(Circular) (Six-base) MAPSORT of: pFN21AB9441.seq Check: 2857 from: 1 to: 6451 pFN21AB9441 6451 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4261 278 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3708 3794 1937 Size: 1771 86 4594 Fragments arranged by size: 4594 1771 86 Acc65I G'GTAC_C Cuts at: 3772 3772 Size: 6451 AclI AA'CG_TT Cuts at: 1563 4578 4951 1563 Size: 3015 373 3063 Fragments arranged by size: 3063 3015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2997 3112 3368 3432 3483 4514 5898 Size: 440 925 115 256 64 51 1031 1384 Cuts at: 5898 6166 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1031 925 440 268 256 115 64 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6254 AflIII A'CryG_T Cuts at: 1203 5350 6327 1203 Size: 4147 977 1327 Fragments arranged by size: 4147 1327 977 AgeI A'CCGG_T Cuts at: 5282 5282 Size: 6451 AhdI GACnn_n'nnGTC Cuts at: 5180 5180 Size: 6451 AleI CACnn'nnGTG Cuts at: 1166 2876 3491 1166 Size: 1710 615 4126 Fragments arranged by size: 4126 1710 615 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3044 3386 1184 Size: 1860 342 4249 Fragments arranged by size: 4249 1860 342 AlwNI CAG_nnn'CTG Cuts at: 3086 3721 4194 5766 3086 Size: 635 473 1572 3771 Fragments arranged by size: 3771 1572 635 473 ApaI G_GGCC'C Cuts at: 3136 3136 Size: 6451 ApaLI G'TGCA_C Cuts at: 4508 5664 4508 Size: 1156 5295 ApoI r'AATT_y Cuts at: 1493 2264 3760 3986 4138 6247 1493 Size: 771 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 771 226 152 AseI AT'TA_AT Cuts at: 160 1032 3656 5005 160 Size: 872 2624 1349 1606 Fragments arranged by size: 2624 1606 1349 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 AvaI C'yCGr_G Cuts at: 1870 1943 2415 3776 1870 Size: 73 472 1361 4545 Fragments arranged by size: 4545 1361 472 73 BamHI G'GATC_C Cuts at: 3406 3781 4148 3406 Size: 375 367 5709 Fragments arranged by size: 5709 375 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3461 3772 5227 618 Size: 325 217 627 1674 311 1455 1842 Fragments arranged by size: 1842 1674 1455 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2766 2809 2951 3125 3136 3770 1391 Size: 1375 43 142 174 11 634 4072 Fragments arranged by size: 4072 1375 634 174 142 43 11 BbeI G_GCGC'C Cuts at: 3465 3465 Size: 6451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 3047 3489 928 Size: 971 251 897 442 3890 Fragments arranged by size: 3890 971 897 442 251 BbvCI CC'TCA_GC Cuts at: 3349 3349 Size: 6451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2292 2326 2396 2430 4642 4676 1903 Size: 34 355 34 70 34 2212 34 3678 Fragments arranged by size: 3678 2212 355 70 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3643 4343 5553 3643 Size: 700 1210 4541 Fragments arranged by size: 4541 1210 700 BclI T'GATC_A Cuts at: 1548 1548 Size: 6451 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5062 136 Size: 107 122 71 631 605 337 3053 1525 Fragments arranged by size: 3053 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3542 6446 3542 Size: 2904 3547 BlpI GC'TnA_GC Cuts at: 2486 3092 3868 2486 Size: 606 776 5069 Fragments arranged by size: 5069 776 606 Bme1580I G_kGCm'C Cuts at: 1790 3136 4512 5668 1790 Size: 1346 1376 1156 2573 Fragments arranged by size: 2573 1376 1346 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4182 5140 442 Size: 416 53 83 399 24 2765 958 1753 Fragments arranged by size: 2765 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3290 1056 Size: 2234 4217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3334 3334 Size: 6451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 2524 2874 3173 3629 5111 1239 Size: 1259 26 350 299 456 1482 2579 Fragments arranged by size: 2579 1482 1259 456 350 299 26 Bpu10I CC'TnA_GC Cuts at: 3087 3349 3359 3087 Size: 262 10 6179 Fragments arranged by size: 6179 262 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3575 4576 5441 5739 3575 Size: 1001 865 298 4287 Fragments arranged by size: 4287 1001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3066 5114 882 Size: 617 1567 2048 2219 Fragments arranged by size: 2219 2048 1567 617 BsaAI yAC'GTr Cuts at: 493 1204 4242 493 Size: 711 3038 2702 Fragments arranged by size: 3038 2702 711 BsaBI GATnn'nnATC Cuts at: 6445 6445 Size: 6451 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3462 4258 4640 5275 Size: 53 83 186 936 1929 796 382 635 Cuts at: 5275 275 Size: 1451 Fragments arranged by size: 1929 1451 936 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4884 5282 5556 5703 6114 4884 Size: 398 274 147 411 5221 Fragments arranged by size: 5221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6226 Fragments arranged by size: 6226 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2817 3570 1186 Size: 1216 415 753 4067 Fragments arranged by size: 4067 1216 753 415 BseYI C'CCAG_C Cuts at: 2024 2403 2432 3002 5654 6150 2024 Size: 379 29 570 2652 496 2325 Fragments arranged by size: 2652 2325 570 496 379 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2973 3443 1163 Size: 573 1237 470 4171 Fragments arranged by size: 4171 1237 573 470 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3901 4662 5690 1540 Size: 275 186 1900 761 1028 2301 Fragments arranged by size: 2301 1900 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4512 4597 5668 4512 Size: 85 1071 5295 Fragments arranged by size: 5295 1071 85 BsmI GAATG_Cn' Cuts at: 3962 4055 3962 Size: 93 6358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2766 2809 2951 3125 3136 3770 4512 Size: 399 976 43 142 174 11 634 742 Cuts at: 4512 4597 5668 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 976 742 634 399 174 142 85 43 11 BspEI T'CCGG_A Cuts at: 6114 6114 Size: 6451 BspHI T'CATG_A Cuts at: 3700 4340 6070 3700 Size: 640 1730 4081 Fragments arranged by size: 4081 1730 640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3706 3806 844 Size: 2862 100 3489 Fragments arranged by size: 3489 2862 100 BsrBI CCG'CTC Cuts at: 1394 1823 4338 1394 Size: 429 2515 3507 Fragments arranged by size: 3507 2515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3258 4946 5120 65 Size: 1183 2010 1688 174 1396 Fragments arranged by size: 2010 1688 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5095 5282 1700 Size: 110 3285 187 2869 Fragments arranged by size: 3285 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2109 96 Size: 2013 4438 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6451 BssSI C'ACGA_G Cuts at: 4511 5523 4511 Size: 1012 5439 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6451 BstBI TT'CG_AA Cuts at: 5255 5255 Size: 6451 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6451 BstXI CCAn_nnnn'nTGG Cuts at: 2444 2444 Size: 6451 BstYI r'GATC_y Cuts at: 1972 3406 3542 3781 4148 4533 4550 6002 6088 Size: 1434 136 239 367 385 17 1452 86 Cuts at: 6088 6100 6446 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1434 385 367 346 239 136 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3429 3429 Size: 6451 BtgI C'CryG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 4767 Fragments arranged by size: 4767 939 552 193 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2065 487 Size: 683 56 839 4873 Fragments arranged by size: 4873 839 683 56 BtsI GCAGTG_nn' Cuts at: 3042 3486 3498 3967 4761 4781 3042 Size: 444 12 469 794 20 4712 Fragments arranged by size: 4712 794 469 444 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6416 DraI TTT'AAA Cuts at: 3755 3755 Size: 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5458 809 Size: 507 4142 1802 Fragments arranged by size: 4142 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3898 4787 6416 Size: 54 1425 50 275 18 2068 889 1629 Cuts at: 6416 8 Size: 43 Fragments arranged by size: 2068 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3898 1537 Size: 275 2086 4090 Fragments arranged by size: 4090 2086 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2658 4381 1353 Size: 1026 279 1723 3423 Fragments arranged by size: 3423 1723 1026 279 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5038 5412 5558 387 Size: 1064 138 3449 374 146 1280 Fragments arranged by size: 3449 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2498 2524 2874 2997 3112 3173 Size: 393 440 426 26 350 123 115 61 Cuts at: 3173 3368 3432 3483 3629 4514 5111 5898 6166 Size: 195 64 51 146 885 597 787 268 Cuts at: 6166 1239 Size: 1524 Fragments arranged by size: 1524 885 787 597 440 426 393 350 268 195 146 123 115 64 61 51 26 EcoO109I rG'GnC_Cy Cuts at: 2160 3132 3133 2160 Size: 972 1 5478 Fragments arranged by size: 5478 972 1 EcoRI G'AATT_C Cuts at: 3760 4138 3760 Size: 378 6073 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2202 2202 Size: 6451 FspI TGC'GCA Cuts at: 4957 4957 Size: 6451 HaeII r_GCGC'y Cuts at: 1678 2015 3465 5598 1678 Size: 337 1450 2133 2531 Fragments arranged by size: 2531 2133 1450 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5092 5124 5166 5198 1373 Size: 32 135 32 3520 32 42 32 2626 Fragments arranged by size: 3520 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3795 4041 669 Size: 988 281 1266 591 246 3079 Fragments arranged by size: 3079 1266 988 591 281 246 HindIII A'AGCT_T Cuts at: 748 2305 748 Size: 1557 4894 HpaI GTT'AAC Cuts at: 4041 4041 Size: 6451 KasI G'GCGC_C Cuts at: 3461 3461 Size: 6451 KpnI G_GTAC'C Cuts at: 3776 3776 Size: 6451 MfeI C'AATT_G Cuts at: 4050 4050 Size: 6451 MluI A'CGCG_T Cuts at: 6327 6327 Size: 6451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2951 5565 5749 1305 Size: 1646 2614 184 2007 Fragments arranged by size: 2614 2007 1646 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4629 Fragments arranged by size: 4629 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3026 3491 4409 4768 4927 518 Size: 648 1710 150 465 918 359 159 2042 Fragments arranged by size: 2042 1710 918 648 465 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2975 3158 3278 3867 4544 5692 5937 1606 Size: 1369 183 120 589 677 1148 245 2120 Fragments arranged by size: 2120 1369 1148 677 589 245 183 120 NarI GG'CG_CC Cuts at: 3462 3462 Size: 6451 NcoI C'CATG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 4767 Fragments arranged by size: 4767 939 552 193 NdeI CA'TA_TG Cuts at: 387 387 Size: 6451 NheI G'CTAG_C Cuts at: 1052 3286 1052 Size: 2234 4217 NotI GC'GGCC_GC Cuts at: 3898 3898 Size: 6451 NspI r_CATG'y Cuts at: 1132 1229 2924 3809 5354 1132 Size: 97 1695 885 1545 2229 Fragments arranged by size: 2229 1695 1545 885 97 PasI CC'CwG_GG Cuts at: 1348 2546 2824 1348 Size: 1198 278 4975 Fragments arranged by size: 4975 1198 278 PciI A'CATG_T Cuts at: 5350 5350 Size: 6451 PflMI CCAn_nnn'nTGG Cuts at: 1108 2136 1108 Size: 1028 5423 PfoI T'CCnGG_A Cuts at: 2475 3645 2475 Size: 1170 5281 PmeI GTTT'AAAC Cuts at: 3755 3755 Size: 6451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4520 4552 6033 6065 4520 Size: 32 1481 32 4906 Fragments arranged by size: 4906 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2160 2160 Size: 6451 PshAI GACnn'nnGTC Cuts at: 1613 3058 1613 Size: 1445 5006 PsiI TTA'TAA Cuts at: 4021 4021 Size: 6451 PspOMI G'GGCC_C Cuts at: 3132 3132 Size: 6451 PstI C_TGCA'G Cuts at: 830 2453 3493 3803 830 Size: 1623 1040 310 3478 Fragments arranged by size: 3478 1623 1040 310 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6451 PvuII CAG'CTG Cuts at: 2975 3158 3278 2975 Size: 183 120 6148 Fragments arranged by size: 6148 183 120 SalI G'TCGA_C Cuts at: 1936 3793 1936 Size: 1857 4594 SbfI CC_TGCA'GG Cuts at: 3803 3803 Size: 6451 ScaI AGT'ACT Cuts at: 4699 6411 4699 Size: 1712 4739 SfcI C'TryA_G Cuts at: 826 1045 2449 3489 3799 4934 5615 5806 826 Size: 219 1404 1040 310 1135 681 191 1471 Fragments arranged by size: 1471 1404 1135 1040 681 310 219 191 SfoI GGC'GCC Cuts at: 3463 3463 Size: 6451 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6451 SmaI CCC'GGG Cuts at: 1872 3778 1872 Size: 1906 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3554 4555 5456 5718 820 Size: 197 926 1611 1001 901 262 1553 Fragments arranged by size: 1611 1553 1001 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4242 493 Size: 3749 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6451 SphI G_CATG'C Cuts at: 1132 2924 3809 1132 Size: 1792 885 3774 Fragments arranged by size: 3774 1792 885 SspI AAT'ATT Cuts at: 5 52 4375 5 Size: 47 4323 2081 Fragments arranged by size: 4323 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6451 StyI C'CwwG_G Cuts at: 513 1065 2004 2124 2197 2496 3890 513 Size: 552 939 120 73 299 1394 3074 Fragments arranged by size: 3074 1394 939 552 299 120 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2513 2816 4478 4495 4648 4833 5278 Size: 1255 933 303 1662 17 153 185 445 Cuts at: 5278 325 Size: 1498 Fragments arranged by size: 1662 1498 1255 933 445 303 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 3692 4697 Size: 274 80 33 51 1443 132 1583 1005 Cuts at: 4697 6409 96 Size: 1712 138 Fragments arranged by size: 1712 1583 1443 1005 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6411 6443 6411 Size: 32 6419 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6451 XbaI T'CTAG_A Cuts at: 2596 3787 2596 Size: 1191 5260 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 2473 Size: 6451 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6451 XmaI C'CCGG_G Cuts at: 1870 3776 1870 Size: 1906 4545 XmnI GAAnn'nnTTC Cuts at: 2283 3616 4580 2283 Size: 1333 964 4154 Fragments arranged by size: 4154 1333 964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4259 276 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI