size:        194     186     889

  Fragments arranged by size: 

                 889     194     186
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     432    1020    1269 
   Size:        432     588     249

  Fragments arranged by size: 

                 588     432     249
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     878     910    1269 
   Size:        878      32     359

  Fragments arranged by size: 

                 878     359      32
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII    AlwNI     ApoI    BamHI
     BbsI    BciVI    BfrBI    BglII     BlpI     BplI   Bpu10I     BsaI
    BsaAI    BsaXI    BseRI    BseYI     BsgI     BsmI    BspHI    BsrDI
    BstYI  BstZ17I     BtsI     EaeI  Eco57MI    EcoNI    Hin4I   HincII
     MfeI     MscI     MslI   MspA1I     NsiI     NspI     PciI     PsrI
     PstI    PvuII     SfcI     SmlI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI    BanII     BbeI    BbvCI     BcgI     BclI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI    BpuEI    BsaBI    BsaHI
    BsaWI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI