(Circular) (Six-base) MAPSORT of: pFN21AE4454.seq Check: 3985 from: 1 to: 6127 pFN21AE4454 6127 bp With 182 enzymes: SgfI * March 28, 2012 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2833 2854 3470 1937 Size: 896 21 616 4594 Fragments arranged by size: 4594 896 616 21 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3182 4190 5574 5842 1632 Size: 1550 1008 1384 268 1917 Fragments arranged by size: 1917 1550 1384 1008 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2388 2497 5026 6003 1203 Size: 1185 109 2529 977 1327 Fragments arranged by size: 2529 1327 1185 977 109 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2217 1184 Size: 1033 5094 AlwNI CAG_nnn'CTG Cuts at: 3151 3870 5442 3151 Size: 719 1572 3836 Fragments arranged by size: 3836 1572 719 ApaI G_GGCC'C Cuts at: 2083 2083 Size: 6127 ApaLI G'TGCA_C Cuts at: 2489 4184 5340 2489 Size: 1695 1156 3276 Fragments arranged by size: 3276 1695 1156 ApoI r'AATT_y Cuts at: 1493 3436 3662 3814 5923 1493 Size: 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3255 3452 1870 Size: 73 1312 197 4545 Fragments arranged by size: 4545 1312 197 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4903 618 Size: 325 217 627 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2083 2121 2598 3446 1391 Size: 692 38 477 848 4072 Fragments arranged by size: 4072 848 692 477 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5156 BbvCI CC'TCA_GC Cuts at: 2088 2102 2088 Size: 14 6113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3050 3084 3332 3366 4318 4352 1903 Size: 34 1113 34 248 34 952 34 3678 Fragments arranged by size: 3678 1113 952 248 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 2936 3544 2936 Size: 608 5519 Bme1580I G_kGCm'C Cuts at: 1790 2083 2185 2493 2888 4188 5344 1790 Size: 293 102 308 395 1300 1156 2573 Fragments arranged by size: 2573 1300 1156 395 308 293 102 BmgBI CAC'GTC Cuts at: 1318 2852 1318 Size: 1534 4593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 2172 3858 Size: 416 53 83 399 24 609 146 1686 Cuts at: 3858 4816 442 Size: 958 1753 Fragments arranged by size: 1753 1686 958 609 416 399 146 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3199 3199 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2282 2882 2975 4787 1239 Size: 971 72 600 93 1812 2579 Fragments arranged by size: 2579 1812 971 600 93 72 Bpu10I CC'TnA_GC Cuts at: 2088 2102 3213 2088 Size: 14 1111 5002 Fragments arranged by size: 5002 1111 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2619 4252 5117 5415 2619 Size: 1633 865 298 3331 Fragments arranged by size: 3331 1633 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2748 4790 882 Size: 617 1249 2042 2219 Fragments arranged by size: 2219 2042 1249 617 BsaAI yAC'GTr Cuts at: 493 1204 3337 3918 493 Size: 711 2133 581 2702 Fragments arranged by size: 2702 2133 711 581 BsaBI GATnn'nnATC Cuts at: 2435 6121 2435 Size: 3686 2441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3387 3417 1178 Size: 30 165 30 1984 30 3888 Fragments arranged by size: 3888 1984 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 3188 1186 Size: 880 1122 4125 Fragments arranged by size: 4125 1122 880 BseYI C'CCAG_C Cuts at: 2416 2482 2555 2901 2909 3280 5330 5826 2416 Size: 66 73 346 8 371 2050 496 2717 Fragments arranged by size: 2717 2050 496 371 346 73 66 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 2945 1163 Size: 573 492 717 4345 Fragments arranged by size: 4345 717 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 2598 4188 4273 5344 2493 Size: 105 1590 85 1071 3276 Fragments arranged by size: 3276 1590 1071 105 85 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 BsmBI CGTCTCn'nnnn_ Cuts at: 2315 2315 Size: 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2121 2185 2493 2598 2888 3446 Size: 399 293 38 64 308 105 290 558 Cuts at: 3446 4188 4273 5344 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 558 399 308 293 290 105 85 64 38 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 3482 844 Size: 1388 1250 3489 Fragments arranged by size: 3489 1388 1250 BsrBI CCG'CTC Cuts at: 1394 1823 2306 4014 1394 Size: 429 483 1708 3507 Fragments arranged by size: 3507 1708 483 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2346 2892 4622 4796 65 Size: 1183 1098 546 1730 174 1396 Fragments arranged by size: 1730 1396 1183 1098 546 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3081 4771 4958 1700 Size: 110 1271 1690 187 2869 Fragments arranged by size: 2869 1690 1271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 3320 4187 5199 3320 Size: 867 1012 4248 Fragments arranged by size: 4248 1012 867 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 3352 4931 3352 Size: 1579 4548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2617 2905 2909 2617 Size: 288 4 5835 Fragments arranged by size: 5835 288 4 BstYI r'GATC_y Cuts at: 1972 2538 3457 3824 4209 4226 5678 5764 5776 Size: 566 919 367 385 17 1452 86 12 Cuts at: 5776 6122 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 919 566 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2979 2979 Size: 6127 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 4570 Fragments arranged by size: 4570 939 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3040 487 Size: 683 56 1814 3574 Fragments arranged by size: 3574 1814 683 56 BtsI GCAGTG_nn' Cuts at: 3643 4437 4457 3643 Size: 794 20 5313 Fragments arranged by size: 5313 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2688 2776 Size: 54 1425 50 275 18 177 681 88 Cuts at: 2776 2971 3574 4463 6092 8 Size: 195 603 889 1629 43 Fragments arranged by size: 1629 1425 889 681 603 275 195 177 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 4057 1353 Size: 812 1892 3423 Fragments arranged by size: 3423 1892 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 2596 Size: 6127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2282 2882 2975 3182 4190 4787 Size: 393 578 72 600 93 207 1008 597 Cuts at: 4787 5574 5842 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 787 600 597 578 393 268 207 93 72 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2087 2339 2030 Size: 57 252 5818 Fragments arranged by size: 5818 252 57 EcoO109I rG'GnC_Cy Cuts at: 2080 2106 2464 3064 3148 3180 2080 Size: 26 358 600 84 32 5027 Fragments arranged by size: 5027 600 358 84 32 26 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 EcoRV GAT'ATC Cuts at: 2728 2728 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 5274 1678 Size: 3596 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2487 2519 3387 3419 4768 Size: 32 135 32 915 32 868 32 1349 Cuts at: 4768 4800 4842 4874 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1349 915 868 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2734 5241 5425 1305 Size: 1429 2507 184 2007 Fragments arranged by size: 2507 2007 1429 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2690 2778 2973 10 Size: 54 1425 343 177 681 88 195 3164 Fragments arranged by size: 3164 1425 681 343 195 177 88 54 MslI CAynn'nnrTG Cuts at: 518 1166 2496 2702 3194 3375 4085 4444 4603 Size: 648 1330 206 492 181 710 359 159 Cuts at: 4603 518 Size: 2042 Fragments arranged by size: 2042 1330 710 648 492 359 206 181 159 MspA1I CmG'CkG Cuts at: 1606 2486 2905 3160 3392 3543 4220 5368 5613 Size: 880 419 255 232 151 677 1148 245 Cuts at: 5613 1606 Size: 2120 Fragments arranged by size: 2120 1148 880 677 419 255 245 232 151 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4636 Fragments arranged by size: 4636 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2392 2501 2818 2876 2953 3374 3485 Size: 97 1163 109 317 58 77 421 111 Cuts at: 3485 5030 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1163 421 317 111 109 97 77 58 PasI CC'CwG_GG Cuts at: 1348 2572 1348 Size: 1224 4903 PciI A'CATG_T Cuts at: 2388 2497 5026 2388 Size: 109 2529 3489 Fragments arranged by size: 3489 2529 109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2016 2589 1108 Size: 908 573 4646 Fragments arranged by size: 4646 908 573 PfoI T'CCnGG_A Cuts at: 2259 2474 2802 3405 2259 Size: 215 328 603 4981 Fragments arranged by size: 4981 603 328 215 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PmlI CAC'GTG Cuts at: 3337 3337 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2106 2106 Size: 6127 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 2079 2079 Size: 6127 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2486 2905 3160 2486 Size: 419 255 5453 Fragments arranged by size: 5453 419 255 SacI G_AGCT'C Cuts at: 2598 2598 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SapI GCTCTTCn'nnn_ Cuts at: 2165 2165 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2834 3475 4610 5291 5482 826 Size: 219 1789 641 1135 681 191 1471 Fragments arranged by size: 1789 1471 1135 681 641 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2634 4231 5132 5394 820 Size: 197 926 691 1597 901 262 1553 Fragments arranged by size: 1597 1553 926 901 691 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 2953 3374 3485 1132 Size: 1821 421 111 3774 Fragments arranged by size: 3774 1821 421 111 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2004 3566 513 Size: 552 939 1562 3074 Fragments arranged by size: 3074 1562 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2623 2772 2915 3023 4154 4171 4324 Size: 1255 1043 149 143 108 1131 17 153 Cuts at: 4324 4509 4954 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1131 1043 445 185 153 149 143 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2826 4373 6085 Size: 274 80 33 51 1443 849 1547 1712 Cuts at: 6085 96 Size: 138 Fragments arranged by size: 1712 1547 1443 849 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2563 3107 2185 Size: 378 544 5205 Fragments arranged by size: 5205 544 378 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI