################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 238 GTAGAC GT^[AC][GT]AC AlwNI 590 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 871 CCTGAGG CC^T[ACGT]AGG Aor51HI 170 AGCGCT AGC^GCT AspHI 112 GTGCTC G[AT]GC[AT]^C BalI 2 TGGCCA TGG^CCA BanII 62 GAGCCC G[GA]GC[CT]^C BbeI 137 GGCGCC GGCGC^C BbsI 310 GAAGAC GAAGAC(2/6) BciVI 86 GTATCC GTATCC(6/5) BglII 387 AGATCT A^GATCT BpuAI 310 GAAGAC GAAGAC(2/6) BsaI 181 GAGACC GGTCTC(1/5) BsiHKAI 112 GTGCTC G[AT]GC[AT]^C BspDI 789 ATCGAT AT^CGAT BspMI 581 GCAGGT ACCTGC(4/8) BsrBI 150 GAGCGG CCGCTC(-3/-3) BsrFI 47 GCCGGC [GA]^CCGG[CT] BsrI 524 ACTGG ACTGG(1/-1) BstAPI 589 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 871 CCTGAGG CC^T[ACGT]AGG Cfr10I 47 GCCGGC [GA]^CCGG[CT] ClaI 789 ATCGAT AT^CGAT CpoI 153 CGGTCCG CG^G[AT]CCG DraIII 471 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2 TGGCCA [CT]^GGCC[GA] EarI 737 GAAGAG CTCTTC(1/4) Eco47III 170 AGCGCT AGC^GCT Eco81I 871 CCTGAGG CC^T[ACGT]AGG EcoRI 484 GAATTC G^AATTC KasI 137 GGCGCC G^GCGCC Ksp632I 737 GAAGAG CTCTTC(1/4) MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspA1I 415 CAGCGG C[AC]G^C[GT]G NaeI 47 GCCGGC GCC^GGC NarI 137 GGCGCC GG^CGCC NgoMIV 47 GCCGGC G^CCGGC PstI 537 CTGCAG CTGCA^G RsrII 153 CGGTCCG CG^G[AT]CCG SfoI 137 GGCGCC GGC^GCC SspI 808 AATATT AAT^ATT StuI 716 AGGCCT AGG^CCT TaqI 790 TCGA T^CGA TthHB8I 790 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 117 CGCG CG^CG 194 CGCG AcsI 215 AAATTC [GA]^AATT[CT] 484 GAATTC AcyI 79 GGCGTC G[GA]^CG[CT]C 137 GGCGCC AfaI 207 GTAC GT^AC 349 GTAC AflIII 234 ACATGT A^C[GA][CT]GT 422 ACATGT AluI 594 AGCT AG^CT 782 AGCT ApoI 215 AAATTC [GA]^AATT[CT] 484 GAATTC AvaI 129 CCCGAG C^[CT]CG[GA]G 178 CCCGAG BamHI 628 GGATCC G^GATCC 765 GGATCC BanI 137 GGCGCC G^G[CT][GA]CC 160 GGCACC BpmI 433 CTGGAG CTGGAG(16/14) 525 CTGGAG BsaHI 79 GGCGTC G[GA]^CG[CT]C 137 GGCGCC BsoBI 129 CCCGAG C^[CT]CG[GA]G 178 CCCGAG BspLU11I 234 ACATGT A^CATGT 422 ACATGT BstUI 117 CGCG CG^CG 194 CGCG HaeII 137 GGCGCC [GA]GCGC^[CT] 170 AGCGCT HgaI 80 GCGTC GACGC(5/10) 200 GCGTC Hin1I 79 GGCGTC G[GA]^CG[CT]C 137 GGCGCC MvnI 117 CGCG CG^CG 194 CGCG NspI 234 ACATGT [GA]CATG^[CT] 422 ACATGT RsaI 207 GTAC GT^AC 349 GTAC SfcI 537 CTGCAG C^T[GA][CT]AG 834 CTACAG XcmI 66 CCAACTCTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 773 CCATAAAAGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AlwI AvaII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp509I TspRI XhoII