################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BalI BanI BbeI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaI BseRI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MluI MluNI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PaeR7I PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1433 GTCTAC GT^[AC][GT]AC AccII 21 CGCG CG^CG AccIII 899 TCCGGA T^CCGGA AciI 22 GCGG CCGC(-3/-1) AcyI 919 GACGCC G[GA]^CG[CT]C AflII 75 CTTAAG C^TTAAG AflIII 26 ACACGT A^C[GA][CT]GT AlwNI 707 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 187 ATTAAT AT^TAAT AsnI 187 ATTAAT AT^TAAT Asp700I 1501 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 30 GTGCTC G[AT]GC[AT]^C AspI 1243 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 418 GGATCC G^GATCC BanII 1022 GAGCCC G[GA]GC[CT]^C BbrPI 27 CACGTG CAC^GTG BcnI 813 CCGGG CC^[GC]GG BfrI 75 CTTAAG C^TTAAG BpmI 696 CTCCAG CTGGAG(16/14) BsaAI 27 CACGTG [CT]AC^GT[GA] BsaBI 709 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 919 GACGCC G[GA]^CG[CT]C BsaWI 899 TCCGGA [AT]^CCGG[AT] BseAI 899 TCCGGA T^CCGGA BsgI 761 CTGCAC GTGCAG(16/14) BsiHKAI 30 GTGCTC G[AT]GC[AT]^C BsmI 517 GAATGC GAATGC(1/-1) BspEI 899 TCCGGA T^CCGGA BspHI 141 TCATGA T^CATGA BssSI 1331 CTCGTG CACGAG(-5/-1) BstUI 21 CGCG CG^CG DsaI 1117 CCATGG C^C[GA][CT]GG HgaI 919 GACGC GACGC(5/10) Hin1I 919 GACGCC G[GA]^CG[CT]C HindIII 579 AAGCTT A^AGCTT MamI 709 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 615 CAATTG C^AATTG MroI 899 TCCGGA T^CCGGA MunI 615 CAATTG C^AATTG MvnI 21 CGCG CG^CG NciI 813 CCGGG CC^[GC]GG NcoI 1117 CCATGG C^CATGG PacI 184 TTAATTAA TTAAT^TAA PflFI 1243 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1243 GACTC GAGTC(4/5) PmaCI 27 CACGTG CAC^GTG PmlI 27 CACGTG CAC^GTG PshBI 187 ATTAAT AT^TAAT RcaI 141 TCATGA T^CATGA SspI 891 AATATT AAT^ATT Tsp45I 821 GTGAC ^GT[GC]AC Tth111I 1243 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1501 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 1135 GAATTT [GA]^AATT[CT] 1406 AAATTC AfaI 283 GTAC GT^AC 1062 GTAC ApoI 1135 GAATTT [GA]^AATT[CT] 1406 AAATTC AvaII 1114 GGACC G^G[AT]CC 1412 GGTCC BfaI 574 CTAG C^TAG 886 CTAG BmyI 30 GTGCTC G[AGT]GC[ACT]^C 1022 GAGCCC Bsp1286I 30 GTGCTC G[AGT]GC[ACT]^C 1022 GAGCCC BsrDI 651 CATTGC GCAATG(2/0) 62 GCAATG Cac8I 569 GCAAGC GC[ACGT]^[ACGT]GC 928 GCGTGC EcoT14I 626 CCTTGG C^C[AT][AT]GG 1117 CCATGG HapII 813 CCGG C^CGG 900 CCGG HpaII 813 CCGG C^CGG 900 CCGG MaeI 574 CTAG C^TAG 886 CTAG MaeIII 744 GTTAC ^GT[ACGT]AC 821 GTGAC MslI 213 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1126 CATATATATG MspI 813 CCGG C^CGG 900 CCGG MwoI 1150 GCAATTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1336 GCCTCAAACGC RsaI 283 GTAC GT^AC 1062 GTAC StyI 626 CCTTGG C^C[AT][AT]GG 1117 CCATGG TaqI 107 TCGA T^CGA 1075 TCGA TthHB8I 107 TCGA T^CGA 1075 TCGA XcmI 423 CCATTCTATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 522 CCAGGATGACTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AluI AlwI BbsI BbvI BpuAI BsaJI BsmFI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeII MboI MboII MflI MnlI MseI MspA1I MvaI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp509I TspRI XhoII