################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbsI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 994 CGCG CG^CG Alw44I 484 GTGCAC G^TGCAC AlwNI 1141 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 973 CCTGAGG CC^T[ACGT]AGG ApaI 74 GGGCCC GGGCC^C ApaLI 484 GTGCAC G^TGCAC AvaI 1250 CCCGAG C^[CT]CG[GA]G BbrPI 1330 CACGTG CAC^GTG BlpI 930 GCTAAGC GC^T[ACGT]AGC Bpu1102I 930 GCTAAGC GC^T[ACGT]AGC BsaAI 1330 CACGTG [CT]AC^GT[GA] BsaBI 426 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 737 GGTCTC GGTCTC(1/5) BsmBI 304 CGTCTC CGTCTC(1/5) BsoBI 1250 CCCGAG C^[CT]CG[GA]G BspMI 218 ACCTGC ACCTGC(4/8) BsrBI 299 CCGCTC CCGCTC(-3/-3) BsrFI 1076 GCCGGT [GA]^CCGG[CT] BssSI 1315 CTCGTG CACGAG(-5/-1) BstBI 1346 TTCGAA TT^CGAA BstUI 994 CGCG CG^CG BstYI 533 AGATCC [GA]^GATC[CT] Bsu36I 973 CCTGAGG CC^T[ACGT]AGG CelII 930 GCTAAGC GC^T[ACGT]AGC Cfr10I 1076 GCCGGT [GA]^CCGG[CT] DsaI 65 CCGTGG C^C[GA][CT]GG EarI 154 CTCTTC CTCTTC(1/4) Eco81I 973 CCTGAGG CC^T[ACGT]AGG EcoRV 721 GATATC GAT^ATC HinfI 369 GAATC G^A[ACGT]TC Ksp632I 154 CTCTTC CTCTTC(1/4) MamI 426 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 533 AGATCC [GA]^GATC[CT] MvnI 994 CGCG CG^CG NspV 1346 TTCGAA TT^CGAA PmaCI 1330 CACGTG CAC^GTG PmlI 1330 CACGTG CAC^GTG PpuMI 100 AGGACCT [GA]G^G[AT]CC[CT] SacI 589 GAGCTC GAGCT^C SapI 153 GCTCTTC GCTCTTC(1/4) SfcI 829 CTACAG C^T[GA][CT]AG SfuI 1346 TTCGAA TT^CGAA SmlI 629 CTCAAG C^T[CT][GA]AG TfiI 369 GAATC G^A[AT]TC Tsp45I 864 GTCAC ^GT[GC]AC XhoII 533 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 827 GTCTAC GT^[AC][GT]AC 848 GTCTAC AfaI 822 GTAC GT^AC 1138 GTAC AflIII 383 ACATGT A^C[GA][CT]GT 492 ACATGT AlwI 534 GATCC GGATC(4/5) 702 GATCC AspHI 484 GTGCAC G[AT]GC[AT]^C 589 GAGCTC BbvCI 82 GCTGAGG CCTCAGC(-5/-2) 96 GCTGAGG BcgI 1056 CGAGGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1338 GCATCTACTTCG BseRI 46 GAGGAG GAGGAG(10/8) 1168 GAGGAG BsgI 202 GTGCAG GTGCAG(16/14) 919 GTGCAG BsiHKAI 484 GTGCAC G[AT]GC[AT]^C 589 GAGCTC BsmAI 305 GTCTC GTCTC(1/5) 738 GTCTC BspLU11I 383 ACATGT A^CATGT 492 ACATGT BsrDI 342 CATTGC GCAATG(2/0) 888 CATTGC FauI 986 CCCGC CCCGC(4/6) 1387 GCGGG MaeII 846 ACGT A^CGT 1331 ACGT MaeIII 864 GTCAC ^GT[ACGT]AC 1112 GTAAC PflMI 5 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 578 CCAAAGCCTGG RsaI 822 GTAC GT^AC 1138 GTAC SphI 944 GCATGC GCATG^C 1365 GCATGC Van91I 5 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 578 CCAAAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I XcmI