################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmeI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TseI Tsp509I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Alw44I 364 GTGCAC G^TGCAC ApaLI 364 GTGCAC G^TGCAC AspI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 282 GGACC G^G[AT]CC BbrPI 217 CACGTG CAC^GTG BcnI 285 CCCGG CC^[GC]GG BpmI 88 CTGGAG CTGGAG(16/14) BsaAI 217 CACGTG [CT]AC^GT[GA] BsgI 259 CTGCAC GTGCAG(16/14) BsmAI 9 GTCTC GTCTC(1/5) BspMI 257 ACCTGC ACCTGC(4/8) BssHII 56 GCGCGC G^CGCGC BstEII 321 GGTGACC G^GT[ACGT]ACC BstF5I 95 GGATG GGATG(2/0) BstPI 321 GGTGACC G^GT[ACGT]ACC BstXI 203 CCAACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 83 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 83 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 321 GGTGACC G^GT[ACGT]ACC FokI 95 GGATG GGATG(9/13) HapII 286 CCGG C^CGG HgaI 193 GACGC GACGC(5/10) HincII 289 GTCAAC GT[CT]^[GA]AC HindII 289 GTCAAC GT[CT]^[GA]AC HindIII 271 AAGCTT A^AGCTT HpaII 286 CCGG C^CGG MaeIII 322 GTGAC ^GT[ACGT]AC MseI 167 TTAA T^TAA MspI 286 CCGG C^CGG NciI 285 CCCGG CC^[GC]GG NlaIII 228 CATG CATG^ NlaIV 282 GGACCC GG[ACGT]^[ACGT]CC NspI 227 ACATGC [GA]CATG^[CT] PflFI 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 217 CACGTG CAC^GTG PmlI 217 CACGTG CAC^GTG PshAI 24 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC TaqI 140 TCGA T^CGA Tru9I 167 TTAA T^TAA Tsp45I 322 GTGAC ^GT[GC]AC TspRI 310 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 283 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 140 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 272 AGCT AG^CT 299 AGCT AspHI 364 GTGCAC G[AT]GC[AT]^C 399 GAGCAC BcgI 69 CGAGTATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 267 GCACAAGCTTCG BmyI 364 GTGCAC G[AGT]GC[ACT]^C 399 GAGCAC BsiHKAI 364 GTGCAC G[AT]GC[AT]^C 399 GAGCAC BsiYI 86 CCCTGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 162 CCAGGTTAAGG BslI 86 CCCTGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 162 CCAGGTTAAGG Bsp1286I 364 GTGCAC G[AGT]GC[ACT]^C 399 GAGCAC EaeI 188 TGGCCG [CT]^GGCC[GA] 329 TGGCCG FauI 132 CCCGC CCCGC(4/6) 343 CCCGC Fnu4HI 37 GCCGC GC^[ACGT]GC 331 GCCGC HphI 321 GGTGA GGTGA(8/7) 353 TCACC ItaI 37 GCCGC GC^[ACGT]GC 331 GCCGC MaeII 29 ACGT A^CGT 218 ACGT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccII AciI BsaJI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EcoRII HaeIII HhaI HinP1I MnlI MvaI MvnI MwoI Sau96I ScrFI