(Linear) (Six-base) MAPSORT of: KIEE6447.seq Check: 5420 from: 1 to: 1518 KIEE6447.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. Acc65I G'GTAC_C Cuts at: 0 184 1518 Size: 184 1334 AclI AA'CG_TT Cuts at: 0 447 1518 Size: 447 1071 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 373 1518 Size: 319 54 1145 Fragments arranged by size: 1145 319 54 AleI CACnn'nnGTG Cuts at: 0 1364 1518 Size: 1364 154 AlwNI CAG_nnn'CTG Cuts at: 0 57 888 1518 Size: 57 831 630 Fragments arranged by size: 831 630 57 ApaLI G'TGCA_C Cuts at: 0 78 1518 Size: 78 1440 ApoI r'AATT_y Cuts at: 0 159 227 1479 1493 1518 Size: 159 68 1252 14 25 Fragments arranged by size: 1252 159 68 25 14 AvaI C'yCGr_G Cuts at: 0 977 1518 Size: 977 541 BamHI G'GATC_C Cuts at: 0 858 1518 Size: 858 660 BanI G'GyrC_C Cuts at: 0 184 1131 1518 Size: 184 947 387 Fragments arranged by size: 947 387 184 BanII G_rGCy'C Cuts at: 0 341 484 1055 1518 Size: 341 143 571 463 Fragments arranged by size: 571 463 341 143 BbsI GAAGACnn'nnnn_ Cuts at: 0 1201 1518 Size: 1201 317 BbvCI CC'TCA_GC Cuts at: 0 203 967 1518 Size: 203 764 551 Fragments arranged by size: 764 551 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 50 84 1518 Size: 50 34 1434 Fragments arranged by size: 1434 50 34 BciVI GTATCCnnnnn_n' Cuts at: 0 843 1518 Size: 843 675 BglII A'GATC_T Cuts at: 0 953 1518 Size: 953 565 BlpI GC'TnA_GC Cuts at: 0 46 1518 Size: 46 1472 Bme1580I G_kGCm'C Cuts at: 0 82 1518 Size: 82 1436 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 640 1518 Size: 640 878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1518 Size: 910 608 Bpu10I CC'TnA_GC Cuts at: 0 203 967 1518 Size: 203 764 551 Fragments arranged by size: 764 551 203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1214 1518 Size: 1214 304 BsaI GGTCTCn'nnnn_ Cuts at: 0 896 1518 Size: 896 622 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 699 1063 1471 1518 Size: 699 364 408 47 Fragments arranged by size: 699 408 364 47 BseYI C'CCAG_C Cuts at: 0 285 1042 1352 1518 Size: 285 757 310 166 Fragments arranged by size: 757 310 285 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1342 1518 Size: 1342 176 BsiEI CG_ry'CG Cuts at: 0 64 1518 Size: 64 1454 BsiHKAI G_wGCw'C Cuts at: 0 82 1375 1518 Size: 82 1293 143 Fragments arranged by size: 1293 143 82 BsmI GAATG_Cn' Cuts at: 0 1379 1518 Size: 1379 139 Bsp1286I G_dGCh'C Cuts at: 0 82 341 484 1055 1375 1518 Size: 82 259 143 571 320 143 Fragments arranged by size: 571 320 259 143 143 82 BsrDI GCAATG_nn' Cuts at: 0 533 1518 Size: 533 985 BsrFI r'CCGG_y Cuts at: 0 641 1518 Size: 641 877 BstXI CCAn_nnnn'nTGG Cuts at: 0 823 1518 Size: 823 695 BstYI r'GATC_y Cuts at: 0 192 858 953 1433 1518 Size: 192 666 95 480 85 Fragments arranged by size: 666 480 192 95 85 Bsu36I CC'TnA_GG Cuts at: 0 785 1518 Size: 785 733 BtsI GCAGTG_nn' Cuts at: 0 125 1022 1074 1518 Size: 125 897 52 444 Fragments arranged by size: 897 444 125 52 DraI TTT'AAA Cuts at: 0 255 1518 Size: 255 1263 DraIII CAC_nnn'GTG Cuts at: 0 1131 1518 Size: 1131 387 EaeI y'GGCC_r Cuts at: 0 419 1241 1445 1463 1518 Size: 419 822 204 18 55 Fragments arranged by size: 822 419 204 55 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 660 1518 Size: 660 858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 373 910 1518 Size: 319 54 537 608 Fragments arranged by size: 608 537 319 54 EcoO109I rG'GnC_Cy Cuts at: 0 29 885 894 981 1518 Size: 29 856 9 87 537 Fragments arranged by size: 856 537 87 29 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 975 1518 Size: 975 543 HaeII r_GCGC'y Cuts at: 0 1089 1518 Size: 1089 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 361 393 940 972 1020 1052 1518 Size: 361 32 547 32 48 32 466 Fragments arranged by size: 547 466 361 48 32 32 32 HincII GTy'rAC Cuts at: 0 125 1518 Size: 125 1393 HindIII A'AGCT_T Cuts at: 0 1279 1518 Size: 1279 239 HpaI GTT'AAC Cuts at: 0 125 1518 Size: 125 1393 KpnI G_GTAC'C Cuts at: 0 188 1518 Size: 188 1330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 438 1518 Size: 438 1080 MscI TGG'CCA Cuts at: 0 421 1243 1447 1465 1518 Size: 421 822 204 18 53 Fragments arranged by size: 822 421 204 53 18 MslI CAynn'nnrTG Cuts at: 0 1364 1518 Size: 1364 154 MspA1I CmG'CkG Cuts at: 0 248 1356 1518 Size: 248 1108 162 Fragments arranged by size: 1108 248 162 NdeI CA'TA_TG Cuts at: 0 209 1518 Size: 209 1309 NspI r_CATG'y Cuts at: 0 1110 1518 Size: 1110 408 PstI C_TGCA'G Cuts at: 0 1028 1518 Size: 1028 490 PvuII CAG'CTG Cuts at: 0 248 1356 1518 Size: 248 1108 162 Fragments arranged by size: 1108 248 162 RsrII CG'GwC_CG Cuts at: 0 90 1518 Size: 90 1428 SfcI C'TryA_G Cuts at: 0 1024 1220 1518 Size: 1024 196 298 Fragments arranged by size: 1024 298 196 SmlI C'TyrA_G Cuts at: 0 1229 1518 Size: 1229 289 StuI AGG'CCT Cuts at: 0 776 1518 Size: 776 742 StyI C'CwwG_G Cuts at: 0 495 1118 1518 Size: 495 623 400 Fragments arranged by size: 623 495 400 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1365 1397 1518 Size: 1365 32 121 Fragments arranged by size: 1365 121 32 Tth111I GACn'n_nGTC Cuts at: 0 811 1518 Size: 811 707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 823 1518 Size: 823 695 Enzymes that do cut: Acc65I AclI AcuI AleI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BstXI BstYI Bsu36I BtsI DraI DraIII EaeI EciI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NdeI NspI PstI PvuII RsrII SfcI SmlI StuI StyI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BclI BfrBI BglI BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI KasI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI XbaI XhoI XmaI XmnI ZraI