(Circular) (Six-base) MAPSORT of: pFN21AE6223.seq Check: 6519 from: 1 to: 5425 pFN21AE6223 5425 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2677 2677 Size: 5425 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2256 2746 2256 Size: 490 4935 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2669 3488 4872 5140 1632 Size: 1037 819 1384 268 1917 Fragments arranged by size: 1917 1384 1037 819 268 AflII C'TTAA_G Cuts at: 820 1017 2125 820 Size: 197 1108 4120 Fragments arranged by size: 4120 1108 197 AflIII A'CryG_T Cuts at: 1203 4324 5301 1203 Size: 3121 977 1327 Fragments arranged by size: 3121 1327 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 2032 4154 2032 Size: 2122 3303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2295 1184 Size: 1111 4314 AlwNI CAG_nnn'CTG Cuts at: 2269 2401 2695 3168 4740 2269 Size: 132 294 473 1572 2954 Fragments arranged by size: 2954 1572 473 294 132 ApaLI G'TGCA_C Cuts at: 3482 4638 3482 Size: 1156 4269 ApoI r'AATT_y Cuts at: 1493 2734 2960 3112 5221 1493 Size: 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2444 2750 1870 Size: 73 501 306 4545 Fragments arranged by size: 4545 501 306 73 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2164 2256 2746 4201 618 Size: 325 217 627 377 92 490 1455 1842 Fragments arranged by size: 1842 1455 627 490 377 325 217 92 BanII G_rGCy'C Cuts at: 1391 2744 1391 Size: 1353 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2548 928 Size: 971 649 3805 Fragments arranged by size: 3805 971 649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 3616 3650 1903 Size: 34 183 34 1462 34 3678 Fragments arranged by size: 3678 1462 183 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3317 4527 3317 Size: 1210 4215 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BfrBI ATG'CAT Cuts at: 2530 2530 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2414 4036 136 Size: 107 122 71 631 605 742 1622 1525 Fragments arranged by size: 1622 1525 742 631 605 122 107 71 BglII A'GATC_T Cuts at: 5420 5420 Size: 5425 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2143 2284 3486 4642 1790 Size: 353 141 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 353 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 4114 442 Size: 416 53 83 399 24 1739 958 1753 Fragments arranged by size: 1753 1739 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2444 4085 1239 Size: 794 411 1641 2579 Fragments arranged by size: 2579 1641 794 411 Bpu10I CC'TnA_GC Cuts at: 2497 2497 Size: 5425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 3550 4415 4713 2141 Size: 1409 865 298 2853 Fragments arranged by size: 2853 1409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4088 882 Size: 617 2589 2219 Fragments arranged by size: 2589 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3216 493 Size: 711 2012 2702 Fragments arranged by size: 2702 2012 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3232 3614 4249 275 Size: 53 83 186 936 1699 382 635 1451 Fragments arranged by size: 1699 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3858 4256 4530 4677 5088 3858 Size: 398 274 147 411 4195 Fragments arranged by size: 4195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5200 Fragments arranged by size: 5200 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5425 BseYI C'CCAG_C Cuts at: 2037 4628 5124 2037 Size: 2591 496 2338 Fragments arranged by size: 2591 2338 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4852 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2875 3636 4664 1540 Size: 275 186 874 761 1028 2301 Fragments arranged by size: 2301 1028 874 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 3571 4642 3486 Size: 85 1071 4269 Fragments arranged by size: 4269 1071 85 BsmI GAATG_Cn' Cuts at: 2936 3029 2936 Size: 93 5332 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2284 2744 3486 3571 4642 1391 Size: 399 353 141 460 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 353 141 85 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 3314 5044 3314 Size: 1730 3695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 2277 2677 2780 844 Size: 1193 240 400 103 3489 Fragments arranged by size: 3489 1193 400 240 103 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3920 4094 65 Size: 1183 2672 174 1396 Fragments arranged by size: 2672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4069 4256 1700 Size: 110 2259 187 2869 Fragments arranged by size: 2869 2259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5425 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5425 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5425 BstBI TT'CG_AA Cuts at: 2559 4229 2559 Size: 1670 3755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5425 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5425 BstYI r'GATC_y Cuts at: 1972 2104 2755 3122 3507 3524 4976 5062 5074 Size: 132 651 367 385 17 1452 86 12 Cuts at: 5074 5420 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 651 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2262 2262 Size: 5425 BtgI C'CryG_G Cuts at: 513 1065 2004 2535 513 Size: 552 939 531 3403 Fragments arranged by size: 3403 939 552 531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2145 487 Size: 683 56 919 3767 Fragments arranged by size: 3767 919 683 56 BtsI GCAGTG_nn' Cuts at: 2613 2941 3735 3755 2613 Size: 328 794 20 4283 Fragments arranged by size: 4283 794 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2577 2712 Size: 54 1425 50 275 18 378 369 135 Cuts at: 2712 2872 3761 5390 8 Size: 160 889 1629 43 Fragments arranged by size: 1629 1425 889 378 369 275 160 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2627 3355 1353 Size: 1274 728 3423 Fragments arranged by size: 3423 1274 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4012 4386 4532 387 Size: 1064 138 2423 374 146 1280 Fragments arranged by size: 2423 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2444 2669 3488 4085 4872 5140 Size: 393 401 411 225 819 597 787 268 Cuts at: 5140 1239 Size: 1524 Fragments arranged by size: 1524 819 787 597 411 401 393 268 225 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2624 2159 Size: 465 4960 EcoO109I rG'GnC_Cy Cuts at: 2242 2266 2617 2242 Size: 24 351 5050 Fragments arranged by size: 5050 351 24 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 2096 3931 2096 Size: 1835 3590 HaeII r_GCGC'y Cuts at: 1678 4572 1678 Size: 2894 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2101 2133 2372 Size: 32 135 32 446 32 51 32 239 Cuts at: 2372 2404 4066 4098 4140 4172 1373 Size: 32 1662 32 42 32 2626 Fragments arranged by size: 2626 1662 446 239 135 51 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2260 2750 2260 Size: 490 4935 MfeI C'AATT_G Cuts at: 3024 3024 Size: 5425 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2403 4539 4723 1305 Size: 1098 2136 184 2007 Fragments arranged by size: 2136 2007 1098 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2579 2714 10 Size: 54 1425 343 378 369 135 2721 Fragments arranged by size: 2721 1425 378 369 343 135 54 MslI CAynn'nnrTG Cuts at: 518 1166 3383 3742 3901 518 Size: 648 2217 359 159 2042 Fragments arranged by size: 2217 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2841 3518 4666 4911 1606 Size: 1235 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3934 Fragments arranged by size: 3934 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NsiI A_TGCA'T Cuts at: 2532 2532 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2783 4328 1132 Size: 97 1554 1545 2229 Fragments arranged by size: 2229 1554 1545 97 PasI CC'CwG_GG Cuts at: 1348 2703 1348 Size: 1355 4070 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 1108 Size: 1293 4132 PfoI T'CCnGG_A Cuts at: 2625 2625 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2470 3494 3526 5007 5039 2438 Size: 32 1024 32 1481 32 2824 Fragments arranged by size: 2824 1481 1024 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2266 2617 2242 Size: 24 351 5050 Fragments arranged by size: 5050 351 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5425 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PspXI vC'TCGA_Gb Cuts at: 2444 2444 Size: 5425 PstI C_TGCA'G Cuts at: 830 2274 2439 2674 2777 830 Size: 1444 165 235 103 3478 Fragments arranged by size: 3478 1444 235 165 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SbfI CC_TGCA'GG Cuts at: 2274 2777 2274 Size: 503 4922 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SfcI C'TryA_G Cuts at: 826 1045 2270 2435 2670 2773 3908 4589 4780 Size: 219 1225 165 235 103 1135 681 191 Cuts at: 4780 826 Size: 1471 Fragments arranged by size: 1471 1225 1135 681 235 219 191 165 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2752 1872 Size: 880 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2125 2156 2444 3529 4430 4692 Size: 197 926 182 31 288 1085 901 262 Cuts at: 4692 820 Size: 1553 Fragments arranged by size: 1553 1085 926 901 288 262 197 182 31 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2783 1132 Size: 1651 3774 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 2471 1517 Size: 954 4471 StyI C'CwwG_G Cuts at: 513 1065 2004 2655 2864 513 Size: 552 939 651 209 3074 Fragments arranged by size: 3074 939 651 552 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2366 2528 2695 3452 3469 3622 3807 Size: 1255 786 162 167 757 17 153 185 Cuts at: 3807 4252 325 Size: 445 1498 Fragments arranged by size: 1498 1255 786 757 445 185 167 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3671 5383 96 Size: 274 80 33 51 1443 1694 1712 138 Fragments arranged by size: 1712 1694 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5385 5417 5385 Size: 32 5393 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5425 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 2444 1943 Size: 501 4924 XmaI C'CCGG_G Cuts at: 1870 2750 1870 Size: 880 4545 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI