################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeIII HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeII MamI MfeI MflI MluNI MroI MscI MseI MslI MspA1I MunI MvaI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AcyI 6 GGCGTC G[GA]^CG[CT]C AflIII 83 ACGCGT A^C[GA][CT]GT AluI 52 AGCT AG^CT AlwNI 64 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 51 GAGCTC G[AT]GC[AT]^C AvaI 171 CTCGGG C^[CT]CG[GA]G AvaII 146 GGACC G^G[AT]CC BanII 51 GAGCTC G[GA]GC[CT]^C BcnI 110 CCGGG CC^[GC]GG BfaI 205 CTAG C^TAG BmyI 51 GAGCTC G[AGT]GC[ACT]^C BsaHI 6 GGCGTC G[GA]^CG[CT]C BseRI 136 CTCCTC GAGGAG(10/8) BsgI 310 CTGCAC GTGCAG(16/14) BsiHKAI 51 GAGCTC G[AT]GC[AT]^C BsmAI 134 GTCTC GTCTC(1/5) BsmFI 145 GGGAC GGGAC(10/14) BsoBI 171 CTCGGG C^[CT]CG[GA]G Bsp1286I 51 GAGCTC G[AGT]GC[ACT]^C BspHI 323 TCATGA T^CATGA BspMI 89 ACCTGC ACCTGC(4/8) BssKI 110 CCGGG ^CC[ACGT]GG BssSI 139 CTCGTG CACGAG(-5/-1) CfoI 115 GCGC GCG^C Cfr13I 146 GGACC G^G[ACGT]CC DpnII 228 GATC ^GATC DraII 145 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 40 GAAGAG CTCTTC(1/4) EcoO109I 145 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 255 CCAAGG C^C[AT][AT]GG HaeII 114 GGCGCT [GA]GCGC^[CT] HapII 110 CCGG C^CGG HhaI 115 GCGC GCG^C Hin1I 6 GGCGTC G[GA]^CG[CT]C HinP1I 115 GCGC G^CGC HpaII 110 CCGG C^CGG Ksp632I 40 GAAGAG CTCTTC(1/4) MaeI 205 CTAG C^TAG MaeIII 128 GTTAC ^GT[ACGT]AC MboI 228 GATC ^GATC MboII 40 GAAGA GAAGA(8/7) MluI 83 ACGCGT A^CGCGT MnlI 138 CCTC CCTC(7/6) MspI 110 CCGG C^CGG NciI 110 CCGGG CC^[GC]GG NdeII 228 GATC ^GATC NlaIII 324 CATG CATG^ NlaIV 145 GGGACC GG[ACGT]^[ACGT]CC PpuMI 145 GGGACCT [GA]G^G[AT]CC[CT] PstI 118 CTGCAG CTGCA^G RcaI 323 TCATGA T^CATGA SacI 51 GAGCTC GAGCT^C Sau3AI 228 GATC ^GATC Sau96I 146 GGACC G^G[ACGT]CC ScaI 44 AGTACT AGT^ACT ScrFI 110 CCGGG CC^[ACGT]GG SfaNI 260 GCATC GCATC(5/9) SfcI 118 CTGCAG C^T[GA][CT]AG SmlI 48 CTTGAG C^T[CT][GA]AG StyI 255 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 29 CGCG CG^CG 84 CGCG AfaI 45 GTAC GT^AC 87 GTAC BbvI 63 GCAGC GCAGC(8/12) 117 GCTGC BsaJI 110 CCGGGG C^C[ACGT][ACGT]GG 255 CCAAGG BstF5I 201 CATCC GGATG(2/0) 306 CATCC BstUI 29 CGCG CG^CG 84 CGCG Cac8I 273 GCGAGC GC[ACGT]^[ACGT]GC 298 GCCAGC DdeI 36 CTTAG C^T[ACGT]AG 217 CTGAG Fnu4HI 63 GCAGC GC^[ACGT]GC 117 GCTGC FokI 201 CATCC GGATG(9/13) 306 CATCC ItaI 63 GCAGC GC^[ACGT]GC 117 GCTGC MvnI 29 CGCG CG^CG 84 CGCG RsaI 45 GTAC GT^AC 87 GTAC TseI 63 GCAGC G^C[AT]GC 117 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### AciI HgaI