Restriction map for
pFN21AB7921
##################################
# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AfaI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 111 GACGCC G[GA]^CG[CT]C
AflIII 454 ACGTGT A^C[GA][CT]GT
AlwNI 191 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 400 GAGCTC G[AT]GC[AT]^C
AvaI 214 CTCGGG C^[CT]CG[GA]G
BbrPI 453 CACGTG CAC^GTG
BbsI 327 GAAGAC GAAGAC(2/6)
BbvCI 132 GCTGAGG CCTCAGC(-5/-2)
BcnI 465 CCGGG CC^[GC]GG
BpmI 485 CTCCAG CTGGAG(16/14)
BpuAI 327 GAAGAC GAAGAC(2/6)
BsaAI 453 CACGTG [CT]AC^GT[GA]
BsaHI 111 GACGCC G[GA]^CG[CT]C
BsaI 24 GGTCTC GGTCTC(1/5)
BsaWI 167 ACCGGA [AT]^CCGG[AT]
BseRI 397 GAGGAG GAGGAG(10/8)
BsiHKAI 400 GAGCTC G[AT]GC[AT]^C
BsmAI 25 GTCTC GTCTC(1/5)
BsmI 372 GAATGC GAATGC(1/-1)
BsoBI 214 CTCGGG C^[CT]CG[GA]G
BsrDI 533 CATTGC GCAATG(2/0)
BsrI 84 ACTGG ACTGG(1/-1)
Cac8I 338 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 181 GCGC GCG^C
Cfr13I 449 GGCCC G^G[ACGT]CC
EarI 127 GAAGAG CTCTTC(1/4)
HgaI 111 GACGC GACGC(5/10)
HhaI 181 GCGC GCG^C
Hin1I 111 GACGCC G[GA]^CG[CT]C
HinP1I 181 GCGC G^CGC
Ksp632I 127 GAAGAG CTCTTC(1/4)
MaeII 454 ACGT A^CGT
MspA1I 296 CAGCTG C[AC]G^C[GT]G
MwoI 235 GCCGAGGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 465 CCGGG CC^[GC]GG
NlaIII 432 CATG CATG^
NspI 431 ACATGC [GA]CATG^[CT]
PmaCI 453 CACGTG CAC^GTG
PmlI 453 CACGTG CAC^GTG
PstI 340 CTGCAG CTGCA^G
PvuII 296 CAGCTG CAG^CTG
SacI 400 GAGCTC GAGCT^C
SapI 127 GAAGAGC GCTCTTC(1/4)
Sau96I 449 GGCCC G^G[ACGT]CC
SfaNI 3 GATGC GCATC(5/9)
SfcI 340 CTGCAG C^T[GA][CT]AG
SmlI 403 CTCAAG C^T[CT][GA]AG
TfiI 187 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 400 GAGCTC G[GA]GC[CT]^C
467 GGGCTC
BmyI 400 GAGCTC G[AGT]GC[ACT]^C
467 GGGCTC
BsiYI 15 CCCAGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
71 CCCCTCCTGGG
BslI 15 CCCAGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
71 CCCCTCCTGGG
BsmFI 58 GTCCC GGGAC(10/14)
69 GTCCC
Bsp1286I 400 GAGCTC G[AGT]GC[ACT]^C
467 GGGCTC
BstF5I 144 CATCC GGATG(2/0)
170 GGATG
FokI 144 CATCC GGATG(9/13)
170 GGATG
HaeIII 19 GGCC GG^CC
449 GGCC
HapII 168 CCGG C^CGG
465 CCGG
HpaII 168 CCGG C^CGG
465 CCGG
MaeIII 81 GTCAC ^GT[ACGT]AC
193 GTCAC
MspI 168 CCGG C^CGG
465 CCGG
PleI 483 GACTC GAGTC(4/5)
349 GAGTC
Tsp45I 81 GTCAC ^GT[GC]AC
193 GTCAC
TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^
195 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
DdeI EcoRII Fnu4HI HinfI ItaI
MboII MnlI MvaI ScrFI TseI