(Circular) (Six-base) MAPSORT of: pFN21AE6447.seq Check: 3001 from: 1 to: 6223 pFN21AE6447 6223 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 1937 Size: 1629 4594 Acc65I G'GTAC_C Cuts at: 2189 3544 2189 Size: 1355 4868 AclI AA'CG_TT Cuts at: 1563 2452 4350 4723 1563 Size: 889 1898 373 3063 Fragments arranged by size: 3063 1898 889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2324 2378 4286 5670 5938 1632 Size: 692 54 1908 1384 268 1917 Fragments arranged by size: 1917 1908 1384 692 268 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 5122 6099 1203 Size: 3919 977 1327 Fragments arranged by size: 3919 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 3369 1166 Size: 2203 4020 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6223 AlwNI CAG_nnn'CTG Cuts at: 2062 2893 3966 5538 2062 Size: 831 1073 1572 2747 Fragments arranged by size: 2747 1572 1073 831 ApaLI G'TGCA_C Cuts at: 2083 4280 5436 2083 Size: 2197 1156 2870 Fragments arranged by size: 2870 2197 1156 ApoI r'AATT_y Cuts at: 1493 2164 2232 3484 3498 3532 3758 3910 6019 Size: 671 68 1252 14 34 226 152 2109 Cuts at: 6019 1493 Size: 1697 Fragments arranged by size: 2109 1697 1252 671 226 152 68 34 14 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2982 3548 1870 Size: 73 1039 566 4545 Fragments arranged by size: 4545 1039 566 73 BamHI G'GATC_C Cuts at: 2863 3553 3920 2863 Size: 690 367 5166 Fragments arranged by size: 5166 690 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2189 3136 3544 4999 618 Size: 325 217 627 402 947 408 1455 1842 Fragments arranged by size: 1842 1455 947 627 408 402 325 217 BanII G_rGCy'C Cuts at: 1391 2346 2489 3060 3542 1391 Size: 955 143 571 482 4072 Fragments arranged by size: 4072 955 571 482 143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3206 928 Size: 971 1307 3945 Fragments arranged by size: 3945 1307 971 BbvCI CC'TCA_GC Cuts at: 2208 2972 2208 Size: 764 5459 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2055 2089 4414 4448 1903 Size: 34 50 34 34 34 2325 34 3678 Fragments arranged by size: 3678 2325 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2848 4115 5325 2848 Size: 1267 1210 3746 Fragments arranged by size: 3746 1267 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4834 136 Size: 107 122 71 631 605 3162 1525 Fragments arranged by size: 3162 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2958 6218 2958 Size: 3260 2963 BlpI GC'TnA_GC Cuts at: 2051 3640 2051 Size: 1589 4634 Bme1580I G_kGCm'C Cuts at: 1790 2087 4284 5440 1790 Size: 297 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 297 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3954 4912 442 Size: 416 53 83 399 24 2537 958 1753 Fragments arranged by size: 2537 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2645 2645 Size: 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 4883 1239 Size: 1676 1968 2579 Fragments arranged by size: 2579 1968 1676 Bpu10I CC'TnA_GC Cuts at: 2208 2972 2208 Size: 764 5459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3219 4348 5213 5511 3219 Size: 1129 865 298 3931 Fragments arranged by size: 3931 1129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2901 4886 882 Size: 617 1402 1985 2219 Fragments arranged by size: 2219 1985 1402 617 BsaAI yAC'GTr Cuts at: 493 1204 4014 493 Size: 711 2810 2702 Fragments arranged by size: 2810 2702 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4030 4412 5047 275 Size: 53 83 186 936 2497 382 635 1451 Fragments arranged by size: 2497 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4656 5054 5328 5475 5886 4656 Size: 398 274 147 411 4993 Fragments arranged by size: 4993 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5998 Fragments arranged by size: 5998 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2704 3068 3476 1186 Size: 1518 364 408 3933 Fragments arranged by size: 3933 1518 408 364 BseYI C'CCAG_C Cuts at: 2290 3047 3357 5426 5922 2290 Size: 757 310 2069 496 2591 Fragments arranged by size: 2591 2069 757 496 310 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3347 1163 Size: 573 1611 4039 Fragments arranged by size: 4039 1611 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 3673 4434 5462 1540 Size: 275 186 68 1604 761 1028 2301 Fragments arranged by size: 2301 1604 1028 761 275 186 68 BsiHKAI G_wGCw'C Cuts at: 2087 3380 4284 4369 5440 2087 Size: 1293 904 85 1071 2870 Fragments arranged by size: 2870 1293 1071 904 85 BsmI GAATG_Cn' Cuts at: 3384 3734 3827 3384 Size: 350 93 5780 Fragments arranged by size: 5780 350 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2346 2489 3060 3380 3542 4284 Size: 399 297 259 143 571 320 162 742 Cuts at: 4284 4369 5440 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 399 320 297 259 162 143 85 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 4112 5842 4112 Size: 1730 4493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3578 844 Size: 2734 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4110 1394 Size: 429 2287 3507 Fragments arranged by size: 3507 2287 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2538 4718 4892 65 Size: 1183 1290 2180 174 1396 Fragments arranged by size: 2180 1396 1290 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2646 4867 5054 1700 Size: 110 836 2221 187 2869 Fragments arranged by size: 2869 2221 836 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 4283 5295 4283 Size: 1012 5211 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6223 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6223 BstXI CCAn_nnnn'nTGG Cuts at: 2828 2828 Size: 6223 BstYI r'GATC_y Cuts at: 1972 2197 2863 2958 3438 3553 3920 4305 4322 Size: 225 666 95 480 115 367 385 17 Cuts at: 4322 5774 5860 5872 6218 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 666 480 385 367 346 225 115 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2790 2790 Size: 6223 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5671 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 2130 3027 3079 3739 4533 4553 2130 Size: 897 52 660 794 20 3800 Fragments arranged by size: 3800 897 794 660 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 2260 3527 2260 Size: 1267 4956 DraIII CAC_nnn'GTG Cuts at: 3136 3136 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5230 809 Size: 507 3914 1802 Fragments arranged by size: 3914 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 3246 3450 Size: 54 1425 50 275 18 594 822 204 Cuts at: 3450 3468 3670 4559 6188 8 Size: 18 202 889 1629 43 Fragments arranged by size: 1629 1425 889 822 594 275 204 202 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4153 1353 Size: 2800 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2665 4810 5184 5330 387 Size: 1064 138 1076 2145 374 146 1280 Fragments arranged by size: 2145 1280 1076 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2378 2915 4286 4883 5670 5938 Size: 393 692 54 537 1371 597 787 268 Cuts at: 5938 1239 Size: 1524 Fragments arranged by size: 1524 1371 787 692 597 537 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2034 2890 2899 2986 2034 Size: 856 9 87 5271 Fragments arranged by size: 5271 856 87 9 EcoRI G'AATT_C Cuts at: 3532 3910 3532 Size: 378 5845 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2980 2980 Size: 6223 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 3094 5370 1678 Size: 1416 2276 2531 Fragments arranged by size: 2531 2276 1416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2945 2977 3025 Size: 32 135 32 794 32 547 32 48 Cuts at: 3025 3057 4864 4896 4938 4970 1373 Size: 32 1807 32 42 32 2626 Fragments arranged by size: 2626 1807 794 547 135 48 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2130 3567 3813 669 Size: 988 281 192 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 192 HindIII A'AGCT_T Cuts at: 748 3284 748 Size: 2536 3687 HpaI GTT'AAC Cuts at: 2130 3813 2130 Size: 1683 4540 KpnI G_GTAC'C Cuts at: 2193 3548 2193 Size: 1355 4868 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 5337 5521 1305 Size: 1138 2894 184 2007 Fragments arranged by size: 2894 2007 1138 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 3248 3452 3470 10 Size: 54 1425 343 594 822 204 18 2763 Fragments arranged by size: 2763 1425 822 594 343 204 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 3369 4181 4540 4699 518 Size: 648 2203 812 359 159 2042 Fragments arranged by size: 2203 2042 812 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2253 3361 3639 4316 5464 5709 1606 Size: 647 1108 278 677 1148 245 2120 Fragments arranged by size: 2120 1148 1108 677 647 278 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5671 NdeI CA'TA_TG Cuts at: 387 2214 387 Size: 1827 4396 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 3115 3581 5126 1132 Size: 97 1886 466 1545 2229 Fragments arranged by size: 2229 1886 1545 466 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6223 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6223 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PstI C_TGCA'G Cuts at: 830 3033 3575 830 Size: 2203 542 3478 Fragments arranged by size: 3478 2203 542 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 PvuII CAG'CTG Cuts at: 2253 3361 2253 Size: 1108 5115 RsrII CG'GwC_CG Cuts at: 2095 2095 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SbfI CC_TGCA'GG Cuts at: 3575 3575 Size: 6223 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 3029 3225 3571 4706 5387 5578 826 Size: 219 1984 196 346 1135 681 191 1471 Fragments arranged by size: 1984 1471 1135 681 346 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3234 4327 5228 5490 820 Size: 197 926 1291 1093 901 262 1553 Fragments arranged by size: 1553 1291 1093 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 2781 1517 Size: 1264 4959 StyI C'CwwG_G Cuts at: 513 1065 2500 3123 3662 513 Size: 552 1435 623 539 3074 Fragments arranged by size: 3074 1435 623 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4250 4267 4420 4605 5050 325 Size: 1255 2670 17 153 185 445 1498 Fragments arranged by size: 2670 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4469 6181 96 Size: 274 80 33 51 1443 2492 1712 138 Fragments arranged by size: 2492 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3370 3402 6183 6215 3370 Size: 32 2781 32 3378 Fragments arranged by size: 3378 2781 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2816 1316 Size: 1500 4723 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2828 2828 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6223 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 4352 4352 Size: 6223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI