################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BamHI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MspA1I MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 90 CGCG CG^CG AcsI 718 GAATTC [GA]^AATT[CT] AcyI 3 GGCGCC G[GA]^CG[CT]C AlwI 169 GATCC GGATC(4/5) AlwNI 58 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 718 GAATTC [GA]^AATT[CT] Asp700I 15 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 276 GGTCC G^G[AT]CC AviII 189 TGCGCA TGC^GCA BalI 662 TGGCCA TGG^CCA BanII 70 GAGCTC G[GA]GC[CT]^C BbeI 3 GGCGCC GGCGC^C BbrPI 267 CACGTG CAC^GTG BbvI 187 GCTGC GCAGC(8/12) BclI 248 TGATCA T^GATCA BcnI 155 CCCGG CC^[GC]GG BsaAI 267 CACGTG [CT]AC^GT[GA] BsaHI 3 GGCGCC G[GA]^CG[CT]C BsiEI 104 CGGCCG CG[GA][CT]^CG BsmAI 589 GTCTC GTCTC(1/5) BsmBI 588 CGTCTC CGTCTC(1/5) BsmFI 360 GGGAC GGGAC(10/14) BspMI 649 ACCTGC ACCTGC(4/8) BsrFI 102 GCCGGC [GA]^CCGG[CT] BssSI 556 CACGAG CACGAG(-5/-1) BstUI 90 CGCG CG^CG BstXI 266 CCACGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 168 AGATCC [GA]^GATC[CT] Cfr10I 102 GCCGGC [GA]^CCGG[CT] EagI 104 CGGCCG C^GGCCG EclXI 104 CGGCCG C^GGCCG Eco52I 104 CGGCCG C^GGCCG EcoRI 718 GAATTC G^AATTC EcoT14I 95 CCAAGG C^C[AT][AT]GG FbaI 248 TGATCA T^GATCA FspI 189 TGCGCA TGC^GCA HaeII 3 GGCGCC [GA]GCGC^[CT] Hin1I 3 GGCGCC G[GA]^CG[CT]C KasI 3 GGCGCC G^GCGCC MflI 168 AGATCC [GA]^GATC[CT] MluNI 662 TGGCCA TGG^CCA MscI 662 TGGCCA TGG^CCA MvnI 90 CGCG CG^CG NaeI 102 GCCGGC GCC^GGC NarI 3 GGCGCC GG^CGCC NciI 155 CCCGG CC^[GC]GG NgoMIV 102 GCCGGC G^CCGGC NspI 300 GCATGT [GA]CATG^[CT] PflMI 345 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 267 CACGTG CAC^GTG PmlI 267 CACGTG CAC^GTG PshAI 441 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 70 GAGCTC GAGCT^C SfoI 3 GGCGCC GGC^GCC SmlI 709 CTCAAG C^T[CT][GA]AG StyI 95 CCAAGG C^C[AT][AT]GG TseI 187 GCTGC G^C[AT]GC Tsp509I 719 AATT ^AATT TspRI 417 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 345 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 173 CCAAGATTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 168 AGATCC [GA]^GATC[CT] XmnI 15 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 515 GTAC GT^AC 553 GTAC BanI 3 GGCGCC G^G[CT][GA]CC 99 GGTGCC BfaI 166 CTAG C^TAG 394 CTAG BpmI 9 CTCCAG CTGGAG(16/14) 471 CTGGAG BsiYI 345 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 377 CCTGCATTGGG BslI 345 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 377 CCTGCATTGGG BstF5I 571 GGATG GGATG(2/0) 681 GGATG CfoI 4 GCGC GCG^C 190 GCGC Fnu4HI 91 GCGGC GC^[ACGT]GC 187 GCTGC FokI 571 GGATG GGATG(9/13) 681 GGATG HapII 103 CCGG C^CGG 156 CCGG HhaI 4 GCGC GCG^C 190 GCGC HinP1I 4 GCGC G^CGC 190 GCGC HpaII 103 CCGG C^CGG 156 CCGG ItaI 91 GCGGC GC^[ACGT]GC 187 GCTGC MaeI 166 CTAG C^TAG 394 CTAG MaeIII 208 GTGAC ^GT[ACGT]AC 637 GTGAC MboII 39 GAAGA GAAGA(8/7) 20 TCTTC MslI 111 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 267 CACGTGGGTG MspI 103 CCGG C^CGG 156 CCGG MwoI 181 GCTGTGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 324 GCTGATTGGGC RsaI 515 GTAC GT^AC 553 GTAC SfaNI 595 GATGC GCATC(5/9) 413 GCATC TaqI 146 TCGA T^CGA 434 TCGA Tsp45I 208 GTGAC ^GT[GC]AC 637 GTGAC TthHB8I 146 TCGA T^CGA 434 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI AspHI BmyI BsaJI BsiHKAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII HaeIII HinfI MaeII MboI MnlI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI