(Circular) (Six-base) MAPSORT of: pFN21AB9933.seq Check: 3811 from: 1 to: 6172 pFN21AB9933 6172 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2850 3515 1937 Size: 913 665 4594 Fragments arranged by size: 4594 913 665 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2704 3236 4235 5619 5887 1632 Size: 1072 532 999 1384 268 1917 Fragments arranged by size: 1917 1384 1072 999 532 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 2928 3234 5071 6048 1203 Size: 1725 306 1837 977 1327 Fragments arranged by size: 1837 1725 1327 977 306 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 2633 2843 1166 Size: 1467 210 4495 Fragments arranged by size: 4495 1467 210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 2794 3382 3915 5487 2794 Size: 588 533 1572 3479 Fragments arranged by size: 3479 1572 588 533 ApaI G_GGCC'C Cuts at: 2668 2815 3114 3289 3391 2668 Size: 147 299 175 102 5449 Fragments arranged by size: 5449 299 175 147 102 ApaLI G'TGCA_C Cuts at: 4229 5385 4229 Size: 1156 5016 ApoI r'AATT_y Cuts at: 1493 2036 3481 3707 3859 5968 1493 Size: 543 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2768 2800 3113 3265 3497 1870 Size: 73 825 32 313 152 232 4545 Fragments arranged by size: 4545 825 313 232 152 73 32 AvrII C'CTAG_G Cuts at: 3280 3280 Size: 6172 BamHI G'GATC_C Cuts at: 3502 3869 3502 Size: 367 5805 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2424 2627 3493 4948 Size: 325 217 627 390 247 203 866 1455 Cuts at: 4948 618 Size: 1842 Fragments arranged by size: 1842 1455 866 627 390 325 247 217 203 BanII G_rGCy'C Cuts at: 1391 2668 2815 3045 3114 3272 3289 3391 3491 Size: 1277 147 230 69 158 17 102 100 Cuts at: 3491 1391 Size: 4072 Fragments arranged by size: 4072 1277 230 158 147 102 100 69 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5201 BbvCI CC'TCA_GC Cuts at: 3132 3132 Size: 6172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 1903 Size: 34 2426 34 3678 Fragments arranged by size: 3678 2426 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3128 3350 3395 Size: 107 122 71 631 605 1456 222 45 Cuts at: 3395 4783 136 Size: 1388 1525 Fragments arranged by size: 1525 1456 1388 631 605 222 122 107 71 45 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 2581 3589 2581 Size: 1008 5164 Bme1580I G_kGCm'C Cuts at: 1790 2247 2397 2495 2630 2668 2815 3114 3198 Size: 457 150 98 135 38 147 299 84 Cuts at: 3198 3289 3391 4233 5389 1790 Size: 91 102 842 1156 2573 Fragments arranged by size: 2573 1156 842 457 299 150 147 135 102 98 91 84 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2857 3005 3146 3383 3455 4832 1239 Size: 1169 449 148 141 237 72 1377 2579 Fragments arranged by size: 2579 1377 1169 449 237 148 141 72 Bpu10I CC'TnA_GC Cuts at: 2198 3132 2198 Size: 934 5238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4835 882 Size: 617 3336 2219 Fragments arranged by size: 3336 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3193 3963 493 Size: 711 1989 770 2702 Fragments arranged by size: 2702 1989 770 711 BsaBI GATnn'nnATC Cuts at: 3170 6166 3170 Size: 2996 3176 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3979 4361 4996 275 Size: 53 83 186 936 2446 382 635 1451 Fragments arranged by size: 2446 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 4605 5003 5277 5424 5835 2880 Size: 1725 398 274 147 411 3217 Fragments arranged by size: 3217 1725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 4828 Fragments arranged by size: 4828 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 2915 3149 3262 3380 3452 1186 Size: 1330 399 234 113 118 72 3906 Fragments arranged by size: 3906 1330 399 234 118 113 72 BseYI C'CCAG_C Cuts at: 3197 3323 3353 3445 5375 5871 3197 Size: 126 30 92 1930 496 3498 Fragments arranged by size: 3498 1930 496 126 92 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2302 2475 2510 1163 Size: 573 566 173 35 4825 Fragments arranged by size: 4825 573 566 173 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 3622 4383 5411 1540 Size: 275 186 1420 201 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 201 186 BsiHKAI G_wGCw'C Cuts at: 2193 4233 4318 5389 2193 Size: 2040 85 1071 2976 Fragments arranged by size: 2976 2040 1071 85 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2247 2397 2495 2630 2668 2815 Size: 399 403 54 150 98 135 38 147 Cuts at: 2815 3045 3114 3198 3272 3289 3391 3491 4233 Size: 230 69 84 74 17 102 100 742 Cuts at: 4233 4318 5389 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 230 150 147 135 102 100 98 85 84 74 69 54 38 17 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 2656 3051 4061 5791 2656 Size: 395 1010 1730 3037 Fragments arranged by size: 3037 1730 1010 395 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3527 844 Size: 2683 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2644 2737 3147 4059 1394 Size: 429 821 93 410 912 3507 Fragments arranged by size: 3507 912 821 429 410 93 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2374 3225 3249 4816 5003 1700 Size: 110 249 315 851 24 1567 187 2869 Fragments arranged by size: 2869 1567 851 315 249 187 110 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6172 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 2124 3382 794 Size: 1330 1258 3584 Fragments arranged by size: 3584 1330 1258 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2407 2689 2146 Size: 261 282 5629 Fragments arranged by size: 5629 282 261 BstYI r'GATC_y Cuts at: 1972 2337 3502 3869 4254 4271 5723 5809 5821 Size: 365 1165 367 385 17 1452 86 12 Cuts at: 5821 6167 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1165 385 367 365 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3392 3392 Size: 6172 BtgI C'CryG_G Cuts at: 513 1065 2430 2778 3258 513 Size: 552 1365 348 480 3427 Fragments arranged by size: 3427 1365 552 480 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2401 487 Size: 683 56 1175 4258 Fragments arranged by size: 4258 1175 683 56 BtsI GCAGTG_nn' Cuts at: 3176 3303 3688 4482 4502 3176 Size: 127 385 794 20 4846 Fragments arranged by size: 4846 794 385 127 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2321 5179 809 Size: 507 1005 2858 1802 Fragments arranged by size: 2858 1802 1005 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3336 3619 4508 Size: 54 1425 50 275 18 1506 283 889 Cuts at: 4508 6137 8 Size: 1629 43 Fragments arranged by size: 1629 1506 1425 889 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3619 1537 Size: 275 1807 4090 Fragments arranged by size: 4090 1807 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3083 4102 1353 Size: 1730 1019 3423 Fragments arranged by size: 3423 1730 1019 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2704 2857 3005 3146 3236 3383 Size: 393 776 296 153 148 141 90 147 Cuts at: 3383 3455 4235 4832 5619 5887 1239 Size: 72 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 776 597 393 296 268 153 148 147 141 90 72 EcoNI CCTnn'n_nnAGG Cuts at: 3080 3080 Size: 6172 EcoO109I rG'GnC_Cy Cuts at: 2252 2664 2755 2811 2812 2980 3211 3285 3286 Size: 412 91 56 1 168 231 74 1 Cuts at: 3286 3388 2252 Size: 102 5036 Fragments arranged by size: 5036 412 231 168 102 91 74 56 1 1 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FspI TGC'GCA Cuts at: 2606 4678 2606 Size: 2072 4100 FspAI rTGC'GCAy Cuts at: 2606 2606 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2750 3401 5319 1678 Size: 1072 651 1918 2531 Fragments arranged by size: 2531 1918 1072 651 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 4813 4845 4887 Size: 32 135 32 825 32 2384 32 42 Cuts at: 4887 4919 1373 Size: 32 2626 Fragments arranged by size: 2626 2384 825 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3516 3762 669 Size: 988 281 196 1382 246 3079 Fragments arranged by size: 3079 1382 988 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 5286 5470 1305 Size: 1746 2235 184 2007 Fragments arranged by size: 2235 2007 1746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3338 10 Size: 54 1425 343 1506 2844 Fragments arranged by size: 2844 1506 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2324 2633 2843 3050 3056 4130 Size: 648 927 231 309 210 207 6 1074 Cuts at: 4130 4489 4648 518 Size: 359 159 2042 Fragments arranged by size: 2042 1074 927 648 359 309 231 210 207 159 6 MspA1I CmG'CkG Cuts at: 1606 2304 2365 2498 2553 2743 2953 3208 3327 Size: 698 61 133 55 190 210 255 119 Cuts at: 3327 3445 3588 4265 5413 5658 1606 Size: 118 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 255 245 210 190 143 133 119 118 61 55 NaeI GCC'GGC Cuts at: 3227 3251 3227 Size: 24 6148 NcoI C'CATG_G Cuts at: 513 1065 2778 3258 513 Size: 552 1713 480 3427 Fragments arranged by size: 3427 1713 552 480 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NgoMIV G'CCGG_C Cuts at: 3225 3249 3225 Size: 24 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2048 2589 2971 3530 5075 1132 Size: 97 819 541 382 559 1545 2229 Fragments arranged by size: 2229 1545 819 559 541 382 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6172 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6172 PfoI T'CCnGG_A Cuts at: 2259 3081 2259 Size: 822 5350 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2252 2755 3211 2252 Size: 503 456 5213 Fragments arranged by size: 5213 503 456 PshAI GACnn'nnGTC Cuts at: 1613 2274 2619 1613 Size: 661 345 5166 Fragments arranged by size: 5166 661 345 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PspOMI G'GGCC_C Cuts at: 2664 2811 3110 3285 3387 2664 Size: 147 299 175 102 5449 Fragments arranged by size: 5449 299 175 147 102 PspXI vC'TCGA_Gb Cuts at: 2768 2800 2768 Size: 32 6140 PstI C_TGCA'G Cuts at: 830 2790 3524 830 Size: 1960 734 3478 Fragments arranged by size: 3478 1960 734 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2553 2743 3445 2553 Size: 190 702 5280 Fragments arranged by size: 5280 702 190 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SanDI GG'GwC_CC Cuts at: 2755 2755 Size: 6172 SbfI CC_TGCA'GG Cuts at: 2790 3524 2790 Size: 734 5438 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 2786 3520 4655 5336 5527 826 Size: 219 1741 734 1135 681 191 1471 Fragments arranged by size: 1741 1471 1135 734 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 3267 3499 1872 Size: 1395 232 4545 Fragments arranged by size: 4545 1395 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 2800 4276 5177 5439 820 Size: 197 926 825 32 1476 901 262 1553 Fragments arranged by size: 1553 1476 926 901 825 262 197 32 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 2589 2971 3530 1132 Size: 1457 382 559 3774 Fragments arranged by size: 3774 1457 559 382 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 2225 3188 1517 Size: 708 963 4501 Fragments arranged by size: 4501 963 708 StyI C'CwwG_G Cuts at: 513 1065 2778 3258 3280 3611 513 Size: 552 1713 480 22 331 3074 Fragments arranged by size: 3074 1713 552 480 331 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3130 4199 4216 4369 4554 4999 325 Size: 1255 1550 1069 17 153 185 445 1498 Fragments arranged by size: 1550 1498 1255 1069 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4418 6130 96 Size: 274 80 33 51 1443 2441 1712 138 Fragments arranged by size: 2441 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 2291 1316 Size: 975 5197 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 3331 2980 Size: 351 5821 XhoI C'TCGA_G Cuts at: 1943 2768 2800 1943 Size: 825 32 5315 Fragments arranged by size: 5315 825 32 XmaI C'CCGG_G Cuts at: 1870 3265 3497 1870 Size: 1395 232 4545 Fragments arranged by size: 4545 1395 232 XmnI GAAnn'nnTTC Cuts at: 2930 4301 2930 Size: 1371 4801 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI