(Circular) (Six-base) MAPSORT of: pFN21AB5020.seq Check: 3853 from: 1 to: 6370 pFN21AB5020 6370 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 2829 3399 3691 2829 Size: 570 292 5508 Fragments arranged by size: 5508 570 292 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 2543 2594 3214 4433 5817 6085 1632 Size: 821 90 51 620 1219 1384 268 1917 Fragments arranged by size: 1917 1384 1219 821 620 268 90 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6173 AflIII A'CryG_T Cuts at: 1203 3108 5269 6246 1203 Size: 1905 2161 977 1327 Fragments arranged by size: 2161 1905 1327 977 AgeI A'CCGG_T Cuts at: 3652 5201 3652 Size: 1549 4821 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6370 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3332 3364 3332 Size: 32 6338 AlwNI CAG_nnn'CTG Cuts at: 3205 4113 5685 3205 Size: 908 1572 3890 Fragments arranged by size: 3890 1572 908 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 2257 3679 3905 4057 6166 1493 Size: 764 1422 226 152 2109 1697 Fragments arranged by size: 2109 1697 1422 764 226 152 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 2807 3050 3695 1870 Size: 73 864 243 645 4545 Fragments arranged by size: 4545 864 645 243 73 BamHI G'GATC_C Cuts at: 3700 4067 3700 Size: 367 6003 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2829 3399 3691 5146 Size: 325 217 627 360 682 570 292 1455 Cuts at: 5146 618 Size: 1842 Fragments arranged by size: 1842 1455 682 627 570 360 325 292 217 BanII G_rGCy'C Cuts at: 1391 2774 3312 3689 1391 Size: 1383 538 377 4072 Fragments arranged by size: 4072 1383 538 377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 928 Size: 971 118 5281 Fragments arranged by size: 5281 971 118 BbvCI CC'TCA_GC Cuts at: 3183 3317 3183 Size: 134 6236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2187 4262 5472 2187 Size: 2075 1210 3085 Fragments arranged by size: 3085 2075 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BfrBI ATG'CAT Cuts at: 3262 3262 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4981 136 Size: 107 122 71 631 605 3309 1525 Fragments arranged by size: 3309 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2496 6365 2496 Size: 3869 2501 BlpI GC'TnA_GC Cuts at: 3787 3787 Size: 6370 Bme1580I G_kGCm'C Cuts at: 1790 3576 4431 5587 1790 Size: 1786 855 1156 2573 Fragments arranged by size: 2573 1786 1156 855 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 2529 2984 Size: 416 53 83 399 24 765 347 455 Cuts at: 2984 3019 3134 4101 5059 442 Size: 35 115 967 958 1753 Fragments arranged by size: 1753 967 958 765 455 416 399 347 115 83 53 35 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2663 5030 1239 Size: 1067 357 2367 2579 Fragments arranged by size: 2579 2367 1067 357 Bpu10I CC'TnA_GC Cuts at: 2339 3183 3317 2339 Size: 844 134 5392 Fragments arranged by size: 5392 844 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2970 4495 5360 5658 2970 Size: 1525 865 298 3682 Fragments arranged by size: 3682 1525 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 3664 5033 882 Size: 617 726 1439 1369 2219 Fragments arranged by size: 2219 1439 1369 726 617 BsaAI yAC'GTr Cuts at: 493 1204 2728 4161 493 Size: 711 1524 1433 2702 Fragments arranged by size: 2702 1524 1433 711 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 4177 4559 5194 Size: 53 83 186 936 534 2110 382 635 Cuts at: 5194 275 Size: 1451 Fragments arranged by size: 2110 1451 936 635 534 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2654 3652 4803 5201 5475 5622 6033 2654 Size: 998 1151 398 274 147 411 2991 Fragments arranged by size: 2991 1151 998 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6145 Fragments arranged by size: 6145 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2898 3350 1186 Size: 913 799 452 4206 Fragments arranged by size: 4206 913 799 452 BseYI C'CCAG_C Cuts at: 2415 2767 3311 3516 5573 6069 2415 Size: 352 544 205 2057 496 2716 Fragments arranged by size: 2716 2057 544 496 352 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3377 1163 Size: 573 1641 4156 Fragments arranged by size: 4156 1641 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2077 2392 3820 4581 5609 Size: 275 186 21 55 315 1428 761 1028 Cuts at: 5609 1540 Size: 2301 Fragments arranged by size: 2301 1428 1028 761 315 275 186 55 21 BsiHKAI G_wGCw'C Cuts at: 2228 3501 4431 4516 5587 2228 Size: 1273 930 85 1071 3011 Fragments arranged by size: 3011 1273 1071 930 85 BsmI GAATG_Cn' Cuts at: 3881 3974 3881 Size: 93 6277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2774 3312 3501 3576 3689 4431 Size: 399 438 546 538 189 75 113 742 Cuts at: 4431 4516 5587 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 538 438 399 189 113 85 75 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2846 3725 844 Size: 2002 879 3489 Fragments arranged by size: 3489 2002 879 BsrBI CCG'CTC Cuts at: 1394 1823 4257 1394 Size: 429 2434 3507 Fragments arranged by size: 3507 2434 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4865 5039 65 Size: 1183 3617 174 1396 Fragments arranged by size: 3617 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3652 5014 5201 1700 Size: 110 1842 1362 187 2869 Fragments arranged by size: 2869 1842 1362 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 2014 1925 Size: 89 6281 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 2223 794 Size: 1429 4941 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 2599 2858 2956 1184 Size: 1415 259 98 4598 Fragments arranged by size: 4598 1415 259 98 BstYI r'GATC_y Cuts at: 1972 2496 2835 3453 3612 3700 4067 4452 4469 Size: 524 339 618 159 88 367 385 17 Cuts at: 4469 5921 6007 6019 6365 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 618 524 385 367 346 339 159 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2719 2797 513 Size: 552 1654 78 4086 Fragments arranged by size: 4086 1654 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3255 487 Size: 683 56 2029 3602 Fragments arranged by size: 3602 2029 683 56 BtsI GCAGTG_nn' Cuts at: 2997 3886 4680 4700 2997 Size: 889 794 20 4667 Fragments arranged by size: 4667 889 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5377 809 Size: 507 4061 1802 Fragments arranged by size: 4061 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2074 3001 Size: 54 1425 50 275 18 189 55 927 Cuts at: 3001 3069 3817 4706 6335 8 Size: 68 748 889 1629 43 Fragments arranged by size: 1629 1425 927 889 748 275 189 68 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 2074 3817 1537 Size: 275 207 55 1743 4090 Fragments arranged by size: 4090 1743 275 207 55 EarI CTCTTCn'nnn_ Cuts at: 1353 3047 3233 3300 4300 1353 Size: 1694 186 67 1000 3423 Fragments arranged by size: 3423 1694 1000 186 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3497 4957 5331 5477 387 Size: 1064 138 1908 1460 374 146 1280 Fragments arranged by size: 1908 1460 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2453 2543 2594 2663 3214 4433 Size: 393 674 147 90 51 69 551 1219 Cuts at: 4433 5030 5817 6085 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1219 787 674 597 551 393 268 147 90 69 51 EcoNI CCTnn'n_nnAGG Cuts at: 2612 2612 Size: 6370 EcoO109I rG'GnC_Cy Cuts at: 2089 2866 3386 2089 Size: 777 520 5073 Fragments arranged by size: 5073 777 520 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6370 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 2051 2099 5517 1678 Size: 373 48 3418 2531 Fragments arranged by size: 3418 2531 373 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2827 2859 3438 3470 5011 Size: 32 135 32 1255 32 579 32 1541 Cuts at: 5011 5043 5085 5117 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1541 1255 579 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3714 3960 669 Size: 988 281 1776 246 3079 Fragments arranged by size: 3079 1776 988 281 246 HindIII A'AGCT_T Cuts at: 748 3439 748 Size: 2691 3679 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 2833 3403 3695 2833 Size: 570 292 5508 Fragments arranged by size: 5508 570 292 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3191 5484 5668 1305 Size: 1886 2293 184 2007 Fragments arranged by size: 2293 2007 1886 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3003 3071 10 Size: 54 1425 343 1171 68 3309 Fragments arranged by size: 3309 1425 1171 343 68 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 3023 4328 4687 4846 518 Size: 648 1770 87 1305 359 159 2042 Fragments arranged by size: 2042 1770 1305 648 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2057 2419 3786 4463 5611 5856 1606 Size: 451 362 1367 677 1148 245 2120 Fragments arranged by size: 2120 1367 1148 677 451 362 245 NcoI C'CATG_G Cuts at: 513 1065 2719 2797 513 Size: 552 1654 78 4086 Fragments arranged by size: 4086 1654 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NsiI A_TGCA'T Cuts at: 3264 3264 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 3022 3112 3728 5273 1132 Size: 97 1793 90 616 1545 2229 Fragments arranged by size: 2229 1793 1545 616 97 90 PasI CC'CwG_GG Cuts at: 1348 2742 1348 Size: 1394 4976 PciI A'CATG_T Cuts at: 3108 5269 3108 Size: 2161 4209 PflMI CCAn_nnn'nTGG Cuts at: 1108 2379 3523 1108 Size: 1271 1144 3955 Fragments arranged by size: 3955 1271 1144 PfoI T'CCnGG_A Cuts at: 2613 2964 2613 Size: 351 6019 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2866 3386 2866 Size: 520 5850 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PstI C_TGCA'G Cuts at: 830 2219 3722 830 Size: 1389 1503 3478 Fragments arranged by size: 3478 1503 1389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2419 2419 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SapI GCTCTTCn'nnn_ Cuts at: 3300 3300 Size: 6370 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 2215 3718 4853 5534 5725 826 Size: 219 1170 1503 1135 681 191 1471 Fragments arranged by size: 1503 1471 1170 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 3652 1700 Size: 1952 4418 SmaI CCC'GGG Cuts at: 1872 3697 1872 Size: 1825 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2985 4474 5375 5637 820 Size: 197 926 1042 1489 901 262 1553 Fragments arranged by size: 1553 1489 1042 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3022 3728 1132 Size: 1890 706 3774 Fragments arranged by size: 3774 1890 706 SspI AAT'ATT Cuts at: 5 52 2927 4294 5 Size: 47 2875 1367 2081 Fragments arranged by size: 2875 2081 1367 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6370 StyI C'CwwG_G Cuts at: 513 1065 2719 2797 3809 513 Size: 552 1654 78 1012 3074 Fragments arranged by size: 3074 1654 1012 552 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4397 4414 4567 4752 5197 325 Size: 1255 2817 17 153 185 445 1498 Fragments arranged by size: 2817 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4616 6328 96 Size: 274 80 33 51 1443 2639 1712 138 Fragments arranged by size: 2639 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6330 6362 6330 Size: 32 6338 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 3011 2380 Size: 631 5739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 3695 1870 Size: 1825 4545 XmnI GAAnn'nnTTC Cuts at: 2351 3491 4499 2351 Size: 1140 1008 4222 Fragments arranged by size: 4222 1140 1008 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI