(Circular) (Six-base) MAPSORT of: pFN21AB6616.seq Check: 7230 from: 1 to: 6004 pFN21AB6616 6004 bp With 182 enzymes: SgfI * June 30, 2009 16:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2639 2639 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2973 4067 5451 5719 1632 Size: 1341 1094 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1094 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2073 4903 5880 1203 Size: 870 2830 977 1327 Fragments arranged by size: 2830 1327 977 870 AgeI A'CCGG_T Cuts at: 2561 2994 4835 2561 Size: 433 1841 3730 Fragments arranged by size: 3730 1841 433 AhdI GACnn_n'nnGTC Cuts at: 2719 4733 2719 Size: 2014 3990 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2029 3747 5319 2029 Size: 1718 1572 2714 Fragments arranged by size: 2714 1718 1572 ApaI G_GGCC'C Cuts at: 3235 3235 Size: 6004 ApaLI G'TGCA_C Cuts at: 2522 4061 5217 2522 Size: 1539 1156 3309 Fragments arranged by size: 3309 1539 1156 ApoI r'AATT_y Cuts at: 1493 2298 3313 3539 3691 5800 1493 Size: 805 1015 226 152 2109 1697 Fragments arranged by size: 2109 1697 1015 805 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2669 2827 3329 1870 Size: 73 726 158 502 4545 Fragments arranged by size: 4545 726 502 158 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2461 2494 2461 Size: 33 5971 BamHI G'GATC_C Cuts at: 2265 3334 3701 2265 Size: 1069 367 4568 Fragments arranged by size: 4568 1069 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 3016 3106 3325 4780 Size: 325 217 627 780 449 90 219 1455 Cuts at: 4780 618 Size: 1842 Fragments arranged by size: 1842 1455 780 627 449 325 219 217 90 BanII G_rGCy'C Cuts at: 1391 2651 2981 3235 3323 1391 Size: 1260 330 254 88 4072 Fragments arranged by size: 4072 1260 330 254 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3251 4615 136 Size: 107 122 71 631 605 1579 1364 1525 Fragments arranged by size: 1579 1525 1364 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3203 3421 3203 Size: 218 5786 Bme1580I G_kGCm'C Cuts at: 1790 2526 2967 3111 3235 4065 5221 1790 Size: 736 441 144 124 830 1156 2573 Fragments arranged by size: 2573 1156 830 736 441 144 124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 3735 4693 Size: 416 53 83 399 24 1027 1291 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1291 1027 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 4664 1239 Size: 1277 2148 2579 Fragments arranged by size: 2579 2148 1277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 2894 4129 4994 5292 2228 Size: 666 1235 865 298 2940 Fragments arranged by size: 2940 1235 865 666 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4667 882 Size: 617 3168 2219 Fragments arranged by size: 3168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2561 2931 2994 4437 4835 5109 5256 5667 2561 Size: 370 63 1443 398 274 147 411 2898 Fragments arranged by size: 2898 1443 411 398 370 274 147 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 2681 2711 1178 Size: 30 165 30 801 30 447 30 4471 Fragments arranged by size: 4471 801 447 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2326 3143 1186 Size: 1140 817 4047 Fragments arranged by size: 4047 1140 817 BseYI C'CCAG_C Cuts at: 2029 2167 2726 5207 5703 2029 Size: 138 559 2481 496 2330 Fragments arranged by size: 2481 2330 559 496 138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2242 2591 3020 1163 Size: 573 506 349 429 4147 Fragments arranged by size: 4147 573 506 429 349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2526 3209 4065 4150 5221 2526 Size: 683 856 85 1071 3309 Fragments arranged by size: 3309 1071 856 683 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 BsmBI CGTCTCn'nnnn_ Cuts at: 2207 2207 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2526 2651 2967 2981 3111 3209 3235 Size: 399 736 125 316 14 130 98 26 Cuts at: 3235 3323 4065 4150 5221 1391 Size: 88 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 736 399 316 130 125 98 88 85 26 14 BspEI T'CCGG_A Cuts at: 2931 5667 2931 Size: 2736 3268 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 2511 2556 2639 2865 3042 3292 3359 Size: 1328 339 45 83 226 177 250 67 Cuts at: 3359 844 Size: 3489 Fragments arranged by size: 3489 1328 339 250 226 177 83 67 45 BsrBI CCG'CTC Cuts at: 1394 1823 2848 3891 1394 Size: 429 1025 1043 3507 Fragments arranged by size: 3507 1043 1025 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2561 2884 2994 3279 4648 4835 1700 Size: 110 751 323 110 285 1369 187 2869 Fragments arranged by size: 2869 1369 751 323 285 187 110 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2419 3255 794 Size: 1625 836 3543 Fragments arranged by size: 3543 1625 836 BstBI TT'CG_AA Cuts at: 2769 4808 2769 Size: 2039 3965 BstEII G'GTnAC_C Cuts at: 1184 2731 1184 Size: 1547 4457 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2686 2011 Size: 675 5329 BstYI r'GATC_y Cuts at: 1972 2238 2265 2910 3273 3334 3701 4086 4103 Size: 266 27 645 363 61 367 385 17 Cuts at: 4103 5555 5641 5653 5999 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 645 385 367 363 346 266 86 61 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2464 2751 513 Size: 552 939 460 287 3766 Fragments arranged by size: 3766 939 552 460 287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2603 3520 4314 4334 2603 Size: 917 794 20 4273 Fragments arranged by size: 4273 917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2595 2886 3451 Size: 54 1425 50 275 18 765 291 565 Cuts at: 3451 4340 5969 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 765 565 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 3068 3120 3934 1353 Size: 1005 710 52 814 3423 Fragments arranged by size: 3423 1005 814 710 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 3236 3250 3266 4591 4965 Size: 1064 138 1047 600 14 16 1325 374 Cuts at: 4965 5111 387 Size: 146 1280 Fragments arranged by size: 1325 1280 1064 1047 600 374 146 138 16 14 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2516 2973 4067 4664 5451 5719 1239 Size: 393 884 457 1094 597 787 268 1524 Fragments arranged by size: 1524 1094 884 787 597 457 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3164 3164 Size: 6004 EcoO109I rG'GnC_Cy Cuts at: 2161 3031 3058 3118 3157 3232 2161 Size: 870 27 60 39 75 4933 Fragments arranged by size: 4933 870 75 60 39 27 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2888 2888 Size: 6004 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 3154 5151 1678 Size: 1476 1997 2531 Fragments arranged by size: 2531 1997 1476 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2681 2705 2713 Size: 32 135 32 470 32 607 24 8 Cuts at: 2713 2737 4645 4677 4719 4751 1373 Size: 24 1908 32 42 32 2626 Fragments arranged by size: 2626 1908 607 470 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 5118 5302 1305 Size: 1302 2511 184 2007 Fragments arranged by size: 2511 2007 1302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2328 3011 3962 4321 4480 518 Size: 648 843 319 683 951 359 159 2042 Fragments arranged by size: 2042 951 843 683 648 359 319 159 MspA1I CmG'CkG Cuts at: 1606 2029 2341 2482 2925 3202 3420 4097 5245 Size: 423 312 141 443 277 218 677 1148 Cuts at: 5245 5490 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 443 423 312 277 245 218 141 NaeI GCC'GGC Cuts at: 3281 3281 Size: 6004 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NgoMIV G'CCGG_C Cuts at: 3279 3279 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2038 2413 3362 4907 1132 Size: 97 809 375 949 1545 2229 Fragments arranged by size: 2229 1545 949 809 375 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 1108 Size: 1236 4768 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 3031 3058 3118 2161 Size: 870 27 60 5047 Fragments arranged by size: 5047 870 60 27 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspOMI G'GGCC_C Cuts at: 3231 3231 Size: 6004 PstI C_TGCA'G Cuts at: 830 3356 830 Size: 2526 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2029 2341 2482 2925 3202 2029 Size: 312 141 443 277 4831 Fragments arranged by size: 4831 443 312 277 141 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 2396 4252 5964 2396 Size: 1856 1712 2436 Fragments arranged by size: 2436 1856 1712 SfcI C'TryA_G Cuts at: 826 1045 3352 4487 5168 5359 826 Size: 219 2307 1135 681 191 1471 Fragments arranged by size: 2307 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 2561 1700 Size: 861 5143 SmaI CCC'GGG Cuts at: 1872 2829 3331 1872 Size: 957 502 4545 Fragments arranged by size: 4545 957 502 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2873 4108 5009 5271 820 Size: 197 926 300 630 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 630 300 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 2413 3362 1132 Size: 1281 949 3774 Fragments arranged by size: 3774 1281 949 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 3254 1517 Size: 1737 4267 StyI C'CwwG_G Cuts at: 513 1065 2004 2988 3165 3443 513 Size: 552 939 984 177 278 3074 Fragments arranged by size: 3074 984 939 552 278 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2394 4250 5962 Size: 274 80 33 51 1443 417 1856 1712 Cuts at: 5962 96 Size: 138 Fragments arranged by size: 1856 1712 1443 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 5964 5996 2204 Size: 32 3728 32 2212 Fragments arranged by size: 3728 2212 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 2827 3329 1870 Size: 957 502 4545 Fragments arranged by size: 4545 957 502 XmnI GAAnn'nnTTC Cuts at: 2444 4133 2444 Size: 1689 4315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI