Restriction map for
pFN21AE1441
(Linear) (Six-base) MAPSORT of: KIEE1441.seq Check: 8553 from: 1 to: 1236
KIEE1441.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AatII G_ACGT'C
Cuts at: 0 377 1236
Size: 377 859
AccI GT'mk_AC
Cuts at: 0 377 803 1236
Size: 377 426 433
Fragments arranged by size:
433 426 377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1053 1135 1236
Size: 1053 82 101
Fragments arranged by size:
1053 101 82
AleI CACnn'nnGTG
Cuts at: 0 405 407 796 1236
Size: 405 2 389 440
Fragments arranged by size:
440 405 389 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 771 1236
Size: 771 465
AlwNI CAG_nnn'CTG
Cuts at: 0 702 855 906 1236
Size: 702 153 51 330
Fragments arranged by size:
702 330 153 51
ApaI G_GGCC'C
Cuts at: 0 1187 1236
Size: 1187 49
AvaI C'yCGr_G
Cuts at: 0 601 1236
Size: 601 635
BanI G'GyrC_C
Cuts at: 0 525 588 631 755 1163 1236
Size: 525 63 43 124 408 73
Fragments arranged by size:
525 408 124 73 63 43
BanII G_rGCy'C
Cuts at: 0 10 1187 1236
Size: 10 1177 49
Fragments arranged by size:
1177 49 10
BbeI G_GCGC'C
Cuts at: 0 529 1167 1236
Size: 529 638 69
Fragments arranged by size:
638 529 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1177 1211 1236
Size: 1177 34 25
Fragments arranged by size:
1177 34 25
BglI GCCn_nnn'nGGC
Cuts at: 0 1191 1236
Size: 1191 45
Bme1580I G_kGCm'C
Cuts at: 0 560 1187 1236
Size: 560 627 49
Fragments arranged by size:
627 560 49
BmgBI CAC'GTC
Cuts at: 0 882 1236
Size: 882 354
BmrI ACTGGGnnnn_n'
Cuts at: 0 1038 1236
Size: 1038 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 415 901 1236
Size: 389 26 486 335
Fragments arranged by size:
486 389 335 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 889 1203 1236
Size: 889 314 33
Fragments arranged by size:
889 314 33
BsaHI Gr'CG_yC
Cuts at: 0 196 374 526 821 1061 1164 1236
Size: 196 178 152 295 240 103 72
Fragments arranged by size:
295 240 196 178 152 103 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 1090 1120 1236
Size: 364 30 696 30 116
Fragments arranged by size:
696 364 116 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 355 609 907 1141 1236
Size: 355 254 298 234 95
Fragments arranged by size:
355 298 254 234 95
BseYI C'CCAG_C
Cuts at: 0 423 515 1236
Size: 423 92 721
Fragments arranged by size:
721 423 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1236
Size: 655 581
BsiEI CG_ry'CG
Cuts at: 0 492 663 1236
Size: 492 171 573
Fragments arranged by size:
573 492 171
BsiHKAI G_wGCw'C
Cuts at: 0 917 947 991 1236
Size: 917 30 44 245
Fragments arranged by size:
917 245 44 30
BsmI GAATG_Cn'
Cuts at: 0 748 1236
Size: 748 488
BsmBI CGTCTCn'nnnn_
Cuts at: 0 365 1236
Size: 365 871
Bsp1286I G_dGCh'C
Cuts at: 0 10 560 917 947 991 1187 1236
Size: 10 550 357 30 44 196 49
Fragments arranged by size:
550 357 196 49 44 30 10
BsrBI CCG'CTC
Cuts at: 0 182 659 1236
Size: 182 477 577
Fragments arranged by size:
577 477 182
BsrDI GCAATG_nn'
Cuts at: 0 286 1236
Size: 286 950
BsrFI r'CCGG_y
Cuts at: 0 1051 1236
Size: 1051 185
BsrGI T'GTAC_A
Cuts at: 0 313 842 1236
Size: 313 529 394
Fragments arranged by size:
529 394 313
BstYI r'GATC_y
Cuts at: 0 503 1236
Size: 503 733
Bsu36I CC'TnA_GG
Cuts at: 0 637 1236
Size: 637 599
BtgI C'CryG_G
Cuts at: 0 274 299 521 584 1236
Size: 274 25 222 63 652
Fragments arranged by size:
652 274 222 63 25
BtsI GCAGTG_nn'
Cuts at: 0 93 126 482 672 1236
Size: 93 33 356 190 564
Fragments arranged by size:
564 356 190 93 33
DraIII CAC_nnn'GTG
Cuts at: 0 873 1236
Size: 873 363
EaeI y'GGCC_r
Cuts at: 0 68 356 411 660 686 1236
Size: 68 288 55 249 26 550
Fragments arranged by size:
550 288 249 68 55 26
EagI C'GGCC_G
Cuts at: 0 660 1236
Size: 660 576
EarI CTCTTCn'nnn_
Cuts at: 0 109 1236
Size: 109 1127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 415 901 1053 1135 1236
Size: 389 26 486 152 82 101
Fragments arranged by size:
486 389 152 101 82 26
EcoO109I rG'GnC_Cy
Cuts at: 0 717 1236
Size: 717 519
HaeII r_GCGC'y
Cuts at: 0 529 778 1167 1236
Size: 529 249 389 69
Fragments arranged by size:
529 389 249 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 1236
Size: 364 32 840
Fragments arranged by size:
840 364 32
KasI G'GCGC_C
Cuts at: 0 525 1163 1236
Size: 525 638 73
Fragments arranged by size:
638 525 73
MscI TGG'CCA
Cuts at: 0 70 688 1236
Size: 70 618 548
Fragments arranged by size:
618 548 70
MslI CAynn'nnrTG
Cuts at: 0 279 405 407 753 796 1236
Size: 279 126 2 346 43 440
Fragments arranged by size:
440 346 279 126 43 2
MspA1I CmG'CkG
Cuts at: 0 515 523 669 816 855 1161 1236
Size: 515 8 146 147 39 306 75
Fragments arranged by size:
515 306 147 146 75 39 8
NaeI GCC'GGC
Cuts at: 0 1053 1236
Size: 1053 183
NarI GG'CG_CC
Cuts at: 0 526 1164 1236
Size: 526 638 72
Fragments arranged by size:
638 526 72
NcoI C'CATG_G
Cuts at: 0 274 1236
Size: 274 962
NgoMIV G'CCGG_C
Cuts at: 0 1051 1236
Size: 1051 185
NotI GC'GGCC_GC
Cuts at: 0 660 1236
Size: 660 576
NspI r_CATG'y
Cuts at: 0 1198 1236
Size: 1198 38
PasI CC'CwG_GG
Cuts at: 0 900 1236
Size: 900 336
PfoI T'CCnGG_A
Cuts at: 0 739 917 1236
Size: 739 178 319
Fragments arranged by size:
739 319 178
PshAI GACnn'nnGTC
Cuts at: 0 432 1236
Size: 432 804
PspOMI G'GGCC_C
Cuts at: 0 1183 1236
Size: 1183 53
PstI C_TGCA'G
Cuts at: 0 121 129 698 1139 1236
Size: 121 8 569 441 97
Fragments arranged by size:
569 441 121 97 8
PvuII CAG'CTG
Cuts at: 0 515 669 816 855 1236
Size: 515 154 147 39 381
Fragments arranged by size:
515 381 154 147 39
SacII CC_GC'GG
Cuts at: 0 524 1236
Size: 524 712
SfcI C'TryA_G
Cuts at: 0 117 125 694 948 1135 1236
Size: 117 8 569 254 187 101
Fragments arranged by size:
569 254 187 117 101 8
SfoI GGC'GCC
Cuts at: 0 527 1165 1236
Size: 527 638 71
Fragments arranged by size:
638 527 71
SmaI CCC'GGG
Cuts at: 0 603 1236
Size: 603 633
SphI G_CATG'C
Cuts at: 0 1198 1236
Size: 1198 38
SrfI GCCC'GGGC
Cuts at: 0 603 1236
Size: 603 633
StuI AGG'CCT
Cuts at: 0 954 1236
Size: 954 282
StyI C'CwwG_G
Cuts at: 0 167 274 1236
Size: 167 107 962
Fragments arranged by size:
962 167 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 900 1236
Size: 900 336
TatI w'GTAC_w
Cuts at: 0 313 650 842 1236
Size: 313 337 192 394
Fragments arranged by size:
394 337 313 192
XbaI T'CTAG_A
Cuts at: 0 417 1236
Size: 417 819
XmaI C'CCGG_G
Cuts at: 0 601 1236
Size: 601 635
ZraI GAC'GTC
Cuts at: 0 375 1236
Size: 375 861
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BcgI BglI Bme1580I BmgBI BmrI
BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PfoI
PshAI PspOMI PstI PvuII SacII SfcI SfoI SmaI
SphI SrfI StuI StyI TaqII TatI XbaI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI
BspEI BspHI BspMI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SspI SwaI TstI Tth111I
XcmI XhoI XmnI