################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BbrPI BclI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I KasI Ksp632I KspI MaeIII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 184 GGTACC G^GTACC AclI 446 AACGTT AA^CGTT AfaI 185 GTAC GT^AC Alw44I 78 GTGCAC G^TGCAC AocI 784 CCTGAGG CC^T[ACGT]AGG ApaLI 78 GTGCAC G^TGCAC Asp718I 184 GGTACC G^GTACC AspI 808 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 977 CTCGGG C^[CT]CG[GA]G AvaII 90 GGTCC G^G[AT]CC BamHI 858 GGATCC G^GATCC BbsI 1194 GAAGAC GAAGAC(2/6) BcgI 61 CGACCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 832 GTATCC GTATCC(6/5) BcnI 83 CCGGG CC^[GC]GG BglII 953 AGATCT A^GATCT BlpI 45 GCTCAGC GC^T[ACGT]AGC BpmI 889 CTGGAG CTGGAG(16/14) Bpu1102I 45 GCTCAGC GC^T[ACGT]AGC BpuAI 1194 GAAGAC GAAGAC(2/6) BsaI 902 GAGACC GGTCTC(1/5) BsgI 1357 CTGCAC GTGCAG(16/14) BsiEI 61 CGACCG CG[GA][CT]^CG BsmI 1379 GCATTC GAATGC(1/-1) BsoBI 977 CTCGGG C^[CT]CG[GA]G BsrDI 534 CATTGC GCAATG(2/0) BsrFI 641 ACCGGC [GA]^CCGG[CT] BstXI 816 CCAGTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 784 CCTGAGG CC^T[ACGT]AGG CelII 45 GCTCAGC GC^T[ACGT]AGC Cfr10I 641 ACCGGC [GA]^CCGG[CT] CpoI 89 CGGTCCG CG^G[AT]CCG DraI 253 TTTAAA TTT^AAA DraIII 1126 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 784 CCTGAGG CC^T[ACGT]AGG HaeII 1085 GGCGCT [GA]GCGC^[CT] HincII 123 GTTAAC GT[CT]^[GA]AC HindII 123 GTTAAC GT[CT]^[GA]AC HindIII 1279 AAGCTT A^AGCTT HpaI 123 GTTAAC GTT^AAC KpnI 184 GGTACC GGTAC^C MslI 1360 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 83 CCGGG CC^[GC]GG NdeI 208 CATATG CA^TATG NspI 1106 GCATGT [GA]CATG^[CT] PflFI 808 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 446 AACGTT AA^CGTT PstI 1024 CTGCAG CTGCA^G RsaI 185 GTAC GT^AC RsrII 89 CGGTCCG CG^G[AT]CCG SmlI 1229 CTCAAG C^T[CT][GA]AG StuI 774 AGGCCT AGG^CCT TaqI 798 TCGA T^CGA Tth111I 808 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 798 TCGA T^CGA XcmI 816 CCAGTGTCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 52 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 883 CAGGCCCTG AspHI 78 GTGCAC G[AT]GC[AT]^C 1371 GAGCAC BanI 184 GGTACC G^G[CT][GA]CC 1131 GGTGCC BbvCI 202 CCTCAGC CCTCAGC(-5/-2) 966 CCTCAGC BsiHKAI 78 GTGCAC G[AT]GC[AT]^C 1371 GAGCAC CfoI 71 GCGC GCG^C 1086 GCGC EcoT14I 495 CCAAGG C^C[AT][AT]GG 1118 CCAAGG HapII 83 CCGG C^CGG 642 CCGG HhaI 71 GCGC GCG^C 1086 GCGC HinP1I 71 GCGC G^CGC 1086 GCGC HpaII 83 CCGG C^CGG 642 CCGG MaeII 258 ACGT A^CGT 447 ACGT MspA1I 246 CAGCTG C[AC]G^C[GT]G 1354 CAGCTG MspI 83 CCGG C^CGG 642 CCGG MwoI 45 GCTCAGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 71 GCGCTTCGTGC PvuII 246 CAGCTG CAG^CTG 1354 CAGCTG SfaNI 565 GATGC GCATC(5/9) 1320 GATGC SfcI 1024 CTGCAG C^T[GA][CT]AG 1220 CTACAG StyI 495 CCAAGG C^C[AT][AT]GG 1118 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BalI BanII BbvI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboI MboII MflI MluNI MnlI MscI MseI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI XhoII