################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI SwaI Tsp509I Tth111I Van91I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 373 GACGTC GACGT^C AlwI 504 GATCC GGATC(4/5) AocI 636 CCTGAGG CC^T[ACGT]AGG ApaI 1183 GGGCCC GGGCC^C AvaI 601 CCCGGG C^[CT]CG[GA]G BcgI 1188 CGACGGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1185 GCCCGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 670 CTGCAC GTGCAG(16/14) BsmBI 371 GAGACG CGTCTC(1/5) BsmI 748 GCATTC GAATGC(1/-1) BsoBI 601 CCCGGG C^[CT]CG[GA]G BsrDI 279 GCAATG GCAATG(2/0) BsrFI 1051 GCCGGC [GA]^CCGG[CT] BstF5I 477 CATCC GGATG(2/0) BstYI 503 AGATCC [GA]^GATC[CT] Bsu36I 636 CCTGAGG CC^T[ACGT]AGG Cfr10I 1051 GCCGGC [GA]^CCGG[CT] DpnII 504 GATC ^GATC DraII 716 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 868 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 660 CGGCCG C^GGCCG EarI 103 CTCTTC CTCTTC(1/4) EclXI 660 CGGCCG C^GGCCG Eco52I 660 CGGCCG C^GGCCG Eco81I 636 CCTGAGG CC^T[ACGT]AGG EcoO109I 716 AGGCCCC [GA]G^G[ACGT]CC[CT] FokI 477 CATCC GGATG(9/13) HphI 681 TCACC GGTGA(8/7) Ksp632I 103 CTCTTC CTCTTC(1/4) KspI 521 CCGCGG CCGC^GG MboI 504 GATC ^GATC MflI 503 AGATCC [GA]^GATC[CT] NaeI 1051 GCCGGC GCC^GGC NcoI 274 CCATGG C^CATGG NdeII 504 GATC ^GATC NgoMIV 1051 GCCGGC G^CCGGC NotI 659 GCGGCCGC GC^GGCCGC NspI 1194 GCATGC [GA]CATG^[CT] PshAI 428 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 521 CCGCGG CCGC^GG Sau3AI 504 GATC ^GATC SfaNI 536 GCATC GCATC(5/9) SmaI 601 CCCGGG CCC^GGG SphI 1194 GCATGC GCATG^C StuI 952 AGGCCT AGG^CCT XbaI 417 TCTAGA T^CTAGA XhoII 503 AGATCC [GA]^GATC[CT] XmaCI 601 CCCGGG C^CCGGG XmaI 601 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 376 GTCTAC GT^[AC][GT]AC 802 GTCTAC BalI 68 TGGCCA TGG^CCA 686 TGGCCA BanII 6 GGGCTC G[GA]GC[CT]^C 1183 GGGCCC BbeI 525 GGCGCC GGCGC^C 1163 GGCGCC BfaI 418 CTAG C^TAG 1147 CTAG BsaI 895 GAGACC GGTCTC(1/5) 1209 GAGACC BsiEI 489 CGACCG CG[GA][CT]^CG 660 CGGCCG Bsp1407I 313 TGTACA T^GTACA 842 TGTACA BsrBI 180 GAGCGG CCGCTC(-3/-3) 657 GAGCGG BsrGI 313 TGTACA T^GTACA 842 TGTACA EcoT14I 167 CCTTGG C^C[AT][AT]GG 274 CCATGG KasI 525 GGCGCC G^GCGCC 1163 GGCGCC MaeI 418 CTAG C^TAG 1147 CTAG MluNI 68 TGGCCA TGG^CCA 686 TGGCCA MscI 68 TGGCCA TGG^CCA 686 TGGCCA MseI 232 TTAA T^TAA 997 TTAA NarI 525 GGCGCC GG^CGCC 1163 GGCGCC SfoI 525 GGCGCC GGC^GCC 1163 GGCGCC SspBI 313 TGTACA T^GTACA 842 TGTACA StyI 167 CCTTGG C^C[AT][AT]GG 274 CCATGG Tru9I 232 TTAA T^TAA 997 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AspHI AvaII BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI PvuII RsaI Sau96I ScrFI SfcI TaqI TfiI TseI Tsp45I TspRI TthHB8I