Restriction map for
pFN21AE3939
(Circular) (Six-base) MAPSORT of: pFN21AE3939.seq Check: 5463 from: 1 to: 6259
pFN21AE3939 6259 bp
With 182 enzymes: SgfI *
March 28, 2012 11:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3439 3602 1937
Size: 1502 163 4594
Fragments arranged by size:
4594 1502 163
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3083 4322 5706 5974 1632
Size: 1451 1239 1384 268 1917
Fragments arranged by size:
1917 1451 1384 1239 268
AflII C'TTAA_G
Cuts at: 820 1017 2080 820
Size: 197 1063 4999
Fragments arranged by size:
4999 1063 197
AflIII A'CryG_T
Cuts at: 1203 2031 5158 6135 1203
Size: 828 3127 977 1327
Fragments arranged by size:
3127 1327 977 828
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2277 3387 1184
Size: 1093 1110 4056
Fragments arranged by size:
4056 1110 1093
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 6227
AlwNI CAG_nnn'CTG
Cuts at: 2717 4002 5574 2717
Size: 1285 1572 3402
Fragments arranged by size:
3402 1572 1285
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 3140 3411 3568 3794 3946 6055 1493
Size: 1647 271 157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1647 271 226 157 152
AseI AT'TA_AT
Cuts at: 160 1032 2193 4813 160
Size: 872 1161 2620 1606
Fragments arranged by size:
2620 1606 1161 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 3584 1870
Size: 73 1641 4545
Fragments arranged by size:
4545 1641 73
BamHI G'GATC_C
Cuts at: 2423 3589 3956 2423
Size: 1166 367 4726
Fragments arranged by size:
4726 1166 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3580 5035 618
Size: 325 217 627 1793 1455 1842
Fragments arranged by size:
1842 1793 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3031 3578 1391
Size: 1640 547 4072
Fragments arranged by size:
4072 1640 547
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 3305 3513 928
Size: 971 335 1071 208 3674
Fragments arranged by size:
3674 1071 971 335 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4450 4484 1903
Size: 34 2513 34 3678
Fragments arranged by size:
3678 2513 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4870 136
Size: 107 122 71 631 605 3198 1525
Fragments arranged by size:
3198 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 4320 5476 1790
Size: 2530 1156 2573
Fragments arranged by size:
2573 2530 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2841 3990 4948
Size: 416 53 83 399 24 1424 1149 958
Cuts at: 4948 442
Size: 1753
Fragments arranged by size:
1753 1424 1149 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2686 4919 1239
Size: 1447 2233 2579
Fragments arranged by size:
2579 2233 1447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2608 2649 4384 5249 5547 2608
Size: 41 1735 865 298 3320
Fragments arranged by size:
3320 1735 865 298 41
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4922 882
Size: 617 3423 2219
Fragments arranged by size:
3423 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2034 4050 493
Size: 711 830 2016 2702
Fragments arranged by size:
2702 2016 830 711
BsaBI GATnn'nnATC
Cuts at: 2718 6253 2718
Size: 3535 2724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2925 4066 4448 5083
Size: 53 83 186 936 1392 1141 382 635
Cuts at: 5083 275
Size: 1451
Fragments arranged by size:
1451 1392 1141 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2904 4692 5090 5364 5511 5922 2904
Size: 1788 398 274 147 411 3241
Fragments arranged by size:
3241 1788 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2400 2430 1178
Size: 30 165 30 997 30 5007
Fragments arranged by size:
5007 997 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6259
BseYI C'CCAG_C
Cuts at: 2306 5462 5958 2306
Size: 3156 496 2607
Fragments arranged by size:
3156 2607 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2751 1163
Size: 573 1015 4671
Fragments arranged by size:
4671 1015 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3709 4470 5498 1540
Size: 275 186 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2039 4320 4405 5476 2039
Size: 2281 85 1071 2822
Fragments arranged by size:
2822 2281 1071 85
BsmI GAATG_Cn'
Cuts at: 2528 3770 3863 2528
Size: 1242 93 4924
Fragments arranged by size:
4924 1242 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 3031 3578 4320 4405 5476 1391
Size: 399 249 992 547 742 85 1071 2174
Fragments arranged by size:
2174 1071 992 742 547 399 249 85
BspEI T'CCGG_A
Cuts at: 2904 5922 2904
Size: 3018 3241
BspHI T'CATG_A
Cuts at: 2146 4148 5878 2146
Size: 2002 1730 2527
Fragments arranged by size:
2527 2002 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3614 844
Size: 2770 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4146 1394
Size: 429 2323 3507
Fragments arranged by size:
3507 2323 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2074 2655 4754 4928 65
Size: 1183 826 581 2099 174 1396
Fragments arranged by size:
2099 1396 1183 826 581 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4903 5090 1700
Size: 110 3093 187 2869
Fragments arranged by size:
3093 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 3336 4319 5331 3336
Size: 983 1012 4264
Fragments arranged by size:
4264 1012 983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6259
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstYI r'GATC_y
Cuts at: 1972 2314 2423 2713 3589 3956 4341 4358 5810
Size: 342 109 290 876 367 385 17 1452
Cuts at: 5810 5896 5908 6254 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 876 385 367 346 342 290
109 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3122 513
Size: 552 939 1118 3650
Fragments arranged by size:
3650 1118 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5520
Fragments arranged by size:
5520 683 56
BtsI GCAGTG_nn'
Cuts at: 2871 3775 4569 4589 2871
Size: 904 794 20 4541
Fragments arranged by size:
4541 904 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2667 2702 529
Size: 35 2103 35 4086
Fragments arranged by size:
4086 2103 35 35
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3706 4595 6224
Size: 54 1425 50 275 18 1876 889 1629
Cuts at: 6224 8
Size: 43
Fragments arranged by size:
1876 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4189 1353
Size: 2836 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4846 5220 5366 387
Size: 1064 138 3257 374 146 1280
Fragments arranged by size:
3257 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2686 3083 4322 4919 5706 5974 1239
Size: 393 1054 397 1239 597 787 268 1524
Fragments arranged by size:
1524 1239 1054 787 597 397 393 268
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 5406 1678
Size: 3728 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 4900 4932 4974
Size: 32 135 32 449 32 2847 32 42
Cuts at: 4974 5006 1373
Size: 32 2626
Fragments arranged by size:
2847 2626 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3603 3849 669
Size: 988 281 1665 246 3079
Fragments arranged by size:
3079 1665 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2584 748
Size: 1836 4423
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 2620 3858 2620
Size: 1238 5021
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2026 5373 5557 1305
Size: 721 3347 184 2007
Fragments arranged by size:
3347 2007 721 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4437
Fragments arranged by size:
4437 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 3135 4217 4576 4735 518
Size: 648 1056 913 1082 359 159 2042
Fragments arranged by size:
2042 1082 1056 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2765 3314 3466 3675 4352 5500 5745 1606
Size: 1159 549 152 209 677 1148 245 2120
Fragments arranged by size:
2120 1159 1148 677 549 245 209 152
NcoI C'CATG_G
Cuts at: 513 1065 2004 3122 513
Size: 552 939 1118 3650
Fragments arranged by size:
3650 1118 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 3617 5162 1132
Size: 97 2388 1545 2229
Fragments arranged by size:
2388 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 2193 2193
Size: 6259
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6259
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6259
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PmlI CAC'GTG
Cuts at: 2034 2034
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 5841 5873 4328
Size: 32 1481 32 4714
Fragments arranged by size:
4714 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PstI C_TGCA'G
Cuts at: 830 3611 830
Size: 2781 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 2765 3314 3466 2765
Size: 549 152 5558
Fragments arranged by size:
5558 549 152
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SbfI CC_TGCA'GG
Cuts at: 3611 3611
Size: 6259
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 2040 2169 2914 3359 3607 4742 5423
Size: 219 995 129 745 445 248 1135 681
Cuts at: 5423 5614 826
Size: 191 1471
Fragments arranged by size:
1471 1135 995 745 681 445 248 219
191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 3586 1872
Size: 1714 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 2587 2664 4363 5264 5526
Size: 197 926 137 507 77 1699 901 262
Cuts at: 5526 820
Size: 1553
Fragments arranged by size:
1699 1553 926 901 507 262 197 137
77
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3617 1132
Size: 2485 3774
SspI AAT'ATT
Cuts at: 5 52 2898 4183 5
Size: 47 2846 1285 2081
Fragments arranged by size:
2846 2081 1285 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6259
StyI C'CwwG_G
Cuts at: 513 1065 2004 2631 3122 3698 513
Size: 552 939 627 491 576 3074
Fragments arranged by size:
3074 939 627 576 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3405 4286 4303 4456 4641 5086 325
Size: 1255 1825 881 17 153 185 445 1498
Fragments arranged by size:
1825 1498 1255 881 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4505 6217 96
Size: 274 80 33 51 1443 2528 1712 138
Fragments arranged by size:
2528 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 3251 1316
Size: 1935 4324
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2435 2534 2435
Size: 99 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 3584 1870
Size: 1714 4545
XmnI GAAnn'nnTTC
Cuts at: 3510 4388 3510
Size: 878 5381
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI