################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 72 GAATTT [GA]^AATT[CT] AfaI 54 GTAC GT^AC AhdI 117 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 11 AGCT AG^CT ApoI 72 GAATTT [GA]^AATT[CT] AspEI 117 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvI 241 GCAGC GCAGC(8/12) BfaI 112 CTAG C^TAG BpmI 13 CTGGAG CTGGAG(16/14) BsaI 115 GAGACC GGTCTC(1/5) BsmAI 115 GAGAC GTCTC(1/5) BssKI 204 CCAGG ^CC[ACGT]GG BstF5I 93 GGATG GGATG(2/0) BstNI 204 CCAGG CC^[AT]GG DdeI 128 CTCAG C^T[ACGT]AG Eam1105I 117 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 60 CCTGATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 204 CCAGG ^CC[AT]GG EcoRV 175 GATATC GAT^ATC Fnu4HI 241 GCAGC GC^[ACGT]GC FokI 93 GGATG GGATG(9/13) HpaI 196 GTTAAC GTT^AAC HphI 222 GGTGA GGTGA(8/7) ItaI 241 GCAGC GC^[ACGT]GC MaeI 112 CTAG C^TAG MseI 197 TTAA T^TAA MslI 216 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 204 CCAGG CC^[AT]GG RsaI 54 GTAC GT^AC ScaI 53 AGTACT AGT^ACT ScrFI 204 CCAGG CC^[ACGT]GG SfcI 87 CTACAG C^T[GA][CT]AG TaqI 38 TCGA T^CGA Tru9I 197 TTAA T^TAA TseI 241 GCAGC G^C[AT]GC TspRI 183 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 38 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AatII 40 GACGTC GACGT^C 154 GACGTC AccI 43 GTCTAC GT^[AC][GT]AC 85 GTCTAC AcyI 40 GACGTC G[GA]^CG[CT]C 154 GACGTC BsaHI 40 GACGTC G[GA]^CG[CT]C 154 GACGTC BsgI 149 GTGCAG GTGCAG(16/14) 191 GTGCAG Hin1I 40 GACGTC G[GA]^CG[CT]C 154 GACGTC HincII 3 GTTGAC GT[CT]^[GA]AC 196 GTTAAC HindII 3 GTTGAC GT[CT]^[GA]AC 196 GTTAAC MaeII 41 ACGT A^CGT 155 ACGT MboII 102 GAAGA GAAGA(8/7) 262 GAAGA MnlI 127 CCTC CCTC(7/6) 213 CCTC NlaIII 79 CATG CATG^ 259 CATG Tsp509I 73 AATT ^AATT 107 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### BsiYI BslI