(Circular) (Six-base) MAPSORT of: pFN21AE2584.seq Check: 7019 from: 1 to: 5323 pFN21AE2584 5323 bp With 182 enzymes: SgfI * August 7, 2015 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3133 278 Size: 53 83 186 936 1597 2468 Fragments arranged by size: 2468 1597 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2085 2666 1937 Size: 148 581 4594 Fragments arranged by size: 4594 581 148 Acc65I G'GTAC_C Cuts at: 2644 2644 Size: 5323 AclI AA'CG_TT Cuts at: 1563 3450 3823 1563 Size: 1887 373 3063 Fragments arranged by size: 3063 1887 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2409 3386 4770 5038 1632 Size: 777 977 1384 268 1917 Fragments arranged by size: 1917 1384 977 777 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5126 AflIII A'CryG_T Cuts at: 1203 4222 5199 1203 Size: 3019 977 1327 Fragments arranged by size: 3019 1327 977 AgeI A'CCGG_T Cuts at: 4154 4154 Size: 5323 AhdI GACnn_n'nnGTC Cuts at: 4052 4052 Size: 5323 AleI CACnn'nnGTG Cuts at: 1166 2093 1166 Size: 927 4396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5323 AlwNI CAG_nnn'CTG Cuts at: 2135 2203 2281 2292 3066 4638 2135 Size: 68 78 11 774 1572 2820 Fragments arranged by size: 2820 1572 774 78 68 11 ApaLI G'TGCA_C Cuts at: 3380 4536 3380 Size: 1156 4167 ApoI r'AATT_y Cuts at: 1493 2035 2632 2858 3010 5119 1493 Size: 542 597 226 152 2109 1697 Fragments arranged by size: 2109 1697 597 542 226 152 AseI AT'TA_AT Cuts at: 160 1032 3877 160 Size: 872 2845 1606 Fragments arranged by size: 2845 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5323 AvaI C'yCGr_G Cuts at: 1870 1943 2648 1870 Size: 73 705 4545 Fragments arranged by size: 4545 705 73 BamHI G'GATC_C Cuts at: 2608 2653 3020 2608 Size: 45 367 4911 Fragments arranged by size: 4911 367 45 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2644 4099 618 Size: 325 217 627 857 1455 1842 Fragments arranged by size: 1842 1455 857 627 325 217 BanII G_rGCy'C Cuts at: 1391 2151 2235 2450 2642 1391 Size: 760 84 215 192 4072 Fragments arranged by size: 4072 760 215 192 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2393 2426 928 Size: 971 494 33 3825 Fragments arranged by size: 3825 971 494 33 BbvCI CC'TCA_GC Cuts at: 2136 2578 2136 Size: 442 4881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3514 3548 1903 Size: 34 1577 34 3678 Fragments arranged by size: 3678 1577 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3215 4425 3215 Size: 1210 4113 BclI T'GATC_A Cuts at: 1548 1548 Size: 5323 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3934 136 Size: 107 122 71 631 605 2262 1525 Fragments arranged by size: 2262 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5318 5318 Size: 5323 BlpI GC'TnA_GC Cuts at: 2157 2740 2157 Size: 583 4740 Bme1580I G_kGCm'C Cuts at: 1790 3384 4540 1790 Size: 1594 1156 2573 Fragments arranged by size: 2573 1594 1156 BmgBI CAC'GTC Cuts at: 1318 2302 1318 Size: 984 4339 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2362 3054 4012 Size: 416 53 83 399 24 945 692 958 Cuts at: 4012 442 Size: 1753 Fragments arranged by size: 1753 958 945 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5323 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3983 1239 Size: 2744 2579 Bpu10I CC'TnA_GC Cuts at: 2136 2150 2578 2136 Size: 14 428 4881 Fragments arranged by size: 4881 428 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 3448 4313 4611 2219 Size: 1229 865 298 2931 Fragments arranged by size: 2931 1229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2314 3986 882 Size: 617 815 1672 2219 Fragments arranged by size: 2219 1672 815 617 BsaAI yAC'GTr Cuts at: 493 1204 3114 493 Size: 711 1910 2702 Fragments arranged by size: 2702 1910 711 BsaBI GATnn'nnATC Cuts at: 5317 5317 Size: 5323 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3130 3512 4147 275 Size: 53 83 186 936 1597 382 635 1451 Fragments arranged by size: 1597 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3756 4154 4428 4575 4986 3756 Size: 398 274 147 411 4093 Fragments arranged by size: 4093 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5098 Fragments arranged by size: 5098 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2156 2159 2254 1186 Size: 967 3 3 95 4255 Fragments arranged by size: 4255 967 95 3 3 BseYI C'CCAG_C Cuts at: 2245 4526 5022 2245 Size: 2281 496 2546 Fragments arranged by size: 2546 2281 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 1163 Size: 573 588 4162 Fragments arranged by size: 4162 588 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2773 3534 4562 1540 Size: 275 186 772 761 1028 2301 Fragments arranged by size: 2301 1028 772 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2235 3384 3469 4540 2151 Size: 84 1149 85 1071 2934 Fragments arranged by size: 2934 1149 1071 85 84 BsmI GAATG_Cn' Cuts at: 2834 2927 2834 Size: 93 5230 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2235 2450 2642 3384 3469 4540 Size: 399 361 84 215 192 742 85 1071 Cuts at: 4540 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 361 215 192 85 84 BspEI T'CCGG_A Cuts at: 4986 4986 Size: 5323 BspHI T'CATG_A Cuts at: 3212 4942 3212 Size: 1730 3593 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2296 2678 844 Size: 1452 382 3489 Fragments arranged by size: 3489 1452 382 BsrBI CCG'CTC Cuts at: 1394 1823 3210 1394 Size: 429 1387 3507 Fragments arranged by size: 3507 1387 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3818 3992 65 Size: 1183 2570 174 1396 Fragments arranged by size: 2570 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 3967 4154 1700 Size: 110 205 1952 187 2869 Fragments arranged by size: 2869 1952 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5323 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5323 BssSI C'ACGA_G Cuts at: 3383 4395 3383 Size: 1012 4311 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 794 Size: 1508 3815 BstBI TT'CG_AA Cuts at: 4127 4127 Size: 5323 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5323 BstYI r'GATC_y Cuts at: 1972 2608 2653 3020 3405 3422 4874 4960 4972 Size: 636 45 367 385 17 1452 86 12 Cuts at: 4972 5318 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 636 385 367 346 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3832 Fragments arranged by size: 3832 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4584 Fragments arranged by size: 4584 683 56 BtsI GCAGTG_nn' Cuts at: 2566 2839 3633 3653 2566 Size: 273 794 20 4236 Fragments arranged by size: 4236 794 273 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5323 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5288 DraI TTT'AAA Cuts at: 2627 2627 Size: 5323 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2306 2497 2527 4330 809 Size: 507 990 191 30 1803 1802 Fragments arranged by size: 1803 1802 990 507 191 30 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2770 3659 Size: 54 1425 50 275 18 187 753 889 Cuts at: 3659 5288 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 753 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2770 1537 Size: 275 958 4090 Fragments arranged by size: 4090 958 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 3253 1353 Size: 816 1084 3423 Fragments arranged by size: 3423 1084 816 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2002 3910 4284 4430 387 Size: 1064 138 413 1908 374 146 1280 Fragments arranged by size: 1908 1280 1064 413 374 146 138 EcoICRI GAG'CTC Cuts at: 2149 2233 2149 Size: 84 5239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 3386 3983 4770 5038 1239 Size: 393 777 977 597 787 268 1524 Fragments arranged by size: 1524 977 787 777 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2046 2046 Size: 5323 EcoO109I rG'GnC_Cy Cuts at: 2200 2200 Size: 5323 EcoRI G'AATT_C Cuts at: 2035 2632 3010 2035 Size: 597 378 4348 Fragments arranged by size: 4348 597 378 EcoRV GAT'ATC Cuts at: 2026 2026 Size: 5323 FspI TGC'GCA Cuts at: 3829 3829 Size: 5323 HaeII r_GCGC'y Cuts at: 1678 2319 4470 1678 Size: 641 2151 2531 Fragments arranged by size: 2531 2151 641 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3964 3996 4038 4070 1373 Size: 32 135 32 2392 32 42 32 2626 Fragments arranged by size: 2626 2392 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2086 2667 2913 669 Size: 988 281 148 581 246 3079 Fragments arranged by size: 3079 988 581 281 246 148 HindIII A'AGCT_T Cuts at: 748 2465 748 Size: 1717 3606 HpaI GTT'AAC Cuts at: 2913 2913 Size: 5323 KpnI G_GTAC'C Cuts at: 2648 2648 Size: 5323 MfeI C'AATT_G Cuts at: 2922 2922 Size: 5323 MluI A'CGCG_T Cuts at: 5199 5199 Size: 5323 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 4437 4621 1305 Size: 1281 1851 184 2007 Fragments arranged by size: 2007 1851 1281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3501 Fragments arranged by size: 3501 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 3281 3640 3799 518 Size: 648 927 1188 359 159 2042 Fragments arranged by size: 2042 1188 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2429 2582 2739 3416 4564 4809 1606 Size: 823 153 157 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 677 245 157 153 NaeI GCC'GGC Cuts at: 2017 2017 Size: 5323 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3832 Fragments arranged by size: 3832 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5323 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 5323 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5323 NotI GC'GGCC_GC Cuts at: 2770 2770 Size: 5323 NspI r_CATG'y Cuts at: 1132 1229 2681 4226 1132 Size: 97 1452 1545 2229 Fragments arranged by size: 2229 1545 1452 97 PasI CC'CwG_GG Cuts at: 1348 2260 1348 Size: 912 4411 PciI A'CATG_T Cuts at: 4222 4222 Size: 5323 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 2292 1108 Size: 1095 89 4139 Fragments arranged by size: 4139 1095 89 PfoI T'CCnGG_A Cuts at: 2285 2285 Size: 5323 PmeI GTTT'AAAC Cuts at: 2627 2627 Size: 5323 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3392 3424 4905 4937 3392 Size: 32 1481 32 3778 Fragments arranged by size: 3778 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2200 Size: 5323 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5323 PsiI TTA'TAA Cuts at: 2893 2893 Size: 5323 PstI C_TGCA'G Cuts at: 830 2675 830 Size: 1845 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5323 PvuII CAG'CTG Cuts at: 2429 2429 Size: 5323 SacI G_AGCT'C Cuts at: 2151 2235 2151 Size: 84 5239 SalI G'TCGA_C Cuts at: 1936 2084 2665 1936 Size: 148 581 4594 Fragments arranged by size: 4594 581 148 SbfI CC_TGCA'GG Cuts at: 2675 2675 Size: 5323 ScaI AGT'ACT Cuts at: 3571 5283 3571 Size: 1712 3611 SfcI C'TryA_G Cuts at: 826 1045 2671 3806 4487 4678 826 Size: 219 1626 1135 681 191 1471 Fragments arranged by size: 1626 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5323 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5323 SmaI CCC'GGG Cuts at: 1872 2650 1872 Size: 778 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 3427 4328 4590 820 Size: 197 926 291 1193 901 262 1553 Fragments arranged by size: 1553 1193 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 3114 493 Size: 2621 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5323 SphI G_CATG'C Cuts at: 1132 2681 1132 Size: 1549 3774 SspI AAT'ATT Cuts at: 5 52 3247 5 Size: 47 3195 2081 Fragments arranged by size: 3195 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5323 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 2211 2762 513 Size: 552 939 162 45 551 3074 Fragments arranged by size: 3074 939 552 551 162 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2526 2531 3350 3367 3520 3705 4150 Size: 1255 946 5 819 17 153 185 445 Cuts at: 4150 325 Size: 1498 Fragments arranged by size: 1498 1255 946 819 445 185 153 17 5 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3569 5281 96 Size: 274 80 33 51 1443 1592 1712 138 Fragments arranged by size: 1712 1592 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5283 5315 5283 Size: 32 5291 Tth111I GACn'n_nGTC Cuts at: 1316 2368 1316 Size: 1052 4271 XbaI T'CTAG_A Cuts at: 2659 2659 Size: 5323 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5323 XmaI C'CCGG_G Cuts at: 1870 2648 1870 Size: 778 4545 XmnI GAAnn'nnTTC Cuts at: 3452 3452 Size: 5323 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3131 276 Size: 53 83 186 936 1597 2468 Fragments arranged by size: 2468 1597 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI