(Linear) (Six-base) MAPSORT of: KIBB8859.seq  Check: 293  from: 1  to: 285



KIBB8859.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:32  ..

AatII G_ACGT'C

Cuts at:      0      41     285 
   Size:         41     244
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     285 
   Size:        195      90
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     285 
   Size:         81     204
 
BanII G_rGCy'C

Cuts at:      0      98     285 
   Size:         98     187
 
BclI T'GATC_A

Cuts at:      0      29     285 
   Size:         29     256
 
BmgBI CAC'GTC

Cuts at:      0     245     285 
   Size:        245      40
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     157     285 
   Size:         82      75     128

  Fragments arranged by size: 

                 128      82      75
 
BsaHI Gr'CG_yC

Cuts at:      0      38     185     285 
   Size:         38     147     100

  Fragments arranged by size: 

                 147     100      38
 
BsiHKAI G_wGCw'C

Cuts at:      0      98     285 
   Size:         98     187
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     285 
   Size:         98     187
 
BtgI C'CryG_G

Cuts at:      0      20     158     285 
   Size:         20     138     127

  Fragments arranged by size: 

                 138     127      20
 
EcoICRI GAG'CTC

Cuts at:      0      96     285 
   Size:         96     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     285 
   Size:        195      90
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      85     285 
   Size:         85     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0     177     285 
   Size:        177     108
 
MslI CAynn'nnrTG

Cuts at:      0      25     285 
   Size:         25     260
 
NcoI C'CATG_G

Cuts at:      0      20     285 
   Size:         20     265
 
PpuMI rG'GwC_Cy

Cuts at:      0     177     285 
   Size:        177     108
 
PstI C_TGCA'G

Cuts at:      0     137     285 
   Size:        137     148
 
SacI G_AGCT'C

Cuts at:      0      98     285 
   Size:         98     187
 
ScaI AGT'ACT

Cuts at:      0     262     285 
   Size:        262      23
 
SfcI C'TryA_G

Cuts at:      0     133     285 
   Size:        133     152
 
SmlI C'TyrA_G

Cuts at:      0      97     172     285 
   Size:         97      75     113

  Fragments arranged by size: 

                 113      97      75
 
StyI C'CwwG_G

Cuts at:      0      20      86     285 
   Size:         20      66     199

  Fragments arranged by size: 

                 199      66      20
 
TatI w'GTAC_w

Cuts at:      0     260     285 
   Size:        260      25
 
ZraI GAC'GTC

Cuts at:      0      39     285 
   Size:         39     246
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI    BanII     BclI    BmgBI    BpuEI    BsaHI
  BsiHKAI Bsp1286I     BtgI  EcoICRI  Eco57MI    EcoNI EcoO109I     MslI
     NcoI    PpuMI     PstI     SacI     ScaI     SfcI     SmlI     StyI
     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI