(Linear) (Six-base) MAPSORT of: KIEE6230.seq Check: 2498 from: 1 to: 900 KIEE6230.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AccI GT'mk_AC Cuts at: 0 239 900 Size: 239 661 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 838 900 Size: 326 512 62 Fragments arranged by size: 512 326 62 AfeI AGC'GCT Cuts at: 0 172 900 Size: 172 728 AflIII A'CryG_T Cuts at: 0 234 422 900 Size: 234 188 478 Fragments arranged by size: 478 234 188 AlwNI CAG_nnn'CTG Cuts at: 0 595 900 Size: 595 305 ApoI r'AATT_y Cuts at: 0 215 484 900 Size: 215 269 416 Fragments arranged by size: 416 269 215 AvaI C'yCGr_G Cuts at: 0 129 178 900 Size: 129 49 722 Fragments arranged by size: 722 129 49 BamHI G'GATC_C Cuts at: 0 628 765 900 Size: 628 137 135 Fragments arranged by size: 628 137 135 BanI G'GyrC_C Cuts at: 0 137 160 900 Size: 137 23 740 Fragments arranged by size: 740 137 23 BanII G_rGCy'C Cuts at: 0 66 900 Size: 66 834 BbeI G_GCGC'C Cuts at: 0 141 900 Size: 141 759 BbsI GAAGACnn'nnnn_ Cuts at: 0 317 900 Size: 317 583 BciVI GTATCCnnnnn_n' Cuts at: 0 97 900 Size: 97 803 BglII A'GATC_T Cuts at: 0 387 900 Size: 387 513 Bme1580I G_kGCm'C Cuts at: 0 163 900 Size: 163 737 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 85 900 Size: 85 815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 546 900 Size: 454 92 354 Fragments arranged by size: 454 354 92 BsaI GGTCTCn'nnnn_ Cuts at: 0 175 900 Size: 175 725 BsaHI Gr'CG_yC Cuts at: 0 80 138 900 Size: 80 58 762 Fragments arranged by size: 762 80 58 BsiHKAI G_wGCw'C Cuts at: 0 116 900 Size: 116 784 Bsp1286I G_dGCh'C Cuts at: 0 66 116 163 900 Size: 66 50 47 737 Fragments arranged by size: 737 66 50 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 572 900 Size: 572 328 BsrBI CCG'CTC Cuts at: 0 152 900 Size: 152 748 BsrFI r'CCGG_y Cuts at: 0 47 900 Size: 47 853 BstAPI GCAn_nnn'nTGC Cuts at: 0 595 900 Size: 595 305 BstYI r'GATC_y Cuts at: 0 387 628 765 900 Size: 387 241 137 135 Fragments arranged by size: 387 241 137 135 Bsu36I CC'TnA_GG Cuts at: 0 872 900 Size: 872 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 210 900 Size: 210 690 BtsI GCAGTG_nn' Cuts at: 0 151 900 Size: 151 749 ClaI AT'CG_AT Cuts at: 0 790 900 Size: 790 110 DraIII CAC_nnn'GTG Cuts at: 0 476 900 Size: 476 424 EaeI y'GGCC_r Cuts at: 0 2 900 Size: 2 898 EarI CTCTTCn'nnn_ Cuts at: 0 732 900 Size: 732 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 454 546 838 900 Size: 326 128 92 292 62 Fragments arranged by size: 326 292 128 92 62 EcoRI G'AATT_C Cuts at: 0 484 900 Size: 484 416 HaeII r_GCGC'y Cuts at: 0 141 174 900 Size: 141 33 726 Fragments arranged by size: 726 141 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 772 804 865 897 900 Size: 772 32 61 32 3 Fragments arranged by size: 772 61 32 32 3 KasI G'GCGC_C Cuts at: 0 137 900 Size: 137 763 MscI TGG'CCA Cuts at: 0 4 900 Size: 4 896 MspA1I CmG'CkG Cuts at: 0 417 900 Size: 417 483 NaeI GCC'GGC Cuts at: 0 49 900 Size: 49 851 NarI GG'CG_CC Cuts at: 0 138 900 Size: 138 762 NgoMIV G'CCGG_C Cuts at: 0 47 900 Size: 47 853 NspI r_CATG'y Cuts at: 0 238 426 900 Size: 238 188 474 Fragments arranged by size: 474 238 188 PciI A'CATG_T Cuts at: 0 234 422 900 Size: 234 188 478 Fragments arranged by size: 478 234 188 PsiI TTA'TAA Cuts at: 0 796 900 Size: 796 104 PstI C_TGCA'G Cuts at: 0 541 900 Size: 541 359 RsrII CG'GwC_CG Cuts at: 0 154 900 Size: 154 746 SfcI C'TryA_G Cuts at: 0 537 834 900 Size: 537 297 66 Fragments arranged by size: 537 297 66 SfoI GGC'GCC Cuts at: 0 139 900 Size: 139 761 SspI AAT'ATT Cuts at: 0 810 900 Size: 810 90 StuI AGG'CCT Cuts at: 0 718 900 Size: 718 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 711 900 Size: 711 189 TatI w'GTAC_w Cuts at: 0 206 900 Size: 206 694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 73 780 900 Size: 73 707 120 Fragments arranged by size: 707 120 73 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BciVI BglII Bme1580I BplI BpmI BsaI BsaHI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI Bsu36I BtgZI BtsI ClaI DraIII EaeI EarI Eco57MI EcoRI HaeII Hin4I KasI MscI MspA1I NaeI NarI NgoMIV NspI PciI PsiI PstI RsrII SfcI SfoI SspI StuI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StyI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI