(Linear) (Six-base) MAPSORT of: KIBB5020.seq Check: 7232 from: 1 to: 1665 KIBB5020.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. Acc65I G'GTAC_C Cuts at: 0 824 1394 1665 Size: 824 570 271 Fragments arranged by size: 824 570 271 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 538 589 1209 1665 Size: 448 90 51 620 456 Fragments arranged by size: 620 456 448 90 51 AflIII A'CryG_T Cuts at: 0 1103 1665 Size: 1103 562 AgeI A'CCGG_T Cuts at: 0 1647 1665 Size: 1647 18 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1327 1359 1665 Size: 1327 32 306 Fragments arranged by size: 1327 306 32 AlwNI CAG_nnn'CTG Cuts at: 0 1200 1665 Size: 1200 465 ApoI r'AATT_y Cuts at: 0 252 1665 Size: 252 1413 AvaI C'yCGr_G Cuts at: 0 802 1045 1665 Size: 802 243 620 Fragments arranged by size: 802 620 243 BanI G'GyrC_C Cuts at: 0 142 824 1394 1665 Size: 142 682 570 271 Fragments arranged by size: 682 570 271 142 BanII G_rGCy'C Cuts at: 0 769 1307 1665 Size: 769 538 358 Fragments arranged by size: 769 538 358 BbsI GAAGACnn'nnnn_ Cuts at: 0 12 1665 Size: 12 1653 BbvCI CC'TCA_GC Cuts at: 0 1178 1312 1665 Size: 1178 134 353 Fragments arranged by size: 1178 353 134 BciVI GTATCCnnnnn_n' Cuts at: 0 182 1665 Size: 182 1483 BfrBI ATG'CAT Cuts at: 0 1257 1665 Size: 1257 408 BglII A'GATC_T Cuts at: 0 491 1665 Size: 491 1174 Bme1580I G_kGCm'C Cuts at: 0 1571 1665 Size: 1571 94 BmrI ACTGGGnnnn_n' Cuts at: 0 177 524 979 1014 1129 1665 Size: 177 347 455 35 115 536 Fragments arranged by size: 536 455 347 177 115 35 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 783 1665 Size: 783 882 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 658 1665 Size: 301 357 1007 Fragments arranged by size: 1007 357 301 Bpu10I CC'TnA_GC Cuts at: 0 334 1178 1312 1665 Size: 334 844 134 353 Fragments arranged by size: 844 353 334 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 965 1665 Size: 965 700 BsaI GGTCTCn'nnnn_ Cuts at: 0 220 1659 1665 Size: 220 1439 6 Fragments arranged by size: 1439 220 6 BsaAI yAC'GTr Cuts at: 0 723 1665 Size: 723 942 BsaHI Gr'CG_yC Cuts at: 0 62 1665 Size: 62 1603 BsaWI w'CCGG_w Cuts at: 0 649 1647 1665 Size: 649 998 18 Fragments arranged by size: 998 649 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 893 1345 1665 Size: 94 799 452 320 Fragments arranged by size: 799 452 320 94 BseYI C'CCAG_C Cuts at: 0 410 762 1306 1511 1665 Size: 410 352 544 205 154 Fragments arranged by size: 544 410 352 205 154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1372 1665 Size: 1372 293 BsiEI CG_ry'CG Cuts at: 0 17 72 387 1665 Size: 17 55 315 1278 Fragments arranged by size: 1278 315 55 17 BsiHKAI G_wGCw'C Cuts at: 0 223 1496 1665 Size: 223 1273 169 Fragments arranged by size: 1273 223 169 Bsp1286I G_dGCh'C Cuts at: 0 223 769 1307 1496 1571 1665 Size: 223 546 538 189 75 94 Fragments arranged by size: 546 538 223 189 94 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 841 1665 Size: 841 824 BsrFI r'CCGG_y Cuts at: 0 1647 1665 Size: 1647 18 BssHII G'CGCG_C Cuts at: 0 9 1665 Size: 9 1656 BstAPI GCAn_nnn'nTGC Cuts at: 0 218 1665 Size: 218 1447 BstEII G'GTnAC_C Cuts at: 0 594 853 951 1665 Size: 594 259 98 714 Fragments arranged by size: 714 594 259 98 BstYI r'GATC_y Cuts at: 0 491 830 1448 1607 1665 Size: 491 339 618 159 58 Fragments arranged by size: 618 491 339 159 58 BtgI C'CryG_G Cuts at: 0 714 792 1665 Size: 714 78 873 Fragments arranged by size: 873 714 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1250 1665 Size: 1250 415 BtsI GCAGTG_nn' Cuts at: 0 992 1665 Size: 992 673 EaeI y'GGCC_r Cuts at: 0 14 69 996 1064 1665 Size: 14 55 927 68 601 Fragments arranged by size: 927 601 68 55 14 EagI C'GGCC_G Cuts at: 0 14 69 1665 Size: 14 55 1596 Fragments arranged by size: 1596 55 14 EarI CTCTTCn'nnn_ Cuts at: 0 1042 1228 1295 1665 Size: 1042 186 67 370 Fragments arranged by size: 1042 370 186 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1492 1665 Size: 1492 173 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 448 538 589 658 1209 1665 Size: 301 147 90 51 69 551 456 Fragments arranged by size: 551 456 301 147 90 69 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 607 1665 Size: 607 1058 EcoO109I rG'GnC_Cy Cuts at: 0 84 861 1381 1665 Size: 84 777 520 284 Fragments arranged by size: 777 520 284 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 453 1665 Size: 453 1212 HaeII r_GCGC'y Cuts at: 0 46 94 1665 Size: 46 48 1571 Fragments arranged by size: 1571 48 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 822 854 1433 1465 1665 Size: 822 32 579 32 200 Fragments arranged by size: 822 579 200 32 32 HindIII A'AGCT_T Cuts at: 0 1434 1665 Size: 1434 231 KpnI G_GTAC'C Cuts at: 0 828 1398 1665 Size: 828 570 267 Fragments arranged by size: 828 570 267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1186 1665 Size: 1186 479 MscI TGG'CCA Cuts at: 0 998 1066 1665 Size: 998 68 599 Fragments arranged by size: 998 599 68 MslI CAynn'nnrTG Cuts at: 0 931 1018 1665 Size: 931 87 647 Fragments arranged by size: 931 647 87 MspA1I CmG'CkG Cuts at: 0 52 414 1665 Size: 52 362 1251 Fragments arranged by size: 1251 362 52 NcoI C'CATG_G Cuts at: 0 714 792 1665 Size: 714 78 873 Fragments arranged by size: 873 714 78 NsiI A_TGCA'T Cuts at: 0 1259 1665 Size: 1259 406 NspI r_CATG'y Cuts at: 0 1017 1107 1665 Size: 1017 90 558 Fragments arranged by size: 1017 558 90 PasI CC'CwG_GG Cuts at: 0 737 1665 Size: 737 928 PciI A'CATG_T Cuts at: 0 1103 1665 Size: 1103 562 PflMI CCAn_nnn'nTGG Cuts at: 0 374 1518 1665 Size: 374 1144 147 Fragments arranged by size: 1144 374 147 PfoI T'CCnGG_A Cuts at: 0 608 959 1665 Size: 608 351 706 Fragments arranged by size: 706 608 351 PpuMI rG'GwC_Cy Cuts at: 0 861 1381 1665 Size: 861 520 284 Fragments arranged by size: 861 520 284 PstI C_TGCA'G Cuts at: 0 214 1665 Size: 214 1451 PvuII CAG'CTG Cuts at: 0 414 1665 Size: 414 1251 SapI GCTCTTCn'nnn_ Cuts at: 0 1295 1665 Size: 1295 370 SfcI C'TryA_G Cuts at: 0 210 1665 Size: 210 1455 SgrAI Cr'CCGG_yG Cuts at: 0 1647 1665 Size: 1647 18 SmlI C'TyrA_G Cuts at: 0 980 1665 Size: 980 685 SphI G_CATG'C Cuts at: 0 1017 1665 Size: 1017 648 SspI AAT'ATT Cuts at: 0 922 1665 Size: 922 743 StyI C'CwwG_G Cuts at: 0 714 792 1665 Size: 714 78 873 Fragments arranged by size: 873 714 78 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 375 1006 1665 Size: 375 631 659 Fragments arranged by size: 659 631 375 XmnI GAAnn'nnTTC Cuts at: 0 346 1486 1665 Size: 346 1140 179 Fragments arranged by size: 1140 346 179 Enzymes that do cut: Acc65I AcuI AflIII AgeI AloI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BciVI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BstAPI BstEII BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI PstI PvuII SapI SfcI SgrAI SmlI SphI SspI StyI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BglI BlpI BmgBI BmtI BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SrfI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI