################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 79 GTCGAC GT^[AC][GT]AC AcsI 30 GAATTC [GA]^AATT[CT] ApoI 30 GAATTC [GA]^AATT[CT] AspI 360 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 603 GGATCC G^GATCC BcnI 98 CCCGG CC^[GC]GG BfaI 308 CTAG C^TAG BlpI 151 GCTGAGC GC^T[ACGT]AGC Bpu1102I 151 GCTGAGC GC^T[ACGT]AGC BsaI 303 GGTCTC GGTCTC(1/5) BsgI 298 GTGCAG GTGCAG(16/14) BsmFI 514 GGGAC GGGAC(10/14) BspMI 300 GCAGGT ACCTGC(4/8) BsrFI 10 GCCGGC [GA]^CCGG[CT] BsrI 363 CCAGT ACTGG(1/-1) BstAPI 291 GCATGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 69 GGATG GGATG(2/0) BstYI 603 GGATCC [GA]^GATC[CT] CelII 151 GCTGAGC GC^T[ACGT]AGC Cfr10I 10 GCCGGC [GA]^CCGG[CT] DpnII 604 GATC ^GATC DraII 194 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 12 CGGCCA [CT]^GGCC[GA] EarI 169 GAAGAG CTCTTC(1/4) EcoNI 37 CCTAACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 194 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 30 GAATTC G^AATTC EcoRV 19 GATATC GAT^ATC FokI 69 GGATG GGATG(9/13) HaeII 310 AGCGCC [GA]GCGC^[CT] HincII 79 GTCGAC GT[CT]^[GA]AC HindII 79 GTCGAC GT[CT]^[GA]AC HindIII 460 AAGCTT A^AGCTT Ksp632I 169 GAAGAG CTCTTC(1/4) MaeI 308 CTAG C^TAG MaeII 296 ACGT A^CGT MboI 604 GATC ^GATC MflI 603 GGATCC [GA]^GATC[CT] MslI 84 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 291 GCATGACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 10 GCCGGC GCC^GGC NciI 98 CCCGG CC^[GC]GG NdeII 604 GATC ^GATC NgoMIV 10 GCCGGC G^CCGGC PflFI 360 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 194 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 422 CAGCTG CAG^CTG SalI 79 GTCGAC G^TCGAC Sau3AI 604 GATC ^GATC SmlI 229 CTCAAG C^T[CT][GA]AG TaqI 80 TCGA T^CGA Tth111I 360 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 80 TCGA T^CGA XhoII 603 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 604 GATCC GGATC(4/5) 603 GGATC AspHI 142 GAGCTC G[AT]GC[AT]^C 226 GAGCTC BbsI 381 GAAGAC GAAGAC(2/6) 414 GAAGAC BbvCI 572 CCTCAGC CCTCAGC(-5/-2) 130 GCTGAGG BpuAI 381 GAAGAC GAAGAC(2/6) 414 GAAGAC BsiHKAI 142 GAGCTC G[AT]GC[AT]^C 226 GAGCTC BsmAI 467 GAGAC GTCTC(1/5) 304 GTCTC Cac8I 10 GCCGGC GC[ACGT]^[ACGT]GC 287 GCTGGC CfoI 189 GCGC GCG^C 311 GCGC EcoT14I 161 CCAAGG C^C[AT][AT]GG 206 CCAAGG FauI 607 CCCGC CCCGC(4/6) 577 GCGGG HaeIII 13 GGCC GG^CC 244 GGCC HapII 11 CCGG C^CGG 99 CCGG HhaI 189 GCGC GCG^C 311 GCGC HinP1I 189 GCGC G^CGC 311 GCGC HpaII 11 CCGG C^CGG 99 CCGG HphI 538 GGTGA GGTGA(8/7) 586 GGTGA MspA1I 422 CAGCTG C[AC]G^C[GT]G 575 CAGCGG MspI 11 CCGG C^CGG 99 CCGG NlaIV 514 GGGACC GG[ACGT]^[ACGT]CC 603 GGATCC PflMI 192 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 281 CCAGGAGCTGG SacI 142 GAGCTC GAGCT^C 226 GAGCTC StyI 161 CCAAGG C^C[AT][AT]GG 206 CCAAGG Tsp509I 31 AATT ^AATT 177 AATT Van91I 192 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 281 CCAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI AlwNI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI DrdI EcoRII Fnu4HI HinfI ItaI MaeIII MboII MnlI MvaI NlaIII PleI Sau96I ScrFI TseI Tsp45I TspRI