(Circular) (Six-base) MAPSORT of: pFN21AE1492.seq Check: 3670 from: 1 to: 7243 pFN21AE1492 7243 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5053 278 Size: 53 83 186 936 3517 2468 Fragments arranged by size: 3517 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4586 1937 Size: 2649 4594 Acc65I G'GTAC_C Cuts at: 2803 4564 2803 Size: 1761 5482 AclI AA'CG_TT Cuts at: 1563 5370 5743 1563 Size: 3807 373 3063 Fragments arranged by size: 3807 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2028 3366 3393 5306 6690 6958 1632 Size: 396 1338 27 1913 1384 268 1917 Fragments arranged by size: 1917 1913 1384 1338 396 268 27 AflII C'TTAA_G Cuts at: 820 1017 2306 820 Size: 197 1289 5757 Fragments arranged by size: 5757 1289 197 AflIII A'CryG_T Cuts at: 1203 2655 3083 6142 7119 1203 Size: 1452 428 3059 977 1327 Fragments arranged by size: 3059 1452 1327 977 428 AgeI A'CCGG_T Cuts at: 6074 6074 Size: 7243 AhdI GACnn_n'nnGTC Cuts at: 2683 3991 5972 2683 Size: 1308 1981 3954 Fragments arranged by size: 3954 1981 1308 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7243 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2734 3908 4359 1184 Size: 1550 1174 451 4068 Fragments arranged by size: 4068 1550 1174 451 AlwNI CAG_nnn'CTG Cuts at: 3038 3577 4986 6558 3038 Size: 539 1409 1572 3723 Fragments arranged by size: 3723 1572 1409 539 ApaLI G'TGCA_C Cuts at: 5300 6456 5300 Size: 1156 6087 ApoI r'AATT_y Cuts at: 1493 2186 3449 3779 4046 4552 4778 4930 7039 Size: 693 1263 330 267 506 226 152 2109 Cuts at: 7039 1493 Size: 1697 Fragments arranged by size: 2109 1697 1263 693 506 330 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 5797 160 Size: 872 4765 1606 Fragments arranged by size: 4765 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 AvaI C'yCGr_G Cuts at: 1870 1943 2349 4568 1870 Size: 73 406 2219 4545 Fragments arranged by size: 4545 2219 406 73 AvrII C'CTAG_G Cuts at: 3434 3434 Size: 7243 BamHI G'GATC_C Cuts at: 4573 4940 4573 Size: 367 6876 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 4564 6019 618 Size: 325 217 627 1016 1761 1455 1842 Fragments arranged by size: 1842 1761 1455 1016 627 325 217 BanII G_rGCy'C Cuts at: 1391 3875 4386 4562 1391 Size: 2484 511 176 4072 Fragments arranged by size: 4072 2484 511 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3332 928 Size: 971 1433 4839 Fragments arranged by size: 4839 1433 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2306 2340 5434 5468 1903 Size: 34 369 34 3094 34 3678 Fragments arranged by size: 3678 3094 369 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2004 2086 5135 6345 2004 Size: 82 3049 1210 2902 Fragments arranged by size: 3049 2902 1210 82 BclI T'GATC_A Cuts at: 1548 2590 3715 1548 Size: 1042 1125 5076 Fragments arranged by size: 5076 1125 1042 BfrBI ATG'CAT Cuts at: 4070 4070 Size: 7243 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5854 136 Size: 107 122 71 631 605 4182 1525 Fragments arranged by size: 4182 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2273 7238 2273 Size: 4965 2278 BlpI GC'TnA_GC Cuts at: 4301 4660 4301 Size: 359 6884 Bme1580I G_kGCm'C Cuts at: 1790 5304 6460 1790 Size: 3514 1156 2573 Fragments arranged by size: 3514 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7243 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4445 4974 5932 Size: 416 53 83 399 24 3028 529 958 Cuts at: 5932 442 Size: 1753 Fragments arranged by size: 3028 1753 958 529 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7243 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3778 3894 3778 Size: 116 7127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3861 4461 5903 1239 Size: 2622 600 1442 2579 Fragments arranged by size: 2622 2579 1442 600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2771 5368 6233 6531 2771 Size: 2597 865 298 3483 Fragments arranged by size: 3483 2597 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2424 3627 5906 882 Size: 617 925 1203 2279 2219 Fragments arranged by size: 2279 2219 1203 925 617 BsaAI yAC'GTr Cuts at: 493 1204 3674 5034 493 Size: 711 2470 1360 2702 Fragments arranged by size: 2702 2470 1360 711 BsaBI GATnn'nnATC Cuts at: 2795 2919 7237 2795 Size: 124 4318 2801 Fragments arranged by size: 4318 2801 124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5050 5432 6067 275 Size: 53 83 186 936 3517 382 635 1451 Fragments arranged by size: 3517 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5676 6074 6348 6495 6906 5676 Size: 398 274 147 411 6013 Fragments arranged by size: 6013 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4145 4175 1178 Size: 30 165 30 2742 30 4246 Fragments arranged by size: 4246 2742 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3989 1186 Size: 2803 4440 BseYI C'CCAG_C Cuts at: 2942 6446 6942 2942 Size: 3504 496 3243 Fragments arranged by size: 3504 3243 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2632 3291 3510 1163 Size: 573 896 659 219 4896 Fragments arranged by size: 4896 896 659 573 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4693 5454 6482 1540 Size: 275 186 2692 761 1028 2301 Fragments arranged by size: 2692 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4091 4386 5304 5389 6460 4091 Size: 295 918 85 1071 4874 Fragments arranged by size: 4874 1071 918 295 85 BsmI GAATG_Cn' Cuts at: 4754 4847 4754 Size: 93 7150 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3875 4091 4386 4562 5304 5389 6460 Size: 399 2085 216 295 176 742 85 1071 Cuts at: 6460 1391 Size: 2174 Fragments arranged by size: 2174 2085 1071 742 399 295 216 176 85 BspEI T'CCGG_A Cuts at: 6906 6906 Size: 7243 BspHI T'CATG_A Cuts at: 5132 6862 5132 Size: 1730 5513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2068 3507 4291 4598 844 Size: 1224 1439 784 307 3489 Fragments arranged by size: 3489 1439 1224 784 307 BsrBI CCG'CTC Cuts at: 1394 1823 5130 1394 Size: 429 3307 3507 Fragments arranged by size: 3507 3307 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3890 5738 5912 65 Size: 1183 2642 1848 174 1396 Fragments arranged by size: 2642 1848 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 5887 6074 1700 Size: 110 2502 1575 187 2869 Fragments arranged by size: 2869 2502 1575 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7243 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7243 BssSI C'ACGA_G Cuts at: 2438 5303 6315 2438 Size: 2865 1012 3366 Fragments arranged by size: 3366 2865 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7243 BstBI TT'CG_AA Cuts at: 6047 6047 Size: 7243 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7243 BstXI CCAn_nnnn'nTGG Cuts at: 4040 4040 Size: 7243 BstYI r'GATC_y Cuts at: 1972 2273 2521 4573 4940 5325 5342 6794 6880 Size: 301 248 2052 367 385 17 1452 86 Cuts at: 6880 6892 7238 1972 Size: 12 346 1977 Fragments arranged by size: 2052 1977 1452 385 367 346 301 248 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2388 513 Size: 552 939 384 5368 Fragments arranged by size: 5368 939 552 384 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6504 Fragments arranged by size: 6504 683 56 BtsI GCAGTG_nn' Cuts at: 2690 3036 4052 4759 5553 5573 2690 Size: 346 1016 707 794 20 4360 Fragments arranged by size: 4360 1016 794 707 346 20 ClaI AT'CG_AT Cuts at: 1554 3018 1554 Size: 1464 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3174 3209 3789 3824 529 Size: 35 2610 35 580 35 3948 Fragments arranged by size: 3948 2610 580 35 35 35 DraI TTT'AAA Cuts at: 2556 4405 4547 2556 Size: 1849 142 5252 Fragments arranged by size: 5252 1849 142 DraIII CAC_nnn'GTG Cuts at: 2696 2696 Size: 7243 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6250 809 Size: 507 4934 1802 Fragments arranged by size: 4934 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4516 4690 5579 Size: 54 1425 50 275 18 2686 174 889 Cuts at: 5579 7208 8 Size: 1629 43 Fragments arranged by size: 2686 1629 1425 889 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4690 1537 Size: 275 2878 4090 Fragments arranged by size: 4090 2878 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2275 2634 2976 3747 4322 4482 5173 1353 Size: 922 359 342 771 575 160 691 3423 Fragments arranged by size: 3423 922 771 691 575 359 342 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5830 6204 6350 387 Size: 1064 138 4241 374 146 1280 Fragments arranged by size: 4241 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4384 4384 Size: 7243 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 3366 3393 3861 4461 5306 5903 Size: 393 396 1338 27 468 600 845 597 Cuts at: 5903 6690 6958 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1338 845 787 600 597 468 396 393 268 27 EcoO109I rG'GnC_Cy Cuts at: 4280 4280 Size: 7243 EcoRI G'AATT_C Cuts at: 4046 4552 4930 4046 Size: 506 378 6359 Fragments arranged by size: 6359 506 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2318 2318 Size: 7243 FspI TGC'GCA Cuts at: 2463 5749 2463 Size: 3286 3957 HaeII r_GCGC'y Cuts at: 1678 6390 1678 Size: 4712 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 2769 2801 3536 Size: 32 135 32 1097 32 68 32 735 Cuts at: 3536 3568 3740 3772 3977 4009 4145 4177 4258 Size: 32 172 32 205 32 136 32 81 Cuts at: 4258 4290 4321 4353 4449 4481 5884 5916 5958 Size: 32 31 32 96 32 1403 32 42 Cuts at: 5958 5990 1373 Size: 32 2626 Fragments arranged by size: 2626 1403 1097 735 205 172 136 135 96 81 68 42 32 32 32 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 3658 4587 4833 669 Size: 988 281 1720 929 246 3079 Fragments arranged by size: 3079 1720 988 929 281 246 HindIII A'AGCT_T Cuts at: 748 2040 4076 4421 748 Size: 1292 2036 345 3570 Fragments arranged by size: 3570 2036 1292 345 HpaI GTT'AAC Cuts at: 4833 4833 Size: 7243 KpnI G_GTAC'C Cuts at: 2807 4568 2807 Size: 1761 5482 MfeI C'AATT_G Cuts at: 3170 4842 3170 Size: 1672 5571 MluI A'CGCG_T Cuts at: 7119 7119 Size: 7243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4492 6357 6541 1305 Size: 3187 1865 184 2007 Fragments arranged by size: 3187 2007 1865 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5421 Fragments arranged by size: 5421 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3041 4341 5201 5560 5719 518 Size: 648 891 984 1300 860 359 159 2042 Fragments arranged by size: 2042 1300 984 891 860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4659 5336 6484 6729 1606 Size: 3053 677 1148 245 2120 Fragments arranged by size: 3053 2120 1148 677 245 NaeI GCC'GGC Cuts at: 4314 4314 Size: 7243 NcoI C'CATG_G Cuts at: 513 1065 2004 2388 513 Size: 552 939 384 5368 Fragments arranged by size: 5368 939 552 384 NdeI CA'TA_TG Cuts at: 387 387 Size: 7243 NgoMIV G'CCGG_C Cuts at: 4312 4312 Size: 7243 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7243 NotI GC'GGCC_GC Cuts at: 4690 4690 Size: 7243 NsiI A_TGCA'T Cuts at: 4072 4072 Size: 7243 NspI r_CATG'y Cuts at: 1132 1229 2387 2659 2721 3087 4020 4601 6146 Size: 97 1158 272 62 366 933 581 1545 Cuts at: 6146 1132 Size: 2229 Fragments arranged by size: 2229 1545 1158 933 581 366 272 97 62 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7243 PciI A'CATG_T Cuts at: 2655 3083 6142 2655 Size: 428 3059 3756 Fragments arranged by size: 3756 3059 428 PflMI CCAn_nnn'nTGG Cuts at: 1108 3577 1108 Size: 2469 4774 PmeI GTTT'AAAC Cuts at: 4547 4547 Size: 7243 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5312 5344 6825 6857 5312 Size: 32 1481 32 5698 Fragments arranged by size: 5698 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4280 4280 Size: 7243 PshAI GACnn'nnGTC Cuts at: 1613 3142 1613 Size: 1529 5714 PsiI TTA'TAA Cuts at: 4813 4813 Size: 7243 PstI C_TGCA'G Cuts at: 830 4059 4246 4595 830 Size: 3229 187 349 3478 Fragments arranged by size: 3478 3229 349 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7243 SacI G_AGCT'C Cuts at: 4386 4386 Size: 7243 SalI G'TCGA_C Cuts at: 1936 4585 1936 Size: 2649 4594 SapI GCTCTTCn'nnn_ Cuts at: 2976 2976 Size: 7243 SbfI CC_TGCA'GG Cuts at: 4595 4595 Size: 7243 ScaI AGT'ACT Cuts at: 3190 3217 5491 7203 3190 Size: 27 2274 1712 3230 Fragments arranged by size: 3230 2274 1712 27 SexAI A'CCwGG_T Cuts at: 3390 3390 Size: 7243 SfcI C'TryA_G Cuts at: 826 1045 4055 4242 4591 5726 6407 6598 826 Size: 219 3010 187 349 1135 681 191 1471 Fragments arranged by size: 3010 1471 1135 681 349 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7243 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7243 SmaI CCC'GGG Cuts at: 1872 4570 1872 Size: 2698 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2349 2786 5347 6248 6510 Size: 197 926 363 43 437 2561 901 262 Cuts at: 6510 820 Size: 1553 Fragments arranged by size: 2561 1553 926 901 437 363 262 197 43 SnaBI TAC'GTA Cuts at: 493 5034 493 Size: 4541 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7243 SphI G_CATG'C Cuts at: 1132 2721 4601 1132 Size: 1589 1880 3774 Fragments arranged by size: 3774 1880 1589 SspI AAT'ATT Cuts at: 5 52 4129 4536 5167 5 Size: 47 4077 407 631 2081 Fragments arranged by size: 4077 2081 631 407 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7243 StyI C'CwwG_G Cuts at: 513 1065 2004 2388 3236 3434 4682 513 Size: 552 939 384 848 198 1248 3074 Fragments arranged by size: 3074 1248 939 848 552 384 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3817 5270 5287 5440 5625 6070 325 Size: 1255 2237 1453 17 153 185 445 1498 Fragments arranged by size: 2237 1498 1453 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2243 3188 3215 Size: 274 80 33 51 1443 266 945 27 Cuts at: 3215 5489 7201 96 Size: 2274 1712 138 Fragments arranged by size: 2274 1712 1443 945 274 266 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7203 7235 7203 Size: 32 7211 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7243 XbaI T'CTAG_A Cuts at: 2276 4579 2276 Size: 2303 4940 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2573 2573 Size: 7243 XhoI C'TCGA_G Cuts at: 1943 2349 1943 Size: 406 6837 XmaI C'CCGG_G Cuts at: 1870 4568 1870 Size: 2698 4545 XmnI GAAnn'nnTTC Cuts at: 4422 5372 4422 Size: 950 6293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5051 276 Size: 53 83 186 936 3517 2468 Fragments arranged by size: 3517 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI