(Circular) (Six-base) MAPSORT of: pFN21AE3664.seq Check: 5534 from: 1 to: 6667 pFN21AE3664 6667 bp With 182 enzymes: SgfI * March 28, 2012 12:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3548 3567 3868 3548 Size: 19 301 6347 Fragments arranged by size: 6347 301 19 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4477 278 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4010 1937 Size: 2073 4594 Acc65I G'GTAC_C Cuts at: 3988 3988 Size: 6667 AclI AA'CG_TT Cuts at: 1563 4794 5167 1563 Size: 3231 373 3063 Fragments arranged by size: 3231 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 2652 3037 3766 4730 6114 6382 1632 Size: 642 378 385 729 964 1384 268 1917 Fragments arranged by size: 1917 1384 964 729 642 385 378 268 AfeI AGC'GCT Cuts at: 2459 3050 2459 Size: 591 6076 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6470 AflIII A'CryG_T Cuts at: 1203 5566 6543 1203 Size: 4363 977 1327 Fragments arranged by size: 4363 1327 977 AgeI A'CCGG_T Cuts at: 5498 5498 Size: 6667 AhdI GACnn_n'nnGTC Cuts at: 5396 5396 Size: 6667 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6667 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3599 1184 Size: 2415 4252 AlwNI CAG_nnn'CTG Cuts at: 2059 2224 2239 2701 4410 5982 2059 Size: 165 15 462 1709 1572 2744 Fragments arranged by size: 2744 1709 1572 462 165 15 ApaI G_GGCC'C Cuts at: 2386 3356 3475 2386 Size: 970 119 5578 Fragments arranged by size: 5578 970 119 ApaLI G'TGCA_C Cuts at: 3701 4724 5880 3701 Size: 1023 1156 4488 Fragments arranged by size: 4488 1156 1023 ApoI r'AATT_y Cuts at: 1493 3976 4202 4354 6463 1493 Size: 2483 226 152 2109 1697 Fragments arranged by size: 2483 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5221 160 Size: 872 4189 1606 Fragments arranged by size: 4189 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 AvaI C'yCGr_G Cuts at: 1870 1943 2184 2265 2367 2504 2682 2737 2836 Size: 73 241 81 102 137 178 55 99 Cuts at: 2836 2977 3522 3952 3992 1870 Size: 141 545 430 40 4545 Fragments arranged by size: 4545 545 430 241 178 141 137 102 99 81 73 55 40 AvrII C'CTAG_G Cuts at: 2084 3649 2084 Size: 1565 5102 BamHI G'GATC_C Cuts at: 3997 4364 3997 Size: 367 6300 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3575 3988 5443 618 Size: 325 217 627 1788 413 1455 1842 Fragments arranged by size: 1842 1788 1455 627 413 325 217 BanII G_rGCy'C Cuts at: 1391 2386 2775 2984 3356 3475 3501 3966 3986 Size: 995 389 209 372 119 26 465 20 Cuts at: 3986 1391 Size: 4072 Fragments arranged by size: 4072 995 465 389 372 209 119 26 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3411 928 Size: 971 1512 4184 Fragments arranged by size: 4184 1512 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4858 4892 1903 Size: 34 2921 34 3678 Fragments arranged by size: 3678 2921 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4559 5769 4559 Size: 1210 5457 BclI T'GATC_A Cuts at: 1548 1548 Size: 6667 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3651 5278 136 Size: 107 122 71 631 605 1979 1627 1525 Fragments arranged by size: 1979 1627 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6662 6662 Size: 6667 BlpI GC'TnA_GC Cuts at: 2702 4084 2702 Size: 1382 5285 Bme1580I G_kGCm'C Cuts at: 1790 2386 3073 3089 3356 3475 3578 3705 4728 Size: 596 687 16 267 119 103 127 1023 Cuts at: 4728 5884 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1023 687 596 267 127 119 103 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6667 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2800 3092 3382 Size: 416 53 83 399 24 1383 292 290 Cuts at: 3382 3604 3945 4398 5356 442 Size: 222 341 453 958 1753 Fragments arranged by size: 1753 1383 958 453 416 399 341 292 290 222 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6667 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2188 2188 Size: 6667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3626 5327 1239 Size: 2387 1701 2579 Fragments arranged by size: 2579 2387 1701 Bpu10I CC'TnA_GC Cuts at: 2106 2106 Size: 6667 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4792 5657 5955 4792 Size: 865 298 5504 Fragments arranged by size: 5504 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2299 2740 5330 882 Size: 617 800 441 2590 2219 Fragments arranged by size: 2590 2219 800 617 441 BsaAI yAC'GTr Cuts at: 493 1204 2614 4458 493 Size: 711 1410 1844 2702 Fragments arranged by size: 2702 1844 1410 711 BsaBI GATnn'nnATC Cuts at: 6661 6661 Size: 6667 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3127 4474 4856 5491 Size: 53 83 186 936 1594 1347 382 635 Cuts at: 5491 275 Size: 1451 Fragments arranged by size: 1594 1451 1347 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5100 5498 5772 5919 6330 5100 Size: 398 274 147 411 5437 Fragments arranged by size: 5437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2975 3925 3955 1178 Size: 30 165 30 1542 30 950 30 3890 Fragments arranged by size: 3890 1542 950 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2144 1186 Size: 856 102 5709 Fragments arranged by size: 5709 856 102 BseYI C'CCAG_C Cuts at: 2070 2179 2454 2460 2636 3094 3223 3288 3365 Size: 109 275 6 176 458 129 65 77 Cuts at: 3365 3383 3571 3641 3711 3802 5870 6366 2070 Size: 18 188 70 70 91 2068 496 2371 Fragments arranged by size: 2371 2068 496 458 275 188 176 129 109 91 77 70 70 65 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 2290 3095 1163 Size: 573 474 80 805 4735 Fragments arranged by size: 4735 805 573 474 80 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2543 4117 4878 5906 1540 Size: 275 186 542 1574 761 1028 2301 Fragments arranged by size: 2301 1574 1028 761 542 275 186 BsiHKAI G_wGCw'C Cuts at: 2093 3624 3705 3861 4728 4813 5884 2093 Size: 1531 81 156 867 85 1071 2876 Fragments arranged by size: 2876 1531 1071 867 156 85 81 BsmI GAATG_Cn' Cuts at: 4178 4271 4178 Size: 93 6574 BsmBI CGTCTCn'nnnn_ Cuts at: 2736 3466 2736 Size: 730 5937 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2386 2775 2984 3073 3089 3356 Size: 399 303 293 389 209 89 16 267 Cuts at: 3356 3475 3501 3578 3624 3705 3861 3966 3986 Size: 119 26 77 46 81 156 105 20 Cuts at: 3986 4728 4813 5884 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 389 303 293 267 209 156 119 105 89 85 81 77 46 26 20 16 BspEI T'CCGG_A Cuts at: 6330 6330 Size: 6667 BspHI T'CATG_A Cuts at: 4556 6286 4556 Size: 1730 4937 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2538 2964 3166 3279 3548 3567 3868 4022 Size: 1694 426 202 113 269 19 301 154 Cuts at: 4022 844 Size: 3489 Fragments arranged by size: 3489 1694 426 301 269 202 154 113 19 BsrBI CCG'CTC Cuts at: 1394 1823 2152 3841 4554 1394 Size: 429 329 1689 713 3507 Fragments arranged by size: 3507 1689 713 429 329 BsrDI GCAATG_nn' Cuts at: 65 1248 5162 5336 65 Size: 1183 3914 174 1396 Fragments arranged by size: 3914 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2373 5311 5498 1700 Size: 110 563 2938 187 2869 Fragments arranged by size: 2938 2869 563 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6667 BssHII G'CGCG_C Cuts at: 1925 3680 1925 Size: 1755 4912 BssSI C'ACGA_G Cuts at: 3189 4727 5739 3189 Size: 1538 1012 4117 Fragments arranged by size: 4117 1538 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2224 794 Size: 1430 5237 BstBI TT'CG_AA Cuts at: 5471 5471 Size: 6667 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6667 BstYI r'GATC_y Cuts at: 1972 3997 4364 4749 4766 6218 6304 6316 6662 Size: 2025 367 385 17 1452 86 12 346 Cuts at: 6662 1972 Size: 1977 Fragments arranged by size: 2025 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2077 2112 2158 3121 3234 3762 3813 Size: 552 1012 35 46 963 113 528 51 Cuts at: 3813 513 Size: 3367 Fragments arranged by size: 3367 1012 963 552 528 113 51 46 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2199 487 Size: 683 56 973 4955 Fragments arranged by size: 4955 973 683 56 BtsI GCAGTG_nn' Cuts at: 2466 2498 2603 2865 3073 3107 3410 3926 4183 Size: 32 105 262 208 34 303 516 257 Cuts at: 4183 4977 4997 2466 Size: 794 20 4136 Fragments arranged by size: 4136 794 516 303 262 257 208 105 34 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6667 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6632 DraI TTT'AAA Cuts at: 3971 3971 Size: 6667 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5674 809 Size: 507 4358 1802 Fragments arranged by size: 4358 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2540 2850 Size: 54 1425 50 275 18 456 254 310 Cuts at: 2850 2941 3630 3759 4114 5003 6632 8 Size: 91 689 129 355 889 1629 43 Fragments arranged by size: 1629 1425 889 689 456 355 310 275 254 129 91 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2540 4114 1537 Size: 275 728 1574 4090 Fragments arranged by size: 4090 1574 728 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2430 3282 4597 1353 Size: 1077 852 1315 3423 Fragments arranged by size: 3423 1315 1077 852 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3818 5254 5628 5774 387 Size: 1064 138 2229 1436 374 146 1280 Fragments arranged by size: 2229 1436 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2274 2652 3037 3626 3766 4730 5327 Size: 393 642 378 385 589 140 964 597 Cuts at: 5327 6114 6382 1239 Size: 787 268 1524 Fragments arranged by size: 1524 964 787 642 597 589 393 385 378 268 140 EcoO109I rG'GnC_Cy Cuts at: 2221 2262 2382 2584 3057 3352 3471 3472 3531 Size: 41 120 202 473 295 119 1 59 Cuts at: 3531 2221 Size: 5357 Fragments arranged by size: 5357 473 295 202 120 119 59 41 1 EcoRI G'AATT_C Cuts at: 3976 4354 3976 Size: 378 6289 EcoRV GAT'ATC Cuts at: 3037 3037 Size: 6667 FspI TGC'GCA Cuts at: 5173 5173 Size: 6667 HaeII r_GCGC'y Cuts at: 1678 2461 3052 3657 5814 1678 Size: 783 591 605 2157 2531 Fragments arranged by size: 2531 2157 783 605 591 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2945 2977 5308 5340 5382 Size: 32 135 32 1373 32 2331 32 42 Cuts at: 5382 5414 1373 Size: 32 2626 Fragments arranged by size: 2626 2331 1373 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4011 4257 669 Size: 988 281 2073 246 3079 Fragments arranged by size: 3079 2073 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6667 HpaI GTT'AAC Cuts at: 4257 4257 Size: 6667 KpnI G_GTAC'C Cuts at: 3992 3992 Size: 6667 MfeI C'AATT_G Cuts at: 4266 4266 Size: 6667 MluI A'CGCG_T Cuts at: 6543 6543 Size: 6667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3180 5781 5965 1305 Size: 1875 2601 184 2007 Fragments arranged by size: 2601 2007 1875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2943 3632 3761 10 Size: 54 1425 343 1111 689 129 2916 Fragments arranged by size: 2916 1425 1111 689 343 129 54 MslI CAynn'nnrTG Cuts at: 518 1166 4625 4984 5143 518 Size: 648 3459 359 159 2042 Fragments arranged by size: 3459 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2236 2339 2988 3123 3397 3571 3583 3802 Size: 630 103 649 135 274 174 12 219 Cuts at: 3802 3815 4083 4760 5908 6153 1606 Size: 13 268 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 649 630 274 268 245 219 174 135 103 13 12 NaeI GCC'GGC Cuts at: 2375 2375 Size: 6667 NcoI C'CATG_G Cuts at: 513 1065 2112 2158 3234 3762 513 Size: 552 1047 46 1076 528 3418 Fragments arranged by size: 3418 1076 1047 552 528 46 NdeI CA'TA_TG Cuts at: 387 387 Size: 6667 NgoMIV G'CCGG_C Cuts at: 2373 2373 Size: 6667 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6667 NotI GC'GGCC_GC Cuts at: 4114 4114 Size: 6667 NspI r_CATG'y Cuts at: 1132 1229 3680 4025 5570 1132 Size: 97 2451 345 1545 2229 Fragments arranged by size: 2451 2229 1545 345 97 PasI CC'CwG_GG Cuts at: 1348 2890 1348 Size: 1542 5125 PciI A'CATG_T Cuts at: 5566 5566 Size: 6667 PflMI CCAn_nnn'nTGG Cuts at: 1108 2950 3494 1108 Size: 1842 544 4281 Fragments arranged by size: 4281 1842 544 PmeI GTTT'AAAC Cuts at: 3971 3971 Size: 6667 PmlI CAC'GTG Cuts at: 2614 2614 Size: 6667 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4736 4768 6249 6281 4736 Size: 32 1481 32 5122 Fragments arranged by size: 5122 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6667 PsiI TTA'TAA Cuts at: 4237 4237 Size: 6667 PspOMI G'GGCC_C Cuts at: 2382 3352 3471 2382 Size: 970 119 5578 Fragments arranged by size: 5578 970 119 PstI C_TGCA'G Cuts at: 830 4019 830 Size: 3189 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6667 PvuII CAG'CTG Cuts at: 3802 3802 Size: 6667 SacII CC_GC'GG Cuts at: 3124 3816 3124 Size: 692 5975 SalI G'TCGA_C Cuts at: 1936 4009 1936 Size: 2073 4594 SbfI CC_TGCA'GG Cuts at: 4019 4019 Size: 6667 ScaI AGT'ACT Cuts at: 4915 6627 4915 Size: 1712 4955 SexAI A'CCwGG_T Cuts at: 3660 3660 Size: 6667 SfcI C'TryA_G Cuts at: 826 1045 3896 4015 5150 5831 6022 826 Size: 219 2851 119 1135 681 191 1471 Fragments arranged by size: 2851 1471 1135 681 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6667 SmaI CCC'GGG Cuts at: 1872 2267 2506 2684 3524 3954 3994 1872 Size: 395 239 178 840 430 40 4545 Fragments arranged by size: 4545 840 430 395 239 178 40 SmlI C'TyrA_G Cuts at: 820 1017 1943 4771 5672 5934 820 Size: 197 926 2828 901 262 1553 Fragments arranged by size: 2828 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4458 493 Size: 3965 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6667 SphI G_CATG'C Cuts at: 1132 3680 4025 1132 Size: 2548 345 3774 Fragments arranged by size: 3774 2548 345 SspI AAT'ATT Cuts at: 5 52 4591 5 Size: 47 4539 2081 Fragments arranged by size: 4539 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6667 StyI C'CwwG_G Cuts at: 513 1065 2084 2112 2158 3060 3234 3438 3649 Size: 552 1019 28 46 902 174 204 211 Cuts at: 3649 3762 4106 513 Size: 113 344 3074 Fragments arranged by size: 3074 1019 902 552 344 211 204 174 113 46 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2961 3586 3703 4694 4711 4864 5049 Size: 1255 1381 625 117 991 17 153 185 Cuts at: 5049 5494 325 Size: 445 1498 Fragments arranged by size: 1498 1381 1255 991 625 445 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4913 6625 96 Size: 274 80 33 51 1443 2936 1712 138 Fragments arranged by size: 2936 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3925 3957 6627 6659 3925 Size: 32 2670 32 3933 Fragments arranged by size: 3933 2670 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6667 XbaI T'CTAG_A Cuts at: 4003 4003 Size: 6667 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 6667 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6667 XmaI C'CCGG_G Cuts at: 1870 2265 2504 2682 3522 3952 3992 1870 Size: 395 239 178 840 430 40 4545 Fragments arranged by size: 4545 840 430 395 239 178 40 XmnI GAAnn'nnTTC Cuts at: 4796 4796 Size: 6667 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4475 276 Size: 53 83 186 936 2941 2468 Fragments arranged by size: 2941 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI