(Linear) (Six-base) MAPSORT of: KIBB9441.seq Check: 2753 from: 1 to: 1746 KIBB9441.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AccI GT'mk_AC Cuts at: 0 1703 1746 Size: 1703 43 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 992 1107 1363 1427 1478 1746 Size: 67 925 115 256 64 51 268 Fragments arranged by size: 925 268 256 115 67 64 51 AleI CACnn'nnGTG Cuts at: 0 871 1486 1746 Size: 871 615 260 Fragments arranged by size: 871 615 260 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1039 1381 1746 Size: 1039 342 365 Fragments arranged by size: 1039 365 342 AlwNI CAG_nnn'CTG Cuts at: 0 1081 1716 1746 Size: 1081 635 30 Fragments arranged by size: 1081 635 30 ApaI G_GGCC'C Cuts at: 0 1131 1746 Size: 1131 615 ApoI r'AATT_y Cuts at: 0 259 1746 Size: 259 1487 AseI AT'TA_AT Cuts at: 0 1651 1746 Size: 1651 95 AvaI C'yCGr_G Cuts at: 0 410 1746 Size: 410 1336 BamHI G'GATC_C Cuts at: 0 1401 1746 Size: 1401 345 BanI G'GyrC_C Cuts at: 0 1456 1746 Size: 1456 290 BanII G_rGCy'C Cuts at: 0 761 804 946 1120 1131 1746 Size: 761 43 142 174 11 615 Fragments arranged by size: 761 615 174 142 43 11 BbeI G_GCGC'C Cuts at: 0 1460 1746 Size: 1460 286 BbsI GAAGACnn'nnnn_ Cuts at: 0 145 1042 1484 1746 Size: 145 897 442 262 Fragments arranged by size: 897 442 262 145 BbvCI CC'TCA_GC Cuts at: 0 1344 1746 Size: 1344 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 287 321 391 425 1746 Size: 287 34 70 34 1321 Fragments arranged by size: 1321 287 70 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1638 1746 Size: 1638 108 BglII A'GATC_T Cuts at: 0 1537 1746 Size: 1537 209 BlpI GC'TnA_GC Cuts at: 0 481 1087 1746 Size: 481 606 659 Fragments arranged by size: 659 606 481 Bme1580I G_kGCm'C Cuts at: 0 1131 1746 Size: 1131 615 BmtI G_CTAG'C Cuts at: 0 1285 1746 Size: 1285 461 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1329 1746 Size: 1329 417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 519 869 1168 1624 1746 Size: 493 26 350 299 456 122 Fragments arranged by size: 493 456 350 299 122 26 Bpu10I CC'TnA_GC Cuts at: 0 1082 1344 1354 1746 Size: 1082 262 10 392 Fragments arranged by size: 1082 392 262 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1570 1746 Size: 1570 176 BsaI GGTCTCn'nnnn_ Cuts at: 0 1061 1746 Size: 1061 685 BsaHI Gr'CG_yC Cuts at: 0 1457 1746 Size: 1457 289 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 397 812 1565 1746 Size: 397 415 753 181 Fragments arranged by size: 753 415 397 181 BseYI C'CCAG_C Cuts at: 0 19 398 427 997 1746 Size: 19 379 29 570 749 Fragments arranged by size: 749 570 379 29 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 968 1438 1746 Size: 968 470 308 Fragments arranged by size: 968 470 308 Bsp1286I G_dGCh'C Cuts at: 0 761 804 946 1120 1131 1746 Size: 761 43 142 174 11 615 Fragments arranged by size: 761 615 174 142 43 11 BspHI T'CATG_A Cuts at: 0 1695 1746 Size: 1695 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1701 1746 Size: 1701 45 BsrDI GCAATG_nn' Cuts at: 0 1253 1746 Size: 1253 493 BsrGI T'GTAC_A Cuts at: 0 104 1746 Size: 104 1642 BstXI CCAn_nnnn'nTGG Cuts at: 0 439 1746 Size: 439 1307 BstYI r'GATC_y Cuts at: 0 1401 1537 1746 Size: 1401 136 209 Fragments arranged by size: 1401 209 136 Bsu36I CC'TnA_GG Cuts at: 0 1424 1746 Size: 1424 322 BtgI C'CryG_G Cuts at: 0 192 1746 Size: 192 1554 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 60 1746 Size: 60 1686 BtsI GCAGTG_nn' Cuts at: 0 1037 1481 1493 1746 Size: 1037 444 12 253 Fragments arranged by size: 1037 444 253 12 EarI CTCTTCn'nnn_ Cuts at: 0 374 653 1746 Size: 374 279 1093 Fragments arranged by size: 1093 374 279 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 493 519 869 992 1107 1168 1363 Size: 67 426 26 350 123 115 61 195 Cuts at: 1363 1427 1478 1624 1746 Size: 64 51 146 122 Fragments arranged by size: 426 350 195 146 123 122 115 67 64 61 51 26 EcoO109I rG'GnC_Cy Cuts at: 0 155 1127 1128 1746 Size: 155 972 1 618 Fragments arranged by size: 972 618 155 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 197 1746 Size: 197 1549 HaeII r_GCGC'y Cuts at: 0 10 1460 1746 Size: 10 1450 286 Fragments arranged by size: 1450 286 10 HincII GTy'rAC Cuts at: 0 1199 1746 Size: 1199 547 HindIII A'AGCT_T Cuts at: 0 300 1746 Size: 300 1446 KasI G'GCGC_C Cuts at: 0 1456 1746 Size: 1456 290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 946 1746 Size: 946 800 MslI CAynn'nnrTG Cuts at: 0 871 1021 1486 1746 Size: 871 150 465 260 Fragments arranged by size: 871 465 260 150 MspA1I CmG'CkG Cuts at: 0 970 1153 1273 1746 Size: 970 183 120 473 Fragments arranged by size: 970 473 183 120 NarI GG'CG_CC Cuts at: 0 1457 1746 Size: 1457 289 NcoI C'CATG_G Cuts at: 0 192 1746 Size: 192 1554 NheI G'CTAG_C Cuts at: 0 1281 1746 Size: 1281 465 NspI r_CATG'y Cuts at: 0 919 1746 Size: 919 827 PasI CC'CwG_GG Cuts at: 0 541 819 1746 Size: 541 278 927 Fragments arranged by size: 927 541 278 PflMI CCAn_nnn'nTGG Cuts at: 0 131 1746 Size: 131 1615 PfoI T'CCnGG_A Cuts at: 0 470 1640 1746 Size: 470 1170 106 Fragments arranged by size: 1170 470 106 PpuMI rG'GwC_Cy Cuts at: 0 155 1746 Size: 155 1591 PshAI GACnn'nnGTC Cuts at: 0 1053 1746 Size: 1053 693 PspOMI G'GGCC_C Cuts at: 0 1127 1746 Size: 1127 619 PstI C_TGCA'G Cuts at: 0 448 1488 1746 Size: 448 1040 258 Fragments arranged by size: 1040 448 258 PvuII CAG'CTG Cuts at: 0 970 1153 1273 1746 Size: 970 183 120 473 Fragments arranged by size: 970 473 183 120 SfcI C'TryA_G Cuts at: 0 444 1484 1746 Size: 444 1040 262 Fragments arranged by size: 1040 444 262 SfoI GGC'GCC Cuts at: 0 1458 1746 Size: 1458 288 SmlI C'TyrA_G Cuts at: 0 1549 1746 Size: 1549 197 SphI G_CATG'C Cuts at: 0 919 1746 Size: 919 827 StyI C'CwwG_G Cuts at: 0 119 192 491 1746 Size: 119 73 299 1255 Fragments arranged by size: 1255 299 119 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 508 811 1746 Size: 508 303 935 Fragments arranged by size: 935 508 303 TatI w'GTAC_w Cuts at: 0 104 1687 1746 Size: 104 1583 59 Fragments arranged by size: 1583 104 59 XbaI T'CTAG_A Cuts at: 0 591 1746 Size: 591 1155 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 468 1746 Size: 468 1278 XmnI GAAnn'nnTTC Cuts at: 0 278 1611 1746 Size: 278 1333 135 Fragments arranged by size: 1333 278 135 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI Bsp1286I BspHI BspMI BsrDI BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI EarI Eco57MI EcoO109I FalI HaeII HincII HindIII KasI MmeI MslI MspA1I NarI NcoI NheI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SfcI SfoI SmlI SphI StyI TaqII TatI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AsiSI AvrII BaeI BclI BfrBI BglI BmgBI BmrI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XhoI XmaI ZraI