Restriction map for
pFN21AE1525
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 593 CGCG CG^CG
AciI 592 CCGC CCGC(-3/-1)
AflIII 148 ACATGT A^C[GA][CT]GT
AhdI 550 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 327 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 550 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 392 GGTGCC G^G[CT][GA]CC
BanII 562 GAGCCC G[GA]GC[CT]^C
BbvCI 355 GCTGAGG CCTCAGC(-5/-2)
BmyI 562 GAGCCC G[AGT]GC[ACT]^C
BpmI 56 CTCCAG CTGGAG(16/14)
BsaI 93 GGTCTC GGTCTC(1/5)
BsmFI 76 GTCCC GGGAC(10/14)
BsmI 243 GAATGC GAATGC(1/-1)
Bsp1286I 562 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 419 TGTACA T^GTACA
BspLU11I 148 ACATGT A^CATGT
BspMI 684 GCAGGT ACCTGC(4/8)
BsrGI 419 TGTACA T^GTACA
BstUI 593 CGCG CG^CG
Cac8I 374 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 372 GGGCC G^G[ACGT]CC
DpnII 429 GATC ^GATC
DraI 124 TTTAAA TTT^AAA
Eam1105I 550 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MboI 429 GATC ^GATC
MspA1I 544 CAGCTG C[AC]G^C[GT]G
MvnI 593 CGCG CG^CG
NdeII 429 GATC ^GATC
NspI 148 ACATGT [GA]CATG^[CT]
PflMI 182 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 544 CAGCTG CAG^CTG
Sau3AI 429 GATC ^GATC
Sau96I 372 GGGCC G^G[ACGT]CC
SmlI 454 CTCAAG C^T[CT][GA]AG
SspBI 419 TGTACA T^GTACA
StuI 347 AGGCCT AGG^CCT
Van91I 182 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 222 AAATTC [GA]^AATT[CT]
250 AAATTT
AocI 88 CCTAAGG CC^T[ACGT]AGG
387 CCTCAGG
ApoI 222 AAATTC [GA]^AATT[CT]
250 AAATTT
Asp700I 159 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
720 GAAAGGCTTC
BsiYI 182 CCACCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
624 CCCATTGTTGG
BslI 182 CCACCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
624 CCCATTGTTGG
BsmAI 217 GAGAC GTCTC(1/5)
94 GTCTC
BssKI 315 CCTGG ^CC[ACGT]GG
716 CCAGG
BstNI 315 CCTGG CC^[AT]GG
716 CCAGG
Bsu36I 88 CCTAAGG CC^T[ACGT]AGG
387 CCTCAGG
EarI 382 CTCTTC CTCTTC(1/4)
214 GAAGAG
Eco81I 88 CCTAAGG CC^T[ACGT]AGG
387 CCTCAGG
EcoRII 315 CCTGG ^CC[AT]GG
716 CCAGG
HaeIII 348 GGCC GG^CC
373 GGCC
Ksp632I 382 CTCTTC CTCTTC(1/4)
214 GAAGAG
MvaI 315 CCTGG CC^[AT]GG
716 CCAGG
PleI 141 GACTC GAGTC(4/5)
556 GAGTC
ScaI 458 AGTACT AGT^ACT
602 AGTACT
ScrFI 315 CCTGG CC^[ACGT]GG
716 CCAGG
SspI 120 AATATT AAT^ATT
235 AATATT
TfiI 16 GAATC G^A[AT]TC
639 GATTC
XmnI 159 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
720 GAAAGGCTTC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BbsI BbvI BpuAI
BsaJI BsrI BstF5I DdeI DsaI
EcoT14I Fnu4HI FokI HinfI HphI
ItaI MaeIII MboII MnlI MseI
NcoI NlaIII NlaIV RsaI StyI
Tru9I TseI Tsp45I Tsp509I TspRI