################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I ClaI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 36 GGCGTC G[GA]^CG[CT]C AlwNI 67 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 185 CCCGGG C^[CT]CG[GA]G BbvCI 534 CCTCAGC CCTCAGC(-5/-2) BcgI 14 GCAAGGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 403 GCTCAGC GC^T[ACGT]AGC Bpu1102I 403 GCTCAGC GC^T[ACGT]AGC BsaAI 127 TACGTG [CT]AC^GT[GA] BsaHI 36 GGCGTC G[GA]^CG[CT]C BsmBI 109 GAGACG CGTCTC(1/5) BsoBI 185 CCCGGG C^[CT]CG[GA]G BspMI 165 GCAGGT ACCTGC(4/8) BsrFI 458 ACCGGC [GA]^CCGG[CT] BsrI 410 ACTGG ACTGG(1/-1) BstEII 336 GGTCACC G^GT[ACGT]ACC BstF5I 567 GGATG GGATG(2/0) BstPI 336 GGTCACC G^GT[ACGT]ACC Cac8I 493 GCCAGC GC[ACGT]^[ACGT]GC CelII 403 GCTCAGC GC^T[ACGT]AGC CfoI 462 GCGC GCG^C Cfr10I 458 ACCGGC [GA]^CCGG[CT] CpoI 455 CGGACCG CG^G[AT]CCG DpnII 105 GATC ^GATC EarI 6 GAAGAG CTCTTC(1/4) EcoO65I 336 GGTCACC G^GT[ACGT]ACC FokI 567 GGATG GGATG(9/13) HaeII 461 GGCGCT [GA]GCGC^[CT] HhaI 462 GCGC GCG^C Hin1I 36 GGCGTC G[GA]^CG[CT]C HinP1I 462 GCGC G^CGC HinfI 274 GAGTC G^A[ACGT]TC Ksp632I 6 GAAGAG CTCTTC(1/4) MboI 105 GATC ^GATC NdeII 105 GATC ^GATC NlaIII 85 CATG CATG^ PflMI 84 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 274 GAGTC GAGTC(4/5) RsrII 455 CGGACCG CG^G[AT]CCG SapI 6 GAAGAGC GCTCTTC(1/4) Sau3AI 105 GATC ^GATC SfcI 255 CTATAG C^T[GA][CT]AG SmaI 185 CCCGGG CCC^GGG SmlI 298 CTCAAG C^T[CT][GA]AG TaqI 107 TCGA T^CGA TthHB8I 107 TCGA T^CGA Van91I 84 CCATGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 185 CCCGGG C^CCGGG XmaI 185 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 388 GTAGAC GT^[AC][GT]AC 484 GTCTAC AvaII 348 GGTCC G^G[AT]CC 456 GGACC BalI 30 TGGCCA TGG^CCA 491 TGGCCA BpmI 61 CTGGAG CTGGAG(16/14) 472 CTGGAG BsmAI 109 GAGAC GTCTC(1/5) 142 GAGAC DdeI 404 CTCAG C^T[ACGT]AG 535 CTCAG EaeI 30 TGGCCA [CT]^GGCC[GA] 491 TGGCCA HphI 429 GGTGA GGTGA(8/7) 338 TCACC MaeII 128 ACGT A^CGT 245 ACGT MaeIII 337 GTCAC ^GT[ACGT]AC 365 GTGAC MboII 6 GAAGA GAAGA(8/7) 543 GAAGA MluNI 30 TGGCCA TGG^CCA 491 TGGCCA MscI 30 TGGCCA TGG^CCA 491 TGGCCA MslI 123 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 339 CACCATCGTG MspA1I 379 CAGCGG C[AC]G^C[GT]G 555 CAGCGG Tsp45I 337 GTCAC ^GT[GC]AC 365 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I EcoRII EcoT14I Fnu4HI HaeIII HapII HgaI HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI StyI TseI TspRI