(Circular) (Six-base) MAPSORT of: pFN21AE1441.seq Check: 8143 from: 1 to: 5941 pFN21AE1441 5941 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2382 3751 278 Size: 53 83 186 936 846 1369 2468 Fragments arranged by size: 2468 1369 936 846 186 83 53 AccI GT'mk_AC Cuts at: 1937 2382 2808 3284 1937 Size: 445 426 476 4594 Fragments arranged by size: 4594 476 445 426 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3058 3140 4004 5388 5656 1632 Size: 1426 82 864 1384 268 1917 Fragments arranged by size: 1917 1426 1384 864 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2410 2412 2801 1166 Size: 1244 2 389 4306 Fragments arranged by size: 4306 1244 389 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2776 1184 Size: 1592 4349 AlwNI CAG_nnn'CTG Cuts at: 2707 2860 2911 3684 5256 2707 Size: 153 51 773 1572 3392 Fragments arranged by size: 3392 1572 773 153 51 ApaI G_GGCC'C Cuts at: 3192 3192 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2606 3266 1870 Size: 73 663 660 4545 Fragments arranged by size: 4545 663 660 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2530 2593 2636 2760 3168 Size: 325 217 627 743 63 43 124 408 Cuts at: 3168 3262 4717 618 Size: 94 1455 1842 Fragments arranged by size: 1842 1455 743 627 408 325 217 124 94 63 43 BanII G_rGCy'C Cuts at: 1391 2015 3192 3260 1391 Size: 624 1177 68 4072 Fragments arranged by size: 4072 1177 624 68 BbeI G_GCGC'C Cuts at: 2534 3172 2534 Size: 638 5303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3182 3216 4132 4166 1903 Size: 34 1245 34 916 34 3678 Fragments arranged by size: 3678 1245 916 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3196 4552 136 Size: 107 122 71 631 605 1524 1356 1525 Fragments arranged by size: 1525 1524 1356 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2565 3192 4002 5158 1790 Size: 775 627 810 1156 2573 Fragments arranged by size: 2573 1156 810 775 627 BmgBI CAC'GTC Cuts at: 1318 2887 1318 Size: 1569 4372 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3043 3672 4630 Size: 416 53 83 399 24 1626 629 958 Cuts at: 4630 442 Size: 1753 Fragments arranged by size: 1753 1626 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2394 2420 2906 4601 1239 Size: 1155 26 486 1695 2579 Fragments arranged by size: 2579 1695 1155 486 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2894 3208 4604 882 Size: 617 1395 314 1396 2219 Fragments arranged by size: 2219 1396 1395 617 314 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2201 2379 2531 2826 Size: 53 83 186 936 668 178 152 295 Cuts at: 2826 3066 3169 3748 4130 4765 275 Size: 240 103 579 382 635 1451 Fragments arranged by size: 1451 936 668 635 579 382 295 240 186 178 152 103 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 3095 3125 1178 Size: 30 165 30 966 30 696 30 3994 Fragments arranged by size: 3994 966 696 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2360 2614 2912 3146 1186 Size: 1174 254 298 234 3981 Fragments arranged by size: 3981 1174 298 254 234 BseYI C'CCAG_C Cuts at: 2428 2520 5144 5640 2428 Size: 92 2624 496 2729 Fragments arranged by size: 2729 2624 496 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2660 1163 Size: 573 924 4444 Fragments arranged by size: 4444 924 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2497 2668 3391 4152 5180 1540 Size: 275 186 496 171 723 761 1028 2301 Fragments arranged by size: 2301 1028 761 723 496 275 186 171 BsiHKAI G_wGCw'C Cuts at: 2922 2952 2996 4002 4087 5158 2922 Size: 30 44 1006 85 1071 3705 Fragments arranged by size: 3705 1071 1006 85 44 30 BsmI GAATG_Cn' Cuts at: 2753 3452 3545 2753 Size: 699 93 5149 Fragments arranged by size: 5149 699 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2370 2370 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2565 2922 2952 2996 3192 3260 Size: 399 225 550 357 30 44 196 68 Cuts at: 3260 4002 4087 5158 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 399 357 225 196 85 68 44 30 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2187 2664 3828 1394 Size: 429 364 477 1164 3507 Fragments arranged by size: 3507 1164 477 429 364 BsrDI GCAATG_nn' Cuts at: 65 1248 2291 4436 4610 65 Size: 1183 1043 2145 174 1396 Fragments arranged by size: 2145 1396 1183 1043 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3056 4585 4772 1700 Size: 110 1246 1529 187 2869 Fragments arranged by size: 2869 1529 1246 187 110 BsrGI T'GTAC_A Cuts at: 96 2318 2847 96 Size: 2222 529 3190 Fragments arranged by size: 3190 2222 529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2508 3271 3638 4023 4040 5492 5578 5590 Size: 536 763 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 763 536 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2642 2642 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2004 2279 2304 2526 2589 513 Size: 552 939 275 25 222 63 3865 Fragments arranged by size: 3865 939 552 275 222 63 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2098 2131 2487 2677 3457 4251 4271 2098 Size: 33 356 190 780 794 20 3768 Fragments arranged by size: 3768 794 780 356 190 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 2878 2878 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2361 2416 Size: 54 1425 50 275 18 243 288 55 Cuts at: 2416 2665 2691 3388 4277 5906 8 Size: 249 26 697 889 1629 43 Fragments arranged by size: 1629 1425 889 697 288 275 249 243 55 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2665 3388 1537 Size: 275 853 723 4090 Fragments arranged by size: 4090 853 723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 3871 1353 Size: 761 1757 3423 Fragments arranged by size: 3423 1757 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2394 2420 2906 3058 3140 4004 4601 Size: 393 762 26 486 152 82 864 597 Cuts at: 4601 5388 5656 1239 Size: 787 268 1524 Fragments arranged by size: 1524 864 787 762 597 486 393 268 152 82 26 EcoO109I rG'GnC_Cy Cuts at: 2722 2722 Size: 5941 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2534 2783 3172 5088 1678 Size: 856 249 389 1916 2531 Fragments arranged by size: 2531 1916 856 389 249 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 4582 4614 4656 Size: 32 135 32 797 32 2181 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 2181 797 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2530 3168 2530 Size: 638 5303 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2693 10 Size: 54 1425 343 243 618 3258 Fragments arranged by size: 3258 1425 618 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2284 2410 2412 2758 2801 3899 4258 Size: 648 1118 126 2 346 43 1098 359 Cuts at: 4258 4417 518 Size: 159 2042 Fragments arranged by size: 2042 1118 1098 648 359 346 159 126 43 2 MspA1I CmG'CkG Cuts at: 1606 2520 2528 2674 2821 2860 3166 3357 4034 Size: 914 8 146 147 39 306 191 677 Cuts at: 4034 5182 5427 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 914 677 306 245 191 147 146 39 8 NaeI GCC'GGC Cuts at: 3058 3058 Size: 5941 NarI GG'CG_CC Cuts at: 2531 3169 2531 Size: 638 5303 NcoI C'CATG_G Cuts at: 513 1065 2004 2279 513 Size: 552 939 275 4175 Fragments arranged by size: 4175 939 552 275 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 3056 3056 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 2665 3388 2665 Size: 723 5218 NspI r_CATG'y Cuts at: 1132 1229 3203 3299 4844 1132 Size: 97 1974 96 1545 2229 Fragments arranged by size: 2229 1974 1545 97 96 PasI CC'CwG_GG Cuts at: 1348 2905 1348 Size: 1557 4384 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PfoI T'CCnGG_A Cuts at: 2744 2922 2744 Size: 178 5763 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2437 1613 Size: 824 5117 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 3188 3188 Size: 5941 PstI C_TGCA'G Cuts at: 830 2126 2134 2703 3144 3293 830 Size: 1296 8 569 441 149 3478 Fragments arranged by size: 3478 1296 569 441 149 8 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2520 2674 2821 2860 2520 Size: 154 147 39 5601 Fragments arranged by size: 5601 154 147 39 SacII CC_GC'GG Cuts at: 2529 2529 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2122 2130 2699 2953 3140 3289 4424 Size: 219 1077 8 569 254 187 149 1135 Cuts at: 4424 5105 5296 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1077 681 569 254 219 191 187 149 8 SfoI GGC'GCC Cuts at: 2532 3170 2532 Size: 638 5303 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2608 3268 1872 Size: 736 660 4545 Fragments arranged by size: 4545 736 660 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3203 3299 1132 Size: 2071 96 3774 Fragments arranged by size: 3774 2071 96 SrfI GCCC'GGGC Cuts at: 2608 2608 Size: 5941 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2959 1517 Size: 1442 4499 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 2279 3380 513 Size: 552 939 168 107 1101 3074 Fragments arranged by size: 3074 1101 939 552 168 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2905 3968 3985 4138 4323 4768 325 Size: 1255 1325 1063 17 153 185 445 1498 Fragments arranged by size: 1498 1325 1255 1063 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2318 2655 2847 Size: 274 80 33 51 1443 341 337 192 Cuts at: 2847 4187 5899 96 Size: 1340 1712 138 Fragments arranged by size: 1712 1443 1340 341 337 274 192 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 2422 3277 2422 Size: 855 5086 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2606 3266 1870 Size: 736 660 4545 Fragments arranged by size: 4545 736 660 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2380 3749 276 Size: 53 83 186 936 846 1369 2468 Fragments arranged by size: 2468 1369 936 846 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI