################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## Acc65I AccI AccII AccIII AciI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 37 GACGTC GACGT^C AfaI 261 GTAC GT^AC AlwNI 76 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 94 GAGCTC G[AT]GC[AT]^C AvaII 177 GGACC G^G[AT]CC BanII 94 GAGCTC G[GA]GC[CT]^C BclI 29 TGATCA T^GATCA BfaI 10 CTAG C^TAG BmyI 94 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 94 GAGCTC G[AT]GC[AT]^C BsmAI 13 GAGAC GTCTC(1/5) Bsp1286I 94 GAGCTC G[AGT]GC[ACT]^C BstF5I 153 GGATG GGATG(2/0) Cac8I 71 GCACGC GC[ACGT]^[ACGT]GC Cfr13I 177 GGACC G^G[ACGT]CC DpnII 30 GATC ^GATC DraII 176 AGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 81 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 176 AGGACCT [GA]G^G[ACGT]CC[CT] FbaI 29 TGATCA T^GATCA FokI 153 GGATG GGATG(9/13) HgaI 184 GACGC GACGC(5/10) HinfI 278 GACTC G^A[ACGT]TC MaeI 10 CTAG C^TAG MaeIII 254 GTCAC ^GT[ACGT]AC MboI 30 GATC ^GATC MnlI 64 GAGG CCTC(7/6) MslI 21 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 20 CCATGG C^CATGG NdeII 30 GATC ^GATC PleI 278 GACTC GAGTC(4/5) PpuMI 176 AGGACCT [GA]G^G[AT]CC[CT] PstI 133 CTGCAG CTGCA^G RsaI 261 GTAC GT^AC SacI 94 GAGCTC GAGCT^C Sau3AI 30 GATC ^GATC Sau96I 177 GGACC G^G[ACGT]CC ScaI 260 AGTACT AGT^ACT SfcI 133 CTGCAG C^T[GA][CT]AG Tsp45I 254 GTCAC ^GT[GC]AC Tsp509I 167 AATT ^AATT TspRI 213 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcyI 37 GACGTC G[GA]^CG[CT]C 184 GACGCC AluI 95 AGCT AG^CT 119 AGCT BsaHI 37 GACGTC G[GA]^CG[CT]C 184 GACGCC BsiYI 48 CCGATATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 81 CCTGACCAAGG BslI 48 CCGATATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 81 CCTGACCAAGG DsaI 20 CCATGG C^C[GA][CT]GG 158 CCGTGG EcoT14I 20 CCATGG C^C[AT][AT]GG 86 CCAAGG Hin1I 37 GACGTC G[GA]^CG[CT]C 184 GACGCC MaeII 38 ACGT A^CGT 244 ACGT NlaIII 21 CATG CATG^ 27 CATG SfaNI 154 GATGC GCATC(5/9) 196 GATGC SmlI 97 CTCAAG C^T[CT][GA]AG 172 CTCAAG StyI 20 CCATGG C^C[AT][AT]GG 86 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BbvI BsaJI BssKI BstNI EcoRII Fnu4HI ItaI MvaI MwoI ScrFI TseI