Restriction map for
pFN21AB9441
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BbrPI BclI BfrI BglI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1702 GTCTAC GT^[AC][GT]AC
AcsI 259 GAATTT [GA]^AATT[CT]
AcyI 1456 GGCGCC G[GA]^CG[CT]C
AocI 1423 CCTAAGG CC^T[ACGT]AGG
ApaI 1127 GGGCCC GGGCC^C
ApoI 259 GAATTT [GA]^AATT[CT]
AseI 1650 ATTAAT AT^TAAT
AsnI 1650 ATTAAT AT^TAAT
AvaI 410 CTCGGG C^[CT]CG[GA]G
BamHI 1401 GGATCC G^GATCC
BanI 1456 GGCGCC G^G[CT][GA]CC
BbeI 1456 GGCGCC GGCGC^C
BbvCI 1343 CCTCAGC CCTCAGC(-5/-2)
BciVI 1644 GGATAC GTATCC(6/5)
BglII 1537 AGATCT A^GATCT
BsaHI 1456 GGCGCC G[GA]^CG[CT]C
BsaI 1055 GGTCTC GGTCTC(1/5)
BsoBI 410 CTCGGG C^[CT]CG[GA]G
Bsp1407I 104 TGTACA T^GTACA
BspHI 1695 TCATGA T^CATGA
BspMI 1710 GCAGGT ACCTGC(4/8)
BsrDI 1246 GCAATG GCAATG(2/0)
BsrGI 104 TGTACA T^GTACA
BstXI 432 CCAGGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1423 CCTAAGG CC^T[ACGT]AGG
DsaI 192 CCATGG C^C[GA][CT]GG
Eco81I 1423 CCTAAGG CC^T[ACGT]AGG
Hin1I 1456 GGCGCC G[GA]^CG[CT]C
HincII 1197 GTTGAC GT[CT]^[GA]AC
HindII 1197 GTTGAC GT[CT]^[GA]AC
HindIII 300 AAGCTT A^AGCTT
KasI 1456 GGCGCC G^GCGCC
MaeII 1139 ACGT A^CGT
MseI 1651 TTAA T^TAA
NarI 1456 GGCGCC GG^CGCC
NcoI 192 CCATGG C^CATGG
NheI 1281 GCTAGC G^CTAGC
NspI 915 GCATGC [GA]CATG^[CT]
PflMI 125 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 154 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1049 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1650 ATTAAT AT^TAAT
RcaI 1695 TCATGA T^CATGA
SfoI 1456 GGCGCC GGC^GCC
SmlI 1549 CTTGAG C^T[CT][GA]AG
SphI 915 GCATGC GCATG^C
SspBI 104 TGTACA T^GTACA
Tru9I 1651 TTAA T^TAA
Van91I 125 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 591 TCTAGA T^CTAGA
XcmI 461 CCATCGACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 105 GTAC GT^AC
1688 GTAC
AlwNI 1076 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1711 CAGGTTCTG
Asp700I 274 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1607 GAAAGCCTTC
AvaII 155 GGACC G^G[AT]CC
1545 GGACC
BcgI 298 GCAAGCTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
402 GCAAGCTGCTCG
BlpI 480 GCTGAGC GC^T[ACGT]AGC
1086 GCTAAGC
Bpu1102I 480 GCTGAGC GC^T[ACGT]AGC
1086 GCTAAGC
BsgI 983 CTGCAC GTGCAG(16/14)
1417 GTGCAG
BstYI 1401 GGATCC [GA]^GATC[CT]
1537 AGATCT
CelII 480 GCTGAGC GC^T[ACGT]AGC
1086 GCTAAGC
CfoI 7 GCGC GCG^C
1457 GCGC
EarI 379 GAAGAG CTCTTC(1/4)
658 GAAGAG
HaeII 6 GGCGCT [GA]GCGC^[CT]
1456 GGCGCC
HhaI 7 GCGC GCG^C
1457 GCGC
HinP1I 7 GCGC G^CGC
1457 GCGC
Ksp632I 379 GAAGAG CTCTTC(1/4)
658 GAAGAG
MflI 1401 GGATCC [GA]^GATC[CT]
1537 AGATCT
PstI 444 CTGCAG CTGCA^G
1484 CTGCAG
RsaI 105 GTAC GT^AC
1688 GTAC
SfcI 444 CTGCAG C^T[GA][CT]AG
1484 CTGCAG
Tsp509I 260 AATT ^AATT
763 AATT
XhoII 1401 GGATCC [GA]^GATC[CT]
1537 AGATCT
XmnI 274 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1607 GAAAGCCTTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I