################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstNI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MspI MunI MvaI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 43 CGCG CG^CG AcsI 215 AAATTT [GA]^AATT[CT] ApoI 215 AAATTT [GA]^AATT[CT] AvaII 70 GGACC G^G[AT]CC BbsI 145 GTCTTC GAAGAC(2/6) BcgI 45 CGATCCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 145 GTCTTC GAAGAC(2/6) BsaBI 334 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 254 GTCTC GTCTC(1/5) Bsp1407I 159 TGTACA T^GTACA BspMI 51 ACCTGC ACCTGC(4/8) BsrDI 436 GCAATG GCAATG(2/0) BsrGI 159 TGTACA T^GTACA BsrI 95 ACTGG ACTGG(1/-1) BstEII 278 GGTCACC G^GT[ACGT]ACC BstF5I 223 CATCC GGATG(2/0) BstPI 278 GGTCACC G^GT[ACGT]ACC BstUI 43 CGCG CG^CG BstXI 104 CCACTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 99 GCAAGC GC[ACGT]^[ACGT]GC DraI 184 TTTAAA TTT^AAA DraII 114 GGGGCCT [GA]G^G[ACGT]CC[CT] EarI 9 GAAGAG CTCTTC(1/4) EcoO109I 114 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 278 GGTCACC G^GT[ACGT]ACC FokI 223 CATCC GGATG(9/13) HaeII 3 GGCGCT [GA]GCGC^[CT] HaeIII 116 GGCC GG^CC HphI 280 TCACC GGTGA(8/7) Ksp632I 9 GAAGAG CTCTTC(1/4) MamI 334 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 185 TTAA T^TAA MslI 105 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 65 CAGCTG C[AC]G^C[GT]G MvnI 43 CGCG CG^CG NdeI 315 CATATG CA^TATG NlaIII 409 CATG CATG^ NlaIV 114 GGGGCC GG[ACGT]^[ACGT]CC NspI 408 ACATGC [GA]CATG^[CT] PvuII 65 CAGCTG CAG^CTG SfcI 37 CTACAG C^T[GA][CT]AG SspBI 159 TGTACA T^GTACA TaqI 257 TCGA T^CGA TfiI 15 GATTC G^A[AT]TC Tru9I 185 TTAA T^TAA TthHB8I 257 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 160 GTAC GT^AC 358 GTAC AluI 66 AGCT AG^CT 285 AGCT AlwI 46 GATCC GGATC(4/5) 334 GATCC Cfr13I 70 GGACC G^G[ACGT]CC 115 GGGCC MaeIII 270 GTCAC ^GT[ACGT]AC 279 GTCAC MboII 9 GAAGA GAAGA(8/7) 146 TCTTC PleI 423 GACTC GAGTC(4/5) 143 GAGTC RsaI 160 GTAC GT^AC 358 GTAC Sau96I 70 GGACC G^G[ACGT]CC 115 GGGCC Tsp45I 270 GTCAC ^GT[GC]AC 279 GTCAC Tsp509I 26 AATT ^AATT 216 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### CfoI DdeI DpnII HhaI HinP1I HinfI MboI MnlI NdeII Sau3AI TspRI