################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccIII AclI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 252 GAATTT [GA]^AATT[CT] AcyI 61 GGCGTC G[GA]^CG[CT]C AflIII 1103 ACATGT A^C[GA][CT]GT AgeI 1647 ACCGGT A^CCGGT AlwNI 1195 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 252 GAATTT [GA]^AATT[CT] BbsI 19 GTCTTC GAAGAC(2/6) BciVI 188 GGATAC GTATCC(6/5) BfaI 865 CTAG C^TAG BglII 491 AGATCT A^GATCT BpuAI 19 GTCTTC GAAGAC(2/6) BsaAI 721 TACGTG [CT]AC^GT[GA] BsaHI 61 GGCGTC G[GA]^CG[CT]C BsgI 1351 GTGCAG GTGCAG(16/14) BspLU11I 1103 ACATGT A^CATGT BspMI 850 GCAGGT ACCTGC(4/8) BsrFI 1647 ACCGGT [GA]^CCGG[CT] BssHII 9 GCGCGC G^CGCGC BstAPI 212 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1647 ACCGGT [GA]^CCGG[CT] EcoNI 603 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1255 ATGCAT ATGCA^T FauI 1273 GCGGG CCCGC(4/6) Hin1I 61 GGCGTC G[GA]^CG[CT]C HindIII 1434 AAGCTT A^AGCTT MaeI 865 CTAG C^TAG MaeII 722 ACGT A^CGT MseI 1616 TTAA T^TAA NsiI 1255 ATGCAT ATGCA^T PinAI 1647 ACCGGT A^CCGGT PstI 210 CTGCAG CTGCA^G PvuII 412 CAGCTG CAG^CTG SapI 1300 GAAGAGC GCTCTTC(1/4) SfcI 210 CTGCAG C^T[GA][CT]AG SgrAI 1646 CACCGGTG C[GA]^CCGG[CT]G SmlI 980 CTCAAG C^T[CT][GA]AG SphI 1013 GCATGC GCATG^C SspI 920 AATATT AAT^ATT TaqI 23 TCGA T^CGA Tru9I 1616 TTAA T^TAA Tsp509I 253 AATT ^AATT TthHB8I 23 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 824 GGTACC G^GTACC 1394 GGTACC AccII 10 CGCG CG^CG 12 CGCG Asp700I 342 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1482 GAAGGACTTC Asp718I 824 GGTACC G^GTACC 1394 GGTACC AspHI 219 GTGCTC G[AT]GC[AT]^C 1492 GTGCTC AvaI 802 CCCGAG C^[CT]CG[GA]G 1045 CCCGAG BalI 996 TGGCCA TGG^CCA 1064 TGGCCA BanII 765 GGGCTC G[GA]GC[CT]^C 1303 GAGCCC BbvCI 1311 CCTCAGC CCTCAGC(-5/-2) 1177 GCTGAGG BpmI 316 CTCCAG CTGGAG(16/14) 637 CTGGAG BsaI 226 GAGACC GGTCTC(1/5) 1653 GGTCTC BsaWI 649 ACCGGA [AT]^CCGG[AT] 1647 ACCGGT BsiHKAI 219 GTGCTC G[AT]GC[AT]^C 1492 GTGCTC BsmAI 226 GAGAC GTCTC(1/5) 1654 GTCTC BsoBI 802 CCCGAG C^[CT]CG[GA]G 1045 CCCGAG BstUI 10 CGCG CG^CG 12 CGCG DsaI 714 CCATGG C^C[GA][CT]GG 792 CCATGG EagI 14 CGGCCG C^GGCCG 69 CGGCCG EclXI 14 CGGCCG C^GGCCG 69 CGGCCG Eco52I 14 CGGCCG C^GGCCG 69 CGGCCG EcoT14I 714 CCATGG C^C[AT][AT]GG 792 CCATGG HaeII 42 GGCGCT [GA]GCGC^[CT] 90 GGCGCT HgaI 3 GACGC GACGC(5/10) 62 GCGTC KpnI 824 GGTACC GGTAC^C 1394 GGTACC MluNI 996 TGGCCA TGG^CCA 1064 TGGCCA MscI 996 TGGCCA TGG^CCA 1064 TGGCCA MslI 927 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1014 CATGCTGGTG MspA1I 50 CAGCGG C[AC]G^C[GT]G 412 CAGCTG MvnI 10 CGCG CG^CG 12 CGCG NcoI 714 CCATGG C^CATGG 792 CCATGG NspI 1013 GCATGC [GA]CATG^[CT] 1103 ACATGT PflMI 368 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1512 CCAGCACATGG PpuMI 860 GGGTCCT [GA]G^G[AT]CC[CT] 1380 AGGTCCT StyI 714 CCATGG C^C[AT][AT]GG 792 CCATGG Van91I 368 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1512 CCAGCACATGG XcmI 368 CCAACACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 999 CCATGACTGGGGTGG XmnI 342 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1482 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanI BbvI BcnI BmyI BsaJI BseRI BsiEI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoO65I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I TspRI XhoII