(Linear) (Six-base) MAPSORT of: KIEE6223.seq Check: 1714 from: 1 to: 720 KIEE6223.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 672 720 Size: 672 48 Acc65I G'GTAC_C Cuts at: 0 251 720 Size: 251 469 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 664 720 Size: 664 56 AflII C'TTAA_G Cuts at: 0 120 720 Size: 120 600 AhdI GACnn_n'nnGTC Cuts at: 0 27 720 Size: 27 693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 290 720 Size: 290 430 AlwNI CAG_nnn'CTG Cuts at: 0 264 396 690 720 Size: 264 132 294 30 Fragments arranged by size: 294 264 132 30 AvaI C'yCGr_G Cuts at: 0 439 720 Size: 439 281 BanI G'GyrC_C Cuts at: 0 159 251 720 Size: 159 92 469 Fragments arranged by size: 469 159 92 BbsI GAAGACnn'nnnn_ Cuts at: 0 543 720 Size: 543 177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 115 149 720 Size: 115 34 571 Fragments arranged by size: 571 115 34 BfrBI ATG'CAT Cuts at: 0 525 720 Size: 525 195 BglI GCCn_nnn'nGGC Cuts at: 0 409 720 Size: 409 311 Bme1580I G_kGCm'C Cuts at: 0 138 279 720 Size: 138 141 441 Fragments arranged by size: 441 141 138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 439 720 Size: 28 411 281 Fragments arranged by size: 411 281 28 Bpu10I CC'TnA_GC Cuts at: 0 492 720 Size: 492 228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 720 Size: 136 584 BseYI C'CCAG_C Cuts at: 0 32 720 Size: 32 688 Bsp1286I G_dGCh'C Cuts at: 0 138 279 720 Size: 138 141 441 Fragments arranged by size: 441 141 138 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 32 272 672 720 Size: 32 240 400 48 Fragments arranged by size: 400 240 48 32 BstBI TT'CG_AA Cuts at: 0 554 720 Size: 554 166 BstYI r'GATC_y Cuts at: 0 99 720 Size: 99 621 Bsu36I CC'TnA_GG Cuts at: 0 257 720 Size: 257 463 BtgI C'CryG_G Cuts at: 0 530 720 Size: 530 190 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 140 720 Size: 140 580 BtsI GCAGTG_nn' Cuts at: 0 608 720 Size: 608 112 EaeI y'GGCC_r Cuts at: 0 203 572 707 720 Size: 203 369 135 13 Fragments arranged by size: 369 203 135 13 EarI CTCTTCn'nnn_ Cuts at: 0 622 720 Size: 622 98 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 439 664 720 Size: 28 411 225 56 Fragments arranged by size: 411 225 56 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 154 619 720 Size: 154 465 101 Fragments arranged by size: 465 154 101 EcoO109I rG'GnC_Cy Cuts at: 0 237 261 612 720 Size: 237 24 351 108 Fragments arranged by size: 351 237 108 24 FspI TGC'GCA Cuts at: 0 91 720 Size: 91 629 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 96 128 367 399 720 Size: 13 32 51 32 239 32 321 Fragments arranged by size: 321 239 51 32 32 32 13 KpnI G_GTAC'C Cuts at: 0 255 720 Size: 255 465 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 398 720 Size: 398 322 MscI TGG'CCA Cuts at: 0 205 574 709 720 Size: 205 369 135 11 Fragments arranged by size: 369 205 135 11 NsiI A_TGCA'T Cuts at: 0 527 720 Size: 527 193 PasI CC'CwG_GG Cuts at: 0 698 720 Size: 698 22 PflMI CCAn_nnn'nTGG Cuts at: 0 396 720 Size: 396 324 PfoI T'CCnGG_A Cuts at: 0 620 720 Size: 620 100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 433 465 720 Size: 433 32 255 Fragments arranged by size: 433 255 32 PpuMI rG'GwC_Cy Cuts at: 0 237 261 612 720 Size: 237 24 351 108 Fragments arranged by size: 351 237 108 24 PspXI vC'TCGA_Gb Cuts at: 0 439 720 Size: 439 281 PstI C_TGCA'G Cuts at: 0 269 434 669 720 Size: 269 165 235 51 Fragments arranged by size: 269 235 165 51 SbfI CC_TGCA'GG Cuts at: 0 269 720 Size: 269 451 SfcI C'TryA_G Cuts at: 0 265 430 665 720 Size: 265 165 235 55 Fragments arranged by size: 265 235 165 55 SmlI C'TyrA_G Cuts at: 0 120 151 439 720 Size: 120 31 288 281 Fragments arranged by size: 288 281 120 31 StuI AGG'CCT Cuts at: 0 466 720 Size: 466 254 StyI C'CwwG_G Cuts at: 0 650 720 Size: 650 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 361 523 690 720 Size: 361 162 167 30 Fragments arranged by size: 361 167 162 30 XhoI C'TCGA_G Cuts at: 0 439 720 Size: 439 281 Enzymes that do cut: AarI Acc65I AcuI AflII AhdI AlfI AlwNI AvaI BanI BbsI BcgI BfrBI BglI Bme1580I BpmI Bpu10I BpuEI BseYI Bsp1286I BspMI BstBI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FspI Hin4I KpnI MmeI MscI NsiI PasI PflMI PfoI PpiI PpuMI PspXI PstI SbfI SfcI SmlI StuI StyI TaqII XhoI Enzymes that do not cut: AatII AccI AclI AfeI AflIII AgeI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI