################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglII BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MflI MluI MroI MseI MslI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 1121 GACGCC G[GA]^CG[CT]C AfaI 1919 GTAC GT^AC Alw44I 1696 GTGCAC G^TGCAC ApaLI 1696 GTGCAC G^TGCAC BanI 1570 GGCACC G^G[CT][GA]CC BbrPI 607 CACGTG CAC^GTG BbsI 1413 GTCTTC GAAGAC(2/6) BglI 1640 GCCACCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 696 GCTGAGC GC^T[ACGT]AGC BpmI 1600 CTGGAG CTGGAG(16/14) Bpu1102I 696 GCTGAGC GC^T[ACGT]AGC BpuAI 1413 GTCTTC GAAGAC(2/6) BsaAI 607 CACGTG [CT]AC^GT[GA] BsaHI 1121 GACGCC G[GA]^CG[CT]C BsiEI 535 CGGCCG CG[GA][CT]^CG BsrFI 368 GCCGGC [GA]^CCGG[CT] BssHII 1675 GCGCGC G^CGCGC BssSI 1184 CTCGTG CACGAG(-5/-1) BstAPI 213 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 696 GCTGAGC GC^T[ACGT]AGC Cfr10I 368 GCCGGC [GA]^CCGG[CT] EagI 535 CGGCCG C^GGCCG EclXI 535 CGGCCG C^GGCCG Eco52I 535 CGGCCG C^GGCCG EcoRV 1030 GATATC GAT^ATC Hin1I 1121 GACGCC G[GA]^CG[CT]C HinfI 328 GACTC G^A[ACGT]TC MaeII 608 ACGT A^CGT NaeI 368 GCCGGC GCC^GGC NgoMIV 368 GCCGGC G^CCGGC NspI 1671 GCATGC [GA]CATG^[CT] PleI 328 GACTC GAGTC(4/5) PmaCI 607 CACGTG CAC^GTG PmlI 607 CACGTG CAC^GTG PvuII 1795 CAGCTG CAG^CTG RsaI 1919 GTAC GT^AC SexAI 1655 ACCTGGT A^CC[AT]GGT SfcI 1891 CTACAG C^T[GA][CT]AG SphI 1671 GCATGC GCATG^C Tsp509I 1476 AATT ^AATT XcmI 153 CCATGGCGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Aor51HI 452 AGCGCT AGC^GCT 1043 AGCGCT AvrII 79 CCTAGG C^CTAGG 1644 CCTAGG BlnI 79 CCTAGG C^CTAGG 1644 CCTAGG BsaI 288 GGTCTC GGTCTC(1/5) 729 GGTCTC BseRI 148 CTCCTC GAGGAG(10/8) 22 GAGGAG BsmBI 1455 CGTCTC CGTCTC(1/5) 737 GAGACG BsrBI 145 CCGCTC CCGCTC(-3/-3) 1834 CCGCTC EarI 1271 CTCTTC CTCTTC(1/4) 430 GAAGAG Eco47III 452 AGCGCT AGC^GCT 1043 AGCGCT Ksp632I 1271 CTCTTC CTCTTC(1/4) 430 GAAGAG KspI 1116 CCGCGG CCGC^GG 1808 CCGCGG PflMI 939 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1483 CCATCCTCTGG SacII 1116 CCGCGG CCGC^GG 1808 CCGCGG TaqI 893 TCGA T^CGA 1682 TCGA TthHB8I 893 TCGA T^CGA 1682 TCGA Van91I 939 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1483 CCATCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AluI AlwNI ApaI AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmaI StyI TseI Tsp45I TspRI XmaCI XmaI