(Linear) (Six-base) MAPSORT of: KIEE4454.seq Check: 7359 from: 1 to: 1422 KIEE4454.orf With 182 enzymes: SgfI * March 22, 2012 18:04 .. AccI GT'mk_AC Cuts at: 0 828 849 1422 Size: 828 21 573 Fragments arranged by size: 828 573 21 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1177 1422 Size: 1177 245 AflIII A'CryG_T Cuts at: 0 383 492 1422 Size: 383 109 930 Fragments arranged by size: 930 383 109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 212 1422 Size: 212 1210 AlwNI CAG_nnn'CTG Cuts at: 0 1146 1422 Size: 1146 276 ApaI G_GGCC'C Cuts at: 0 78 1422 Size: 78 1344 ApaLI G'TGCA_C Cuts at: 0 484 1422 Size: 484 938 AvaI C'yCGr_G Cuts at: 0 1250 1422 Size: 1250 172 BanII G_rGCy'C Cuts at: 0 78 116 593 1422 Size: 78 38 477 829 Fragments arranged by size: 829 477 78 38 BbvCI CC'TCA_GC Cuts at: 0 83 97 1422 Size: 83 14 1325 Fragments arranged by size: 1325 83 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1045 1079 1327 1361 1422 Size: 1045 34 248 34 61 Fragments arranged by size: 1045 248 61 34 34 BlpI GC'TnA_GC Cuts at: 0 931 1422 Size: 931 491 Bme1580I G_kGCm'C Cuts at: 0 78 180 488 883 1422 Size: 78 102 308 395 539 Fragments arranged by size: 539 395 308 102 78 BmgBI CAC'GTC Cuts at: 0 847 1422 Size: 847 575 BmrI ACTGGGnnnn_n' Cuts at: 0 21 167 1422 Size: 21 146 1255 Fragments arranged by size: 1255 146 21 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1194 1422 Size: 1194 228 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 277 877 970 1422 Size: 205 72 600 93 452 Fragments arranged by size: 600 452 205 93 72 Bpu10I CC'TnA_GC Cuts at: 0 83 97 1208 1422 Size: 83 14 1111 214 Fragments arranged by size: 1111 214 83 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 614 1422 Size: 614 808 BsaI GGTCTCn'nnnn_ Cuts at: 0 743 1422 Size: 743 679 BsaAI yAC'GTr Cuts at: 0 1332 1422 Size: 1332 90 BsaBI GATnn'nnATC Cuts at: 0 430 1422 Size: 430 992 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1382 1412 1422 Size: 1382 30 10 Fragments arranged by size: 1382 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 1183 1422 Size: 61 1122 239 Fragments arranged by size: 1122 239 61 BseYI C'CCAG_C Cuts at: 0 411 477 550 896 904 1275 1422 Size: 411 66 73 346 8 371 147 Fragments arranged by size: 411 371 346 147 73 66 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 940 1422 Size: 223 717 482 Fragments arranged by size: 717 482 223 BsiHKAI G_wGCw'C Cuts at: 0 488 593 1422 Size: 488 105 829 Fragments arranged by size: 829 488 105 BsmBI CGTCTCn'nnnn_ Cuts at: 0 310 1422 Size: 310 1112 Bsp1286I G_dGCh'C Cuts at: 0 78 116 180 488 593 883 1422 Size: 78 38 64 308 105 290 539 Fragments arranged by size: 539 308 290 105 78 64 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 227 1422 Size: 227 1195 BsrBI CCG'CTC Cuts at: 0 301 1422 Size: 301 1121 BsrDI GCAATG_nn' Cuts at: 0 341 887 1422 Size: 341 546 535 Fragments arranged by size: 546 535 341 BsrFI r'CCGG_y Cuts at: 0 1076 1422 Size: 1076 346 BssSI C'ACGA_G Cuts at: 0 1315 1422 Size: 1315 107 BstBI TT'CG_AA Cuts at: 0 1347 1422 Size: 1347 75 BstXI CCAn_nnnn'nTGG Cuts at: 0 612 900 904 1422 Size: 612 288 4 518 Fragments arranged by size: 612 518 288 4 BstYI r'GATC_y Cuts at: 0 533 1422 Size: 533 889 