(Circular) (Six-base) MAPSORT of: pFN21AE1525.seq Check: 2466 from: 1 to: 5476 pFN21AE1525 5476 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 2775 3603 3976 1563 Size: 1212 828 373 3063 Fragments arranged by size: 3063 1212 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2314 2333 2716 3539 4923 5191 1632 Size: 682 19 383 823 1384 268 1917 Fragments arranged by size: 1917 1384 823 682 383 268 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 2153 4375 5352 1203 Size: 950 2222 977 1327 Fragments arranged by size: 2222 1327 977 950 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 2560 4205 2560 Size: 1645 3831 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 2337 3219 4791 2337 Size: 882 1572 3022 Fragments arranged by size: 3022 1572 882 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2227 2255 2785 3011 3163 5272 1493 Size: 734 28 530 226 152 2109 1697 Fragments arranged by size: 2109 1697 734 530 226 152 28 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2801 1870 Size: 73 858 4545 Fragments arranged by size: 4545 858 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2300 2333 2300 Size: 33 5443 BamHI G'GATC_C Cuts at: 2806 3173 2806 Size: 367 5109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2397 2797 4252 618 Size: 325 217 627 610 400 1455 1842 Fragments arranged by size: 1842 1455 627 610 400 325 217 BanII G_rGCy'C Cuts at: 1391 2571 2795 1391 Size: 1180 224 4072 Fragments arranged by size: 4072 1180 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 2320 2517 928 Size: 971 369 52 197 3887 Fragments arranged by size: 3887 971 369 197 52 BbvCI CC'TCA_GC Cuts at: 2361 2361 Size: 5476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 3537 4693 1790 Size: 1747 1156 2573 Fragments arranged by size: 2573 1747 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2425 3207 4165 Size: 416 53 83 399 24 1008 782 958 Cuts at: 4165 442 Size: 1753 Fragments arranged by size: 1753 1008 958 782 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 4136 1239 Size: 807 2090 2579 Fragments arranged by size: 2579 2090 807 Bpu10I CC'TnA_GC Cuts at: 2361 2754 2361 Size: 393 5083 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2444 3601 4466 4764 2444 Size: 1157 865 298 3156 Fragments arranged by size: 3156 1157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2104 4139 882 Size: 617 605 2035 2219 Fragments arranged by size: 2219 2035 617 605 BsaAI yAC'GTr Cuts at: 493 1204 3267 493 Size: 711 2063 2702 Fragments arranged by size: 2702 2063 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3283 3665 4300 275 Size: 53 83 186 936 1750 382 635 1451 Fragments arranged by size: 1750 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 2471 2501 1178 Size: 30 165 30 724 30 314 30 4153 Fragments arranged by size: 4153 724 314 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5476 BseYI C'CCAG_C Cuts at: 4679 5175 4679 Size: 496 4980 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4903 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2926 3687 4715 1540 Size: 275 186 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3537 3622 4693 3537 Size: 85 1071 4320 Fragments arranged by size: 4320 1071 85 BsmI GAATG_Cn' Cuts at: 2254 2987 3080 2254 Size: 733 93 4650 Fragments arranged by size: 4650 733 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2571 2795 3537 3622 4693 1391 Size: 399 781 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 781 742 399 224 85 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2680 2831 844 Size: 1836 151 3489 Fragments arranged by size: 3489 1836 151 BsrBI CCG'CTC Cuts at: 1394 1823 3363 1394 Size: 429 1540 3507 Fragments arranged by size: 3507 1540 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3971 4145 65 Size: 1183 2723 174 1396 Fragments arranged by size: 2723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4120 4307 1700 Size: 110 2310 187 2869 Fragments arranged by size: 2869 2310 187 110 BsrGI T'GTAC_A Cuts at: 96 2424 96 Size: 2328 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471 Size: 834 367 385 17 1452 86 12 346 Cuts at: 5471 1972 Size: 1977 Fragments arranged by size: 1977 1452 834 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2094 2393 2094 Size: 299 5177 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2114 2401 513 Size: 552 939 12 98 287 3588 Fragments arranged by size: 3588 939 552 287 98 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3786 3806 2992 Size: 794 20 4662 Fragments arranged by size: 4662 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2131 2780 2131 Size: 649 4827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2923 3812 5441 Size: 54 1425 50 275 18 1093 889 1629 Cuts at: 5441 8 Size: 43 Fragments arranged by size: 1629 1425 1093 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2923 1537 Size: 275 1111 4090 Fragments arranged by size: 4090 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 2393 3406 1353 Size: 861 179 1013 3423 Fragments arranged by size: 3423 1013 861 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2314 2333 2716 3539 4136 4923 Size: 393 414 268 19 383 823 597 787 Cuts at: 4923 5191 1239 Size: 268 1524 Fragments arranged by size: 1524 823 787 597 414 393 383 268 268 19 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2119 2119 Size: 5476 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 4623 1678 Size: 2945 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 2658 2690 4117 Size: 32 135 32 974 32 80 32 1427 Cuts at: 4117 4149 4191 4223 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1427 974 135 80 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2387 4590 4774 1305 Size: 1082 2203 184 2007 Fragments arranged by size: 2203 2007 1082 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3654 Fragments arranged by size: 3654 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3434 3793 3952 518 Size: 648 2268 359 159 2042 Fragments arranged by size: 2268 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2551 2892 3569 4717 4962 1606 Size: 945 341 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 677 341 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2016 2114 2401 513 Size: 552 939 12 98 287 3588 Fragments arranged by size: 3588 939 552 287 98 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2157 2834 4379 1132 Size: 97 928 677 1545 2229 Fragments arranged by size: 2229 1545 928 677 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5476 PciI A'CATG_T Cuts at: 2153 4375 2153 Size: 2222 3254 PflMI CCAn_nnn'nTGG Cuts at: 1108 2193 1108 Size: 1085 4391 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 2442 3046 2442 Size: 604 4872 PstI C_TGCA'G Cuts at: 830 2828 830 Size: 1998 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 PvuII CAG'CTG Cuts at: 2551 2551 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 2465 2609 3724 5436 2465 Size: 144 1115 1712 2505 Fragments arranged by size: 2505 1712 1115 144 SfcI C'TryA_G Cuts at: 826 1045 2824 3959 4640 4831 826 Size: 219 1779 1135 681 191 1471 Fragments arranged by size: 1779 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2459 3580 4481 4743 820 Size: 197 926 516 1121 901 262 1553 Fragments arranged by size: 1553 1121 926 901 516 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 2127 2242 3400 5 Size: 47 2075 115 1158 2081 Fragments arranged by size: 2081 2075 1158 115 47 StuI AGG'CCT Cuts at: 1517 2354 1517 Size: 837 4639 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2114 2401 2915 513 Size: 552 939 12 98 287 514 3074 Fragments arranged by size: 3074 939 552 514 287 98 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3520 3673 3858 4303 325 Size: 1255 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2463 2607 Size: 274 80 33 51 1443 447 39 144 Cuts at: 2607 3722 5434 96 Size: 1115 1712 138 Fragments arranged by size: 1712 1443 1115 447 274 144 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2471 2503 5436 5468 2127 Size: 32 312 32 2933 32 2135 Fragments arranged by size: 2933 2135 312 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 2168 2729 3605 2168 Size: 561 876 4039 Fragments arranged by size: 4039 876 561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI