################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 412 GACGTC GACGT^C AflIII 653 ACGCGT A^C[GA][CT]GT ApaI 8 GGGCCC GGGCC^C BpmI 425 CTCCAG CTGGAG(16/14) BsaAI 559 TACGTG [CT]AC^GT[GA] BsaI 201 GGTCTC GGTCTC(1/5) BseRI 603 GAGGAG GAGGAG(10/8) BsgI 172 CTGCAC GTGCAG(16/14) BsrDI 691 GCAATG GCAATG(2/0) BstEII 165 GGTGACC G^GT[ACGT]ACC BstPI 165 GGTGACC G^GT[ACGT]ACC BstXI 740 CCAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 133 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 144 CCATGG C^C[GA][CT]GG EcoO65I 165 GGTGACC G^GT[ACGT]ACC FauI 665 GCGGG CCCGC(4/6) HaeII 227 GGCGCT [GA]GCGC^[CT] HinfI 754 GAGTC G^A[ACGT]TC MluI 653 ACGCGT A^CGCGT NcoI 144 CCATGG C^CATGG NlaIII 145 CATG CATG^ PflMI 191 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 754 GAGTC GAGTC(4/5) PstI 598 CTGCAG CTGCA^G SapI 81 GCTCTTC GCTCTTC(1/4) SmaI 161 CCCGGG CCC^GGG Tsp509I 632 AATT ^AATT Van91I 191 CCAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 59 CCAGCCCTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 161 CCCGGG C^CCGGG XmaI 161 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 654 CGCG CG^CG 664 CGCG AcyI 412 GACGTC G[GA]^CG[CT]C 675 GACGCC AfaI 489 GTAC GT^AC 570 GTAC AvaI 161 CCCGGG C^[CT]CG[GA]G 526 CTCGGG BfaI 379 CTAG C^TAG 485 CTAG BsaHI 412 GACGTC G[GA]^CG[CT]C 675 GACGCC BsmAI 202 GTCTC GTCTC(1/5) 756 GTCTC BsoBI 161 CCCGGG C^[CT]CG[GA]G 526 CTCGGG BstUI 654 CGCG CG^CG 664 CGCG DpnII 264 GATC ^GATC 513 GATC EarI 82 CTCTTC CTCTTC(1/4) 87 CTCTTC Hin1I 412 GACGTC G[GA]^CG[CT]C 675 GACGCC HphI 165 GGTGA GGTGA(8/7) 221 TCACC Ksp632I 82 CTCTTC CTCTTC(1/4) 87 CTCTTC MaeI 379 CTAG C^TAG 485 CTAG MboI 264 GATC ^GATC 513 GATC MvnI 654 CGCG CG^CG 664 CGCG NdeII 264 GATC ^GATC 513 GATC PpuMI 307 GGGACCT [GA]G^G[AT]CC[CT] 338 GGGACCC RsaI 489 GTAC GT^AC 570 GTAC Sau3AI 264 GATC ^GATC 513 GATC SfaNI 253 GATGC GCATC(5/9) 323 GCATC SfcI 384 CTACAG C^T[GA][CT]AG 598 CTGCAG TaqI 262 TCGA T^CGA 658 TCGA TthHB8I 262 TCGA T^CGA 658 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI StyI TseI Tsp45I TspRI