Bsu36I CC'TnA_GG Cuts at: 0 974 1422 Size: 974 448 BtgI C'CryG_G Cuts at: 0 65 1422 Size: 65 1357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1035 1422 Size: 1035 387 EaeI y'GGCC_r Cuts at: 0 2 683 771 966 1422 Size: 2 681 88 195 456 Fragments arranged by size: 681 456 195 88 2 EarI CTCTTCn'nnn_ Cuts at: 0 160 1422 Size: 160 1262 EcoICRI GAG'CTC Cuts at: 0 591 1422 Size: 591 831 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 277 877 970 1177 1422 Size: 205 72 600 93 207 245 Fragments arranged by size: 600 245 207 205 93 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 82 334 1422 Size: 25 57 252 1088 Fragments arranged by size: 1088 252 57 25 EcoO109I rG'GnC_Cy Cuts at: 0 75 101 459 1059 1143 1175 1422 Size: 75 26 358 600 84 32 247 Fragments arranged by size: 600 358 247 84 75 32 26 EcoRV GAT'ATC Cuts at: 0 723 1422 Size: 723 699 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 482 514 1382 1414 1422 Size: 482 32 868 32 8 Fragments arranged by size: 868 482 32 32 8 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 729 1422 Size: 729 693 MscI TGG'CCA Cuts at: 0 4 685 773 968 1422 Size: 4 681 88 195 454 Fragments arranged by size: 681 454 195 88 4 MslI CAynn'nnrTG Cuts at: 0 491 697 1189 1370 1422 Size: 491 206 492 181 52 Fragments arranged by size: 492 491 206 181 52 MspA1I CmG'CkG Cuts at: 0 481 900 1155 1387 1422 Size: 481 419 255 232 35 Fragments arranged by size: 481 419 255 232 35 NspI r_CATG'y Cuts at: 0 387 496 813 871 948 1369 1422 Size: 387 109 317 58 77 421 53 Fragments arranged by size: 421 387 317 109 77 58 53 PasI CC'CwG_GG Cuts at: 0 567 1422 Size: 567 855 PciI A'CATG_T Cuts at: 0 383 492 1422 Size: 383 109 930 Fragments arranged by size: 930 383 109 PflMI CCAn_nnn'nTGG Cuts at: 0 11 584 1422 Size: 11 573 838 Fragments arranged by size: 838 573 11 PfoI T'CCnGG_A Cuts at: 0 254 469 797 1400 1422 Size: 254 215 328 603 22 Fragments arranged by size: 603 328 254 215 22 PmlI CAC'GTG Cuts at: 0 1332 1422 Size: 1332 90 PpuMI rG'GwC_Cy Cuts at: 0 101 1422 Size: 101 1321 PspOMI G'GGCC_C Cuts at: 0 74 1422 Size: 74 1348 PvuII CAG'CTG Cuts at: 0 481 900 1155 1422 Size: 481 419 255 267 Fragments arranged by size: 481 419 267 255 SacI G_AGCT'C Cuts at: 0 593 1422 Size: 593 829 SapI GCTCTTCn'nnn_ Cuts at: 0 160 1422 Size: 160 1262 SfcI C'TryA_G Cuts at: 0 829 1422 Size: 829 593 SmlI C'TyrA_G Cuts at: 0 629 1422 Size: 629 793 SphI G_CATG'C Cuts at: 0 948 1369 1422 Size: 948 421 53 Fragments arranged by size: 948 421 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 618 767 910 1018 1422 Size: 618 149 143 108 404 Fragments arranged by size: 618 404 149 143 108 TatI w'GTAC_w Cuts at: 0 821 1422 Size: 821 601 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 180 558 1102 1422 Size: 180 378 544 320 Fragments arranged by size: 544 378 320 180 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI BanII BbvCI BcgI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I MmeI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PvuII SacI SapI SfcI SmlI SphI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI BfrBI BglI BglII BmtI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BstAPI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI