################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspHI AspI AvrII BamHI BanII BbeI BbrPI BbvCI BciVI BclI BfaI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FauI FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MaeII MamI MfeI MluI MroI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 251 GGTACC G^GTACC AflII 120 CTTAAG C^TTAAG AhdI 22 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 256 CCTCAGG CC^T[ACGT]AGG Asp718I 251 GGTACC G^GTACC AspEI 22 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 439 CTCGAG C^[CT]CG[GA]G AviII 89 TGCGCA TGC^GCA BbsI 550 GTCTTC GAAGAC(2/6) BcgI 126 CGATGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 333 CCGGG CC^[GC]GG BfrI 120 CTTAAG C^TTAAG BglI 403 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 550 GTCTTC GAAGAC(2/6) BsoBI 439 CTCGAG C^[CT]CG[GA]G BstBI 553 TTCGAA TT^CGAA BstYI 99 GGATCT [GA]^GATC[CT] Bsu36I 256 CCTCAGG CC^T[ACGT]AGG DsaI 530 CCGTGG C^C[GA][CT]GG Eam1105I 22 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 616 CTCTTC CTCTTC(1/4) Eco81I 256 CCTCAGG CC^T[ACGT]AGG EcoT14I 650 CCAAGG C^C[AT][AT]GG EcoT22I 523 ATGCAT ATGCA^T FspI 89 TGCGCA TGC^GCA HgaI 222 GACGC GACGC(5/10) HinfI 312 GACTC G^A[ACGT]TC KpnI 251 GGTACC GGTAC^C Ksp632I 616 CTCTTC CTCTTC(1/4) MaeIII 424 GTGAC ^GT[ACGT]AC MflI 99 GGATCT [GA]^GATC[CT] NciI 333 CCGGG CC^[GC]GG NsiI 523 ATGCAT ATGCA^T NspV 553 TTCGAA TT^CGAA PaeR7I 439 CTCGAG C^TCGAG PflMI 390 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 312 GACTC GAGTC(4/5) SbfI 264 CCTGCAGG CCTGCA^GG SfuI 553 TTCGAA TT^CGAA Sse8387I 264 CCTGCAGG CCTGCA^GG StuI 464 AGGCCT AGG^CCT StyI 650 CCAAGG C^C[AT][AT]GG Tsp45I 424 GTGAC ^GT[GC]AC Van91I 390 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 439 CTCGAG C^TCGAG XhoII 99 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 714 GATCC GGATC(4/5) 99 GGATC BanI 159 GGTGCC G^G[CT][GA]CC 251 GGTACC BmyI 134 GTGCCC G[AGT]GC[ACT]^C 275 GTGCCC BpmI 454 CTCCAG CTGGAG(16/14) 7 CTGGAG Bsp1286I 134 GTGCCC G[AGT]GC[ACT]^C 275 GTGCCC DdeI 257 CTCAG C^T[ACGT]AG 492 CTTAG DpnII 100 GATC ^GATC 714 GATC EcoNI 150 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 615 CCTCTTCCAGG HapII 333 CCGG C^CGG 717 CCGG HpaII 333 CCGG C^CGG 717 CCGG MboI 100 GATC ^GATC 714 GATC MseI 107 TTAA T^TAA 121 TTAA MspI 333 CCGG C^CGG 717 CCGG NdeII 100 GATC ^GATC 714 GATC Sau3AI 100 GATC ^GATC 714 GATC Tru9I 107 TTAA T^TAA 121 TTAA TspRI 395 CACTG CA[GC]TG[ACGT][ACGT]^ 602 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AvaII BalI BbvI BsaJI BsiYI BslI BsmFI BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MboII MluNI MnlI MscI MvaI MvnI MwoI NlaIII NlaIV PpuMI PstI RsaI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI TthHB8I