(Circular) (Six-base) MAPSORT of: pFN21AE6230.seq Check: 4608 from: 1 to: 5605 pFN21AE6230 5605 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2244 2948 1937 Size: 307 704 4594 Fragments arranged by size: 4594 704 307 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2331 2843 3668 5052 5320 1632 Size: 699 512 825 1384 268 1917 Fragments arranged by size: 1917 1384 825 699 512 268 AfeI AGC'GCT Cuts at: 2177 2177 Size: 5605 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2239 2427 4504 5481 1203 Size: 1036 188 2077 977 1327 Fragments arranged by size: 2077 1327 1036 977 188 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 2600 3348 4920 2600 Size: 748 1572 3285 Fragments arranged by size: 3285 1572 748 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2220 2489 2914 3140 3292 5401 1493 Size: 727 269 425 226 152 2109 1697 Fragments arranged by size: 2109 1697 727 425 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2134 2183 2930 1870 Size: 73 191 49 747 4545 Fragments arranged by size: 4545 747 191 73 49 BamHI G'GATC_C Cuts at: 2633 2770 2935 3302 2633 Size: 137 165 367 4936 Fragments arranged by size: 4936 367 165 137 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2165 2926 4381 618 Size: 325 217 627 355 23 761 1455 1842 Fragments arranged by size: 1842 1455 761 627 355 325 217 23 BanII G_rGCy'C Cuts at: 1391 2071 2924 1391 Size: 680 853 4072 Fragments arranged by size: 4072 853 680 BbeI G_GCGC'C Cuts at: 2146 2146 Size: 5605 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 928 Size: 971 423 4211 Fragments arranged by size: 4211 971 423 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2102 3497 4707 2102 Size: 1395 1210 3000 Fragments arranged by size: 3000 1395 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2392 5600 2392 Size: 3208 2397 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2168 3666 4822 1790 Size: 378 1498 1156 2573 Fragments arranged by size: 2573 1498 1156 378 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2090 2090 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 2551 4265 1239 Size: 1220 92 1714 2579 Fragments arranged by size: 2579 1714 1220 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 4268 882 Size: 617 681 2088 2219 Fragments arranged by size: 2219 2088 681 617 BsaAI yAC'GTr Cuts at: 493 1204 3396 493 Size: 711 2192 2702 Fragments arranged by size: 2702 2192 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2143 3412 3794 Size: 53 83 186 936 552 58 1269 382 Cuts at: 3794 4429 275 Size: 635 1451 Fragments arranged by size: 1451 1269 936 635 552 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5605 BseYI C'CCAG_C Cuts at: 4808 5304 4808 Size: 496 5109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5032 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 3666 3751 4822 2121 Size: 1545 85 1071 2904 Fragments arranged by size: 2904 1545 1071 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2121 2168 2924 3666 3751 4822 Size: 399 281 50 47 756 742 85 1071 Cuts at: 4822 1391 Size: 2174 Fragments arranged by size: 2174 1071 756 742 399 281 85 50 47 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2577 2960 844 Size: 1733 383 3489 Fragments arranged by size: 3489 1733 383 BsrBI CCG'CTC Cuts at: 1394 1823 2157 3492 1394 Size: 429 334 1335 3507 Fragments arranged by size: 3507 1335 429 334 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2052 4249 4436 1700 Size: 110 242 2197 187 2869 Fragments arranged by size: 2869 2197 242 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 2600 794 Size: 1806 3799 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2392 2633 2770 2935 3302 3687 3704 5156 Size: 420 241 137 165 367 385 17 1452 Cuts at: 5156 5242 5254 5600 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 420 385 367 346 241 165 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2877 2877 Size: 5605 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2215 487 Size: 683 56 989 3877 Fragments arranged by size: 3877 989 683 56 BtsI GCAGTG_nn' Cuts at: 2156 3121 3915 3935 2156 Size: 965 794 20 3826 Fragments arranged by size: 3826 965 794 20 ClaI AT'CG_AT Cuts at: 1554 2795 1554 Size: 1241 4364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2481 2481 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3052 3941 Size: 54 1425 50 275 18 177 1045 889 Cuts at: 3941 5570 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1045 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2737 3535 1353 Size: 1384 798 3423 Fragments arranged by size: 3423 1384 798 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2459 2551 2843 3668 4265 5052 Size: 393 699 128 92 292 825 597 787 Cuts at: 5052 5320 1239 Size: 268 1524 Fragments arranged by size: 1524 825 787 699 597 393 292 268 128 92 EcoRI G'AATT_C Cuts at: 2489 2914 3292 2489 Size: 425 378 4802 Fragments arranged by size: 4802 425 378 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2146 2179 4752 1678 Size: 468 33 2573 2531 Fragments arranged by size: 2573 2531 468 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2777 2809 2870 2902 4246 Size: 32 135 32 1205 32 61 32 1344 Cuts at: 4246 4278 4320 4352 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1344 1205 135 61 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KasI G'GCGC_C Cuts at: 2142 2142 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4719 4903 1305 Size: 3414 184 2007 Fragments arranged by size: 3414 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3606 Fragments arranged by size: 3606 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 3922 4081 518 Size: 648 2397 359 159 2042 Fragments arranged by size: 2397 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2422 3021 3698 4846 5091 1606 Size: 816 599 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 599 245 NaeI GCC'GGC Cuts at: 2054 2054 Size: 5605 NarI GG'CG_CC Cuts at: 2143 2143 Size: 5605 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NgoMIV G'CCGG_C Cuts at: 2052 2052 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2243 2431 2963 4508 1132 Size: 97 1014 188 532 1545 2229 Fragments arranged by size: 2229 1545 1014 532 188 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 2239 2427 4504 2239 Size: 188 2077 3340 Fragments arranged by size: 3340 2077 188 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 2801 3175 2801 Size: 374 5231 PstI C_TGCA'G Cuts at: 830 2546 2957 830 Size: 1716 411 3478 Fragments arranged by size: 3478 1716 411 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 RsrII CG'GwC_CG Cuts at: 2159 2159 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2542 2839 2953 4088 4769 4960 826 Size: 219 1497 297 114 1135 681 191 1471 Fragments arranged by size: 1497 1471 1135 681 297 219 191 114 SfoI GGC'GCC Cuts at: 2144 2144 Size: 5605 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2932 1872 Size: 1060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 2815 3529 5 Size: 47 2763 714 2081 Fragments arranged by size: 2763 2081 714 47 StuI AGG'CCT Cuts at: 1517 2723 1517 Size: 1206 4399 StyI C'CwwG_G Cuts at: 513 1065 2004 3044 513 Size: 552 939 1040 3074 Fragments arranged by size: 3074 1040 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2716 3632 3649 3802 3987 4432 325 Size: 1255 1136 916 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1136 916 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3851 5563 Size: 274 80 33 51 1443 234 1640 1712 Cuts at: 5563 96 Size: 138 Fragments arranged by size: 1712 1640 1443 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2078 2785 2078 Size: 707 4898 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2930 1870 Size: 1060 4545 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